Human GHRHR Antibody

Catalog #: MAB107353 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB107353-100
MAB107353-SP

Detection of GHRHR in Transfected HEK293 (positive) and Wild type (negative) Cells.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GHRHR Antibody Summary

Species Reactivity

Human

Specificity

Detects human GHRHR in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2765A

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human GHRHR synthetic peptide sequences within the first extracellular loop of the protein

Accession # Q02643

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Scientific Data

Immunocytochemistry

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Detection of GHRHR in Transfected HEK293 (positive) and Wild type (negative) Cells. GHRHR was detected in immersion fixed transfected HEK293 (positive) and wild type (negative) cells using Rabbit Anti-Human GHRHR Monoclonal Antibody (Catalog # MAB107353) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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Detection of GHRHR in Human Pituitary. GHRHR was detected in immersion fixed paraffin-embedded sections of human pituitary using Rabbit Anti-Human GHRHR Monoclonal Antibody (Catalog # MAB107353) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GHRHR

GHRHR is a GPCR that binds growth hormone-releasing hormone that is expressed in the pituitary. Significant amounts of GHRHR are also found in the hypothalamus, kidney, and placenta. GHRH regulates the secretion of growth hormone that controls the metabolism and growth of every tissue through its binding with GHRHR. Malfunction in GHRHR signaling is associated with abnormal growth. GHRHR is a therapeutic target against dwarfism, gigantism, lipodystrophy, and certain cancers.

Long Name

Growth Hormone Releasing Hormone Receptor

Entrez Gene IDs

2692 (Human); 14602 (Mouse); 25321 (Rat)

Alternate Names

GHRFR; GHRH receptor; GHRHR; GRF receptor; GRFR; growth hormone releasing hormone receptor; Growth hormone-releasing factor receptor; growth hormone-releasing hormone receptor; IGHD1B; Little