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Human GATA-1 Antibody

Catalog #: MAB1779
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1779
MAB1779-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GATA-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GATA-1 in direct ELISAs. In Western blots, this antibody does not cross-react with rhGATA-2 or rhGATA-6.
Source
Monoclonal Rat IgG2B Clone # 234732
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human GATA-1
Met1-Ser413
Accession # P15976
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human GATA‑1
Immunocytochemistry
8-25 µg/mL
Immersion-fixed MG-63 human osteosarcoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATA-1

GATA-1 is the founding member of the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue‑specific programs of gene expression and morphogenesis. GATA-1 is expressed in blood forming cells. It interacts with several additional proteins to activate or repress gene expression and is essential for erythropoiesis (1).

References
  1. Patient, R.K. and J. McGhee (2002) Curr. Opin. Genet. Dev. 12:416.
Entrez Gene IDs
2623 (Human); 14460 (Mouse)
Alternate Names
Eryf1; ERYF1GATA-binding protein 1 (globin transcription factor 1); GATA binding protein 1 (globin transcription factor 1); GATA1; GATA-1; GATA-1erythroid transcription factor; GATA-binding factor 1; GF-1; GF1globin transcription factor 1; NF-E1 DNA-binding protein; NFE1erythroid transcription factor 1; transcription factor GATA1; XLTT

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Citation for Human GATA-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. GATA-1 is essential in EGF-mediated induction of nucleotide excision repair activity and ERCC1 expression through ERK2 in human hepatoma cells.
    Authors: Andrieux</LastName><ForeN LO</Initia, Andrieux LO, Fautrel A, Bessard A, Guillouzo A, Baffet G, Langouet S
    Cancer Res., 2007-03-01;67(5):2114-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunoprecipitation

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