Human GM-CSF Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM215
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (2)

Human GM-CSF Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects recombinant human GM-CSF in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 3209
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human GM-CSF
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin
Purity
Protein A or G purified from ascites

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human GM-CSF (Catalog # 215-GM)
under non-reducing conditions only

Human GM-CSF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Human GM‑CSF Antibody (Catalog # MAB615)

Standard: Recombinant Human GM-CSF Protein (Catalog # 215-GM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GM-CSF

Granulocyte macrophage colony stimulating factor (GM-CSF) is a growth factor produced by a variety of lymphoid cells. It binds to receptor heterodimers consisting of a GM-CSF-specific alpha chain and the common beta chain that is shared by the high-affinity receptors for IL-3 and IL-5.

Long Name
Granulocyte Macrophage Growth Factor
Entrez Gene IDs
1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
Alternate Names
colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; CSF-2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; Molgramostim; molgramostin; Sargramostim

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Citations for Human GM-CSF Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010-06-08;56(0):1320.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  2. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  3. Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain.
    Authors: Hirst SJ, Hallsworth MP, Peng Q
    Am. J. Respir. Crit. Care Med., 2002-04-15;165(8):1161-71.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

FAQs

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Reviews for Human GM-CSF Biotinylated Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human GM-CSF Biotinylated Antibody
By Anonymous on 12/10/2021
Application: WB Sample Tested: T cells Species: Human

Human GM-CSF Biotinylated Antibody
By Anonymous on 11/02/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used as a matched pair with MAB615 to develop a sandwich ELISA. Human serum and plasma samples were successfully measured using the pair.