Human Gas6 Biotinylated Antibody Summary
Asp118-Ala678
Accession # NP_000811
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Gas6
Gas6 (Growth Arrest Specific 6) is a multimodular protein that is upregulated by a wide variety of cell types in response to growth arrest (1). Gas6 and the structurally related Protein S are vitamin K-dependent and have an extensively gamma -carboxylated N-terminal Gla domain, four EGF-like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG) (2). Human Gas6 is a 75 kDa protein that shares 77-79% amino acid (aa) sequence identity with mouse and rat Gas6 and 43% aa sequence identity with human protein S (over the region expressed). Alternate splicing generates isoforms that lack the Gla domain and/or the spacer between the EGF-like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer (3-5). Human Gas6 interacts with both mouse and rat orthologs of these receptors (1). The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl (6). Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors (7, 8). Gas6 induces a variety of responses, including prevention of apoptosis (9), cell proliferation (10), platelet-mediated thrombosis (11), retinal epithelial cell phagocytosis of outer rod segments (12), inhibition of VEGF‑induced endothelial cell chemotaxis (13), and the differentiation and expansion of NK cell precursors (14). The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells (15). Several of these effects have been shown to require gamma -carboxylation of the Gla domain (12, 16).
- Hafizi, S. and B. Dahlback (2006) FEBS J. 273:5231.
- Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
- Stitt, T.N. et al. (1995) Cell 80:661.
- Ohashi, K. et al. (1995) J. Biol. Chem. 270:22681.
- Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
- Goruppi, S. et al. (1997) FEBS Lett. 415:59.
- Sather, S. et al. (2007) Blood 109:1026.
- Budagian, V. et al. (2005) Mol. Cell. Biol. 25:9324.
- Shankar, S.L. et al. (2006) J. Neurosci. 26:5638.
- Yanagita, M. et al. (2002) J. Clin. Invest. 110:239.
- Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733.
- Hall, M.O. et al. (2002) Exp. Eye Res. 75:391.
- Gallicchio, M. et al. (2005) Blood 105:1970.
- Caraux, A. et al. (2006) Nat. Immunol. 7:747.
- Wu, Y. et al. (2005) J. Cell Sci. 118:539.
- Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790.
Product Datasheets
Citations for Human Gas6 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Role of Gas6 and TAM Receptors in the Identification of Cardiopulmonary Involvement in Systemic Sclerosis and Scleroderma Spectrum Disorders
Authors: M Bellan, A Dimagli, C Piccinino, A Giubertoni, A Ianniello, F Grimoldi, M Sguazzotti, A Nerviani, M Barini, A Carriero, C Smirne, ME Burlone, C Rigamonti, R Minisini, L Salmi, MN Barbaglia, LM Castello, D Sola, P Marino, GC Avanzi, M Pirisi, PP Sainaghi
Dis. Markers, 2020-05-12;2020(0):2696173.
Species: Human
Sample Types: Plasma
Applications: ELISA (detection) -
IL-17 stimulates differentiation of human anti-inflammatory macrophages and phagocytosis of apoptotic neutrophils in response to IL-10 and glucocorticoids.
Authors: Zizzo G, Cohen P
J Immunol, 2013-04-17;190(10):5237-46.
Species: Human
Sample Types: Cell Culture Supernates
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Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.
Authors: Zizzo G, Hilliard B, Monestier M, Cohen P
J Immunol, 2012-08-31;189(7):3508-20.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Plasma protein growth arrest-specific 6 levels are associated with altered glucose tolerance, inflammation, and endothelial dysfunction.
Authors: Hung YJ, Lee CH, Chu NF
Diabetes Care, 2010-05-26;33(8):1840-4.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Gas6 evaluation in patients with acute dyspnea due to suspected pulmonary embolism.
Authors: Sainaghi PP, Alciato F, Carnieletto S, Castello L, Bergamasco L, Sola D, Bongo AS, Inglese E, Polosa R, Avanzi GC
Respir Med, 2008-11-25;103(4):589-94.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Development of a novel immunoassay for the assessment of plasma Gas6 concentrations and their variation with hormonal status.
Authors: Clauser S, Peyrard S, Gaussem P, Crespin M, Emmerich J, Aiach M, Borgel D
Clin. Chem., 2007-08-23;53(10):1808-13.
Species: Human
Sample Types: Plasma
Applications: ELISA Development
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