Human GDF-15 Quantikine QuicKit ELISA

Catalog #: QK957
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QC282: Human GDF-15 Quantikine QuicKit Control Group 282

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Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human GDF-15 Quantikine QuicKit ELISA Summary

Assay Length

80 minutes

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Urine (10 uL)

Sensitivity

0.947 pg/mL

Assay Range

23.4 - 1,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Recognizes natural and recombinant human GDF-15

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Sample Values

Serum/Plasma/Urine - Samples from apparently healthy volunteers were evaluated for the presence of human GDF-15 in this assay. No medical histories were available for the donors used in this study.

Sample Type	Mean (pg/mL)	Range (pg/mL)	Standard Deviation (pg/mL)
Serum (n=10)	556	204-1146	315
EDTA plasma (n=10)	511	249-1042	281
Heparin plasma (n=10)	539	243-1009	282
Urine (n=4)	4783	3243-8287	2353

Cell Culture Supernates:

HepG2 cells were cultured in MEM NEAA Earle's Salts supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate until nearly confluent. The cell conditioned media was taken off and centrifuged to remove any cells or debris and stored at ≤ -20 °C. An aliquot of the cell culture supernate was removed, assayed for human GDF-15, and measured at 47,216 pg/mL.

JAR cells were cultured in DMEM NEAA Earle's Salts supplemented with 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate until nearly confluent. The cell conditioned media was taken off and centrifuged to remove any cells or debris and stored at ≤ -20 °C. An aliquot of the cell culture supernate was removed, assayed for human GDF-15, and measured at 29,417 pg/mL.

JEG-3 cells were cultured in MEM NEAA Earle's Salts supplemented with 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate until nearly confluent. The cell conditioned media was taken off and centrifuged to remove any cells or debris and stored at ≤ -20 °C. An aliquot of the cell culture supernate was removed, assayed for human GDF-15, and measured at 5651 pg/mL.

Product Summary

The Quantikine™ QuicKit™ Human GDF-15 Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human GDF-15 levels in cell culture supernates, serum, plasma, and urine. It contains CHO cell-expressed recombinant human GDF-15 and antibodies raised against the recombinant protein. Results obtained using natural human GDF-15 showed linear curves that were parallel to the standard curves obtained using the QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human GDF-15.

Precision

Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	20	20	10	10
Mean (pg/mL)	156	882	165	925
Standard Deviation	6.12	58.8	9.49	77.6
CV%	3.9	6.7	5.7	8.4

Recovery

The recovery of human GDF-15 spiked to three levels in samples throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	101	92-113
EDTA Plasma (n=2)	74	66-83
Heparin Plasma (n=2)	77	66-93
Serum (n=2)	77	66-84
Urine (n=2)	104	94-116

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human GDF-15 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human GDF-15 Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GDF-15

Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.

Long Name:

Growth Differentiation Factor 15

Entrez Gene IDs:

9518 (Human); 23886 (Mouse); 29455 (Rat)

Alternate Names:

GDF15; GDF-15; growth differentiation factor 15; growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; MIC-1NSAID-activated gene 1 protein; MIC1Prostate differentiation factor; NAG-1; NAG-1NSAID-regulated gene 1 protein; NSAID (nonsteroidal inflammatory drug)-activated protein 1; PDF; PDFGDF-15; PLAB; PLABNRG-1; Placental bone morphogenetic protein; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta

Related Research Areas

Citation for Human GDF-15 Quantikine QuicKit ELISA

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