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Human GLP-1R Antibody

Catalog #: MAB28141
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB28141
MAB28141-SP
GLP‑1R in Human Brain.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GLP-1R Antibody Summary

Species Reactivity
Human
Specificity
Detects human GLP‑1R. Stains human GLP-1R-transfected Baf/3 cells but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2A Clone # 419208
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Baf/3 cell line transfected with human GLP-1R
Accession # P43220
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry GLP-1R antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

GLP‑1R in Human Brain. GLP-1R was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using 3 µg/mL Mouse Anti-Human GLP-1R Monoclonal Antibody (Catalog # MAB28141) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLP-1R

Glucagon-Like Peptide-1 Receptor (GLP-1R) is a class B G protein-linked seven transmembrane domain protein that is expressed in pancreas, lung, heart, kidney, hypothalamus, and stomach. GLP-1R functions as a receptor for glucagon-like peptide 1 and regulates insulin and glucagon production.

Long Name
Glucagon-like Peptide 1 Receptor
Entrez Gene IDs
2740 (Human); 14652 (Mouse)
Alternate Names
GLP-1 receptor; GLP1R; GLP-1R; GLP-1-R; glucagon-like peptide 1 receptor; MGC138331

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Citations for Human GLP-1R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Nutrient induced type 2 and chemical induced type 1 experimental diabetes differently modulate gastric GLP-1 receptor expression.
    Authors: Bloch, Olga, Broide, Efrat, Ben-Yehudah, Gilad, Cantrell, Dror, Shirin, Haim, Rapoport, Micha J
    J Diabetes Res, 2015-03-29;2015(0):561353.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. A novel humanized GLP-1 receptor model enables both affinity purification and Cre-LoxP deletion of the receptor.
    Authors: Jun, Lucy S, Showalter, Aaron D, Ali, Nosher, Dai, Feihan, Ma, Wenzhen, Coskun, Tamer, Ficorilli, James V, Wheeler, Michael, Michael, M Dodson, Sloop, Kyle W
    PLoS ONE, 2014-04-02;9(4):e93746.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: IHC

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