Human GATA-3 Alexa Fluor® 594-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: GATA-3

GATA-3 is a 54-60 kDa member of the GATA family of transcription regulating factors. There are currently six mammalian members, each of which binds to a G-A-T-A motif found in gene promoters. Although GATA-3 is traditionally described as being a hematopoietic transcriptional regulator, it has been found in multiple cell types, both embryonic and adult. Postnatal cells reported to express GATA-3 include NK cells, ILCs, NKT cells, B cells, thymocytes (DN, CD4 SP, and CD8 SP) and T cells, plus keratinocytes, sympathetic neurons, renal distal convoluted tubule and mammary duct epithelium. Human GATA-3 is 443 amino acids (aa) in length. It contains two GAGA-type Zn finger domains (aa 263-287 and 317-341) and multiple phosphorylation sites. GATAs as a group are known to either activate, or repress, gene expression, maintain transcriptional activity, and regulate gene expression levels. Within this framework, GATA-3 is best known to drive naïve CD4+ T cells into a Th2 phenotype, induce Th2 proliferation, and inhibit Th1 cell development via T-bet repression. Other effects attributed to GATA-33 include the promotion of Th9 and Treg formation, and the inhibition of Th1, TH17 and B cell development. Over aa 135-258, human and mouse GATA-3 share 94% aa sequence identity.

Product Datasheets

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Related Research Areas

• gamma delta T Cells
• GATA Transcription Factors
• Group 2 ILCs
• Hematopoietic Stem Cell Transcription Factors and Regulators
• Multiple Sclerosis and Myelin Related Molecules
• Natural Killer T (NKT) Cells
• Pluripotent Stem Cell Transcription Factors
• Signaling Molecules and Transcriptional Regulators of Tfh Cell Differentiation
• Th2 Cells
• Transcription Factors in the Akt Pathway
• Transcriptional Regulators in Allergy and Asthma