Human GluR2 Antibody

Catalog #: MAB11333 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11333-100
MAB11333-SP

Detection of GluR2 in TT (Positive) and THP‑1 (Negative) cells.

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All GluR2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GluR2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GluR2/GRIA2 in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1059613

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human GluR2/GRIA2 containing peptide
Accession # P42262

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of Human Hippocampus

Immunocytochemistry

8-25 µg/mL

Immersion fixed TT Human Medullary Thyroid Cancer Cell Line (Positive) and THP‑1 Human Acute Monocytic Ieukemia Cell Line (Negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of GluR2 in TT (Positive) and THP‑1 (Negative) cells. GluR2 was detected in immersion fixed TT Human Medullary Thyroid Cancer Cells (Positive) and absent in THP‑1 Human Acute Monocytic Leukemia Cells (Negative) using Mouse Anti-Human GluR2 Monoclonal Antibody (Catalog # MAB11333) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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Detection of GluR2 in Human Hippocampus. GluR2 was detected in immersion fixed paraffin-embedded sections of Human Hippocampus using Mouse Anti-Human GluR2 Monoclonal Antibody (Catalog # MAB11333) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GluR2

GluR2 is a receptor for glutamate. Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain. The subunit encoded by this gene (GRIA2) is subject to RNA editing which renders the receptor it becomes part of impermeable to calcium ions. This editing occurs at the Q/R site at a frequency of 100% of GluR2 transcripts in the brain. The main function of this editing is regulation of electrophysiology of the receptor channel. Defective editing of this channel has been linked to several conditions such as ALS, Epilepsy and some human brain tumors. GRIA2 is a diagnostic immunochemical marker for solitary fibrous tumor which distinguishes it from most mimics.

References

Entrez Gene: GRIA glutamate receptor, ionotropic, AMPA 2.
Seeburg PH, Single F, Kuner T, Higuchi M, Sprengel R. "Genetic Manipulation of Key Determinants of Ion Flow in Glutamate Receptor Channels in the Mouse". Brain Res. 2001 Jul; 907(1-2):233.
Cleveland DW, Rothstein JD. "From Charcot to Lou Gehrig: Deciphering Selective Motor Neuron Death in ALS". Nat. Rev. Neurosci. 2001 Nov; 2(11):806.
Maas S, Patt S, Schrey M, Rich A. "Underediting of Glutamate Receptor GluR-B mRNA in Malignant Gliomas". Proc. Natl. Acad. Sci. U.S.A. 2001 Dec; 98(25):14687.
Vivero M, Doyle L.A, Fletcher C.D., Mertens F, Hornick J.L. "GRIA2 is a Novel Diagnostic Marker for Solitary Fibrous Tumour Identified through Gene Expression Profiling". Histopathology. 2014. 65(1):71.

Long Name

Glutamate Receptor 2

Entrez Gene IDs

2891 (Human); 14800 (Mouse); 29627 (Rat)

Alternate Names

AMPA 2; AMPA2; GluA2; GluR2; GLURB; GluR-K2; glutamate receptor, ionotropic, AMPA 2; GRIA2; HBGR2