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Human Glut3 Antibody

Catalog #: MAB1415
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1415
MAB1415-SP
Detection of Glut3 in NS0 Mouse Cell Line Transfected with Human Glut3 by Flow Cytometry.
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Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Glut3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Glut3. Recognizes human Glut3 expression on human Glut3-transfected NS0 cells, but not the NS0 control transfectants. No cross-reactivity was observed with transfectants expressing human Glut1 or human Glut2.
Source
Monoclonal Mouse IgG2B Clone # 202017
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Glut3 Accession # P11169
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Glut3 antibody in NS0 Mouse Cell Line Transfected with Human Glut3 antibody by Flow Cytometry. View Larger

Detection of Glut3 in NS0 Mouse Cell Line Transfected with Human Glut3 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human Glut3 was stained with Mouse Anti-Human Glut3 Monoclonal Antibody (Catalog # MAB1415, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry Glut3 antibody in SH-SY5Y Human Cell Line by Immunocytochemistry (ICC). View Larger

Glut3 in SH‑SY5Y Human Cell Line. Glut3 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human Glut3 Monoclonal Antibody (Catalog # MAB1415) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: Glut3

Glut3 belongs to the facilitative glucose transporter protein family that comprises 13 members, and is designated SLC2A3 (solute carier family 2, member 3). It is an integral membrane protein with 12 transmembrane domains. Glut3 is the glucose transporter responsible for maintaining an adequate glucose supply to neurons (1, 2). It is also expressed in placenta and articular chondrocytes (3, 4).

References
  1. Vannuci, S.J. et al. (1997) Glia 21:2.
  2. Mueckler, M. et al. (1997) Biochem. Soc. Trans. 25:951.
  3. Illsley, N.P. (2000) Placenta 21:14.
  4. Mobasheri, A. (2002) Cell Biol. Int. 26:297.
Long Name
Glucose Transporter Type 3
Entrez Gene IDs
6515 (Human); 20527 (Mouse)
Alternate Names
FLJ90380; Glut3; GLUT-3; GLUT3Glucose transporter type 3, brain; SLC2A3; solute carrier family 2 (facilitated glucose transporter), member 3; solute carrier family 2, facilitated glucose transporter member 3

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Citations for Human Glut3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Aerobic glycolysis fuels platelet activation: small-molecule modulators of platelet metabolism as anti-thrombotic agents
    Authors: PP Kulkarni, A Tiwari, N Singh, D Gautam, VK Sonkar, V Agarwal, D Dash
    Haematologica, 2018-10-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Rhinovirus induces an anabolic reprogramming in host cell metabolism essential for viral replication
    Authors: GA Gualdoni, KA Mayer, AM Kapsch, K Kreuzberg, A Puck, P Kienzl, F Oberndorfe, K Frühwirth, S Winkler, D Blaas, GJ Zlabinger, J Stöckl
    Proc. Natl. Acad. Sci. U.S.A., 2018-07-09;115(30):E7158-E7165.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. IGF-I increases the recruitment of GLUT4 and GLUT3 glucose transporters on cell surface in hyperthyroidism.
    Authors: Dimitriadis G, Maratou E, Boutati E
    Eur. J. Endocrinol., 2008-03-01;158(3):361-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC

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