Human GATA-3 APC-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: GATA-3

GATA-3 is a 54-60 kDa member of the GATA family of transcription regulating factors. There are currently six mammalian members, each of which binds to a G-A-T-A motif found in gene promoters. Although GATA-3 is traditionally described as being a hematopoietic transcriptional regulator, it has been found in multiple cell types, both embryonic and adult. Postnatal cells reported to express GATA-3 include NK cells, ILCs, NKT cells, B cells, thymocytes (DN, CD4 SP, and CD8 SP) and T cells, plus keratinocytes, sympathetic neurons, renal distal convoluted tubule and mammary duct epithelium. Human GATA-3 is 443 amino acids (aa) in length. It contains two GAGA-type Zn finger domains (aa 263-287 and 317-341) and multiple phosphorylation sites. GATAs as a group are known to either activate, or repress, gene expression, maintain transcriptional activity, and regulate gene expression levels. Within this framework, GATA-3 is best known to drive naïve CD4+ T cells into a Th2 phenotype, induce Th2 proliferation, and inhibit Th1 cell development via T-bet repression. Other effects attributed to GATA-33 include the promotion of Th9 and Treg formation, and the inhibition of Th1, TH17 and B cell development. Over aa 135-258, human and mouse GATA-3 share 94% aa sequence identity.

Product Datasheets

Related Research Areas

• gamma delta T Cells
• GATA Transcription Factors
• Group 2 ILCs
• Hematopoietic Stem Cell Transcription Factors and Regulators
• Multiple Sclerosis and Myelin Related Molecules
• Natural Killer T (NKT) Cells
• Pluripotent Stem Cell Transcription Factors
• Signaling Molecules and Transcriptional Regulators of Tfh Cell Differentiation
• Th2 Cells
• Transcription Factors in the Akt Pathway
• Transcriptional Regulators in Allergy and Asthma