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Human GFR alpha-4/GDNF R alpha-4 Antibody

Catalog #: MAB1439
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1439
MAB1439-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GFR alpha-4/GDNF R alpha-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GFR alpha ‑4/GDNF R alpha ‑4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) GFR alpha -1, recombinant rat GFR alpha -1, rhGFR alpha -2, rhGFR alpha -3, or recombinant mouse GFR alpha -4 is observed.
Source
Monoclonal Mouse IgG1 Clone # 215725
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human GFR alpha ‑4/GDNF R alpha ‑4
Asn24-Ser245 (Ser140Cys)(predicted)
Accession # NP_071422
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human GFR alpha ‑4/GDNF R alpha ‑4

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-4/GDNF R alpha-4

GFR alpha -4 is a member of a family of at least four cysteine-rich GPI-linked cell surface proteins that function as ligand binding subunits for the GDNF family (GDNF, neurturin, persephin and artemin). A functional receptor complex is achieved through association with the c-Ret receptor tyrosine kinase. GFR alpha -4 preferentially binds persephin.

Long Name
Glial Cell line-derived Neurotrophic Factor Receptor alpha 4
Entrez Gene IDs
64096 (Human); 14588 (Mouse)
Alternate Names
GDNF family receptor alpha 4; GDNF family receptor alpha-4; GDNF R alpha-4; GDNF receptor alpha-4; GDNFR-alpha-4; GFR alpha4; GFR alpha-4; GFR receptor alpha 4; GFRA4; GFRa-4; GFR-alpha-4; Persephin receptor

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Citation for Human GFR alpha-4/GDNF R alpha-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Expression of Glial-Cell-Line-Derived Neurotrophic Factor Family Ligands in Human Intervertebral Discs
    Authors: Iwasaki, T;Akeda, K;Kawaguchi, K;Yamada, J;Hasegawa, T;Takegami, N;Fujiwara, T;Sudo, A;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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