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Human Glut1 Antibody

Catalog #: MAB1418
14 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1418
MAB1418-SP
Detection of Glut1 in HepG2 Human Cell Line by Flow Cytometry.
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Product Details
Citations (14)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Glut1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Glut1. Stains human Glut1-transfected NS0 cells, but not NS0 control transfectants. Although Human Glut1 Antibody detects Glut1 on the surface of T cells (1, 2), it does not detect it on erythrocytes (5). The reason for this discrepancy is not understood, but may be related to conformational or post-translational modification differences.
Source
Monoclonal Mouse IgG2B Clone # 202915
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Glut1
Met1-Val492
Accession # AAA52571
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Glut1 antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Glut1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Flow Cytometry Detection of Glut1 antibody in Jurkat Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Glut1 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line either (A) untreated or (B) cultured in nutrient-depleted media was stained with Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Immunocytochemistry Glut1 antibody in HepG2 Human Hepatocellular Carcinoma Cell Line by Immunocytochemistry (ICC). View Larger

Glut1 in HepG2 Human Hepatocellular Carcinoma Cell Line. Glut1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glut1

Glut1 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues including brain endothelial cells, CD8+ T cells, and erythrocytes (1‑4). Glut1 is a major glucose transporter that mediates glucose transport across the mammalian blood‑brain barrier.

References
  1. Mueckler, M. et al. 1994, Eur. J. Biochem. 219:713.
  2. Meuckler, M. et al. 1985, Science 229:941.
  3. Jones, K.S. et al. 2006, J. Virol. 8291.
  4. Takenouchi, N. et al. 2007, J. Virol. 1506.
  5. Kinet, S. et al. 2007, Retrovirology 4:31.
Long Name
Glucose Transporter Type 1
Entrez Gene IDs
6513 (Human); 20525 (Mouse)
Alternate Names
Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis); CSE; DYT17; DYT18; DYT9; EIG12; Glucose transporter type 1, erythrocyte/brain; Glut1; GLUT-1; GLUT1DS; HepG2 glucose transporter; HTLVR; Human T-Cell Leukemia Virus (I and II) Receptor; MGC141895; MGC141896; PED; SLC2A1; solute carrier family 2 (facilitated glucose transporter), member 1; Solute Carrier Family 2 Member 1; solute carrier family 2, facilitated glucose transporter member 1

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Citations for Human Glut1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target
    Authors: H Cho, HY Kwon, A Sharma, SH Lee, X Liu, N Miyamoto, JJ Kim, SH Im, NY Kang, YT Chang
    Nature Communications, 2022-10-10;13(1):5974.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Alcohol Impairs Immunometabolism and Promotes Na�ve T Cell Differentiation to Pro-Inflammatory Th1 CD4(+) T Cells
    Authors: McTernan PM, Levitt DE, Welsh DA et al.
    Frontiers in Immunology
  3. Single Cell Mass Cytometry of Non-Small Cell Lung Cancer Cells Reveals Complexity of In vivo And Three-Dimensional Models over the Petri-dish
    Authors: R Alföldi, JÁ Balog, N Faragó, M Halmai, E Kotogány, P Neuperger, LI Nagy, LZ Fehér, GJ Szebeni, LG Puskás
    Cells, 2019-09-16;8(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Rhinovirus induces an anabolic reprogramming in host cell metabolism essential for viral replication
    Authors: GA Gualdoni, KA Mayer, AM Kapsch, K Kreuzberg, A Puck, P Kienzl, F Oberndorfe, K Frühwirth, S Winkler, D Blaas, GJ Zlabinger, J Stöckl
    Proc. Natl. Acad. Sci. U.S.A., 2018-07-09;115(30):E7158-E7165.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Detection and Characterization of CD8+ Autoreactive Memory Stem T Cells in Patients with Type 1 Diabetes
    Authors: D Vignali, E Cantarelli, C Bordignon, A Canu, A Citro, A Annoni, L Piemonti, P Monti
    Diabetes, 2018-03-05;0(0):.
    Species: Human
    Sample Types: Whole Blood
    Applications: IHC
  6. Manipulating Glucose Metabolism during Different Stages of Viral Pathogenesis Can Have either Detrimental or Beneficial Effects
    Authors: SK Varanasi, D Donohoe, U Jaggi, BT Rouse
    J. Immunol., 2017-08-02;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Emerging Role and Characterization of Immunometabolism: Relevance to HIV Pathogenesis, Serious Non-AIDS Events, and a Cure
    J Immunol, 2016-06-01;196(11):4437-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Foxp3-mediated inhibition of Akt inhibits Glut1 (glucose transporter 1) expression in human T regulatory cells.
    Authors: Basu S, Hubbard B, Shevach E
    J Leukoc Biol, 2014-12-09;97(2):279-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection.
    Authors: Palmer C, Ostrowski M, Gouillou M, Tsai L, Yu D, Zhou J, Henstridge D, Maisa A, Hearps A, Lewin S, Landay A, Jaworowski A, McCune J, Crowe S
    AIDS, 2014-01-28;28(3):297-309.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. The chemokine CCL5 regulates glucose uptake and AMP kinase signaling in activated T cells to facilitate chemotaxis.
    Authors: Chan O, Burke J, Gao D, Fish E
    J Biol Chem, 2012-07-10;287(35):29406-16.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Entry of human T-cell leukemia virus type 1 is augmented by heparin sulfate proteoglycans bearing short heparin-like structures.
    Authors: Tanaka A, Jinno-Oue A, Shimizu N
    J. Virol., 2012-01-11;86(6):2959-69.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Role of FDG-PET as a biological marker for predicting the hypoxic status of tongue cancer.
    Authors: Han M, Lee H, Cho K, Kim J, Roh J, Choi S, Nam S, Kim S
    Head Neck, 2011-11-03;34(10):1395-402.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  13. DC-SIGN mediates cell-free infection and transmission of human T-cell lymphotropic virus type 1 by dendritic cells.
    Authors: Jain P, Manuel SL, Khan ZK, Ahuja J, Quann K, Wigdahl B
    J. Virol., 2009-08-19;83(21):10908-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Antiparallel segregation of notch components in the immunological synapse directs reciprocal signaling in allogeneic Th:DC conjugates.
    Authors: Luty WH, Rodeberg D, Parness J, Vyas YM
    J. Immunol., 2007-07-15;179(2):819-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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