Human GHRHR Antibody

Catalog #: MAB107352 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB107352-100
MAB107352-SP

Detection of Human GHRHR by Western Blot.

1 Image

All GHRHR Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GHRHR Antibody Summary

Species Reactivity

Human

Specificity

Detects human GHRHR in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1037308

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human GHRHR synthetic peptide
Accession # Q02643

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

4 µg/mL

PC‑3 human prostate cancer cell line, LoVo human colorectal adenocarcinoma cell line, K562 human chronic myelogenous leukemia cell line, and MCF‑7 human breast cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human GHRHR by Western Blot. Western blot shows lysates of PC‑3 human prostate cancer cell line, LoVo human colorectal adenocarcinoma cell line, K562 human chronic myelogenous leukemia cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 4 µg/mL of Mouse Anti-Human GHRHR Monoclonal Antibody (Catalog # MAB107352) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for GHRHR at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GHRHR

GHRHR is a GPCR that binds growth hormone-releasing hormone that is expressed in the pituitary. Significant amounts of GHRHR are also found in the hypothalamus, kidney, and placenta. GHRH regulates the secretion of growth hormone that controls the metabolism and growth of every tissue through its binding with GHRHR. Malfunction in GHRHR signaling is associated with abnormal growth. GHRHR is a therapeutic target against dwarfism, gigantism, lipodystrophy, and certain cancers.

Long Name

Growth Hormone Releasing Hormone Receptor

Entrez Gene IDs

2692 (Human); 14602 (Mouse); 25321 (Rat)

Alternate Names

GHRFR; GHRH receptor; GHRHR; GRF receptor; GRFR; growth hormone releasing hormone receptor; Growth hormone-releasing factor receptor; growth hormone-releasing hormone receptor; IGHD1B; Little