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Human gp130 Antibody

Catalog #: AF-228-NA
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-228-NA
AF-228-SP
Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human gp130 Antibody.
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Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human gp130 Antibody Summary

Species Reactivity
Human
Specificity
Detects human gp130 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant rat gp130, recombinant mouse gp130, recombinant human (rh) IL-1 sRI, rhIL-1 sRII, rhIL-2 sR beta, rhIL-2 sR gamma, rhIL‑4 sR, and rhIL-6 sR is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human gp130
Leu24-Glu619 (Glu619Asp)
Accession # P40189
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.70 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
HeLa human cervical epithelial carcinoma cell line
Neutralization
Measured by its ability to neutralize Oncostatin M/OSM-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 2‑10 µg/mL in the presence of 0.8 ng/mL Recombinant Human Oncostatin M/OSM.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human gp130 Antibody. View Larger

Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human gp130 Antibody. Recombinant Human Oncostatin M/OSM (Catalog # 295-OM) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Oncostatin M/OSM (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human gp130 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-228-NA). The ND50 is typically 2-10 µg/mL.

Western Blot View Larger

Detection of Human gp130 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human gp130 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-228-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for gp130 at approximately 150kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: gp130

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.

Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. At the present time, the in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

References
  1. Narazaki, M. et al. (1993) Blood 82:1120.
  2. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.
Long Name
Glycoprotein 130
Entrez Gene IDs
3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)
Alternate Names
CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha

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Citations for Human gp130 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Empagliflozin Reverses Oxidized LDL-Induced RECK Suppression, Cardiotrophin-1 Expression, MMP Activation, and Human Aortic Smooth Muscle Cell Proliferation and Migration
    Authors: Chandrasekar, B;Mummidi, S;DeMarco, VG;Higashi, Y;
    Mediators of inflammation
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. The Alzheimer's disease-linked protease BACE1 modulates neuronal IL-6 signaling through shedding of the receptor gp130
    Authors: SA Müller, MD Shmueli, X Feng, J Tüshaus, N Schumacher, R Clark, BE Smith, A Chi, S Rose-John, ME Kennedy, SF Lichtentha
    Molecular Neurodegeneration, 2023-02-21;18(1):13.
    Species: Primate
    Sample Types: CSF
    Applications: Western Blot
  3. CD109-GP130 interaction drives glioblastoma stem cell plasticity and chemoresistance through STAT3 activity
    Authors: P Filppu, JT Ramanathan, KJ Granberg, E Gucciardo, H Haapasalo, K Lehti, M Nykter, V Le Joncour, P Laakkonen
    JCI Insight, 2021-05-10;6(9):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Co-Immunoprecipitation, PLA, Western Blot
  4. Phosphoproteomic profiling reveals IL6-mediated paracrine signaling within the Ewing sarcoma family of tumors.
    Authors: Anderson J, Titz B, Akiyama R, Komisopoulou E, Park A, Tap W, Graeber T, Denny C
    Mol Cancer Res, 2014-08-04;12(12):1740-54.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  5. NF-kappaB-induced IL-6 ensures STAT3 activation and tumor aggressiveness in glioblastoma.
    Authors: McFarland B, Hong S, Rajbhandari R, Twitty G, Gray G, Yu H, Benveniste E, Nozell S
    PLoS ONE, 2013-11-11;8(11):e78728.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Activation of the AXL kinase causes resistance to EGFR-targeted therapy in lung cancer.
    Authors: Zhang Z, Lee JC
    Nat. Genet., 2012-07-01;44(8):852-860.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Disturbances in the LIF pathway in the endometrium among women with unexplained infertility.
    Authors: Aghajanova L, Altmae S, Bjuresten K, Hovatta O, Landgren BM, Stavreus-Evers A
    Fertil. Steril., 2008-08-05;91(6):2602-10.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Expression of leukemia inhibitory factor and its receptors is increased during differentiation of human embryonic stem cells.
    Authors: Aghajanova L, Skottman H, Stromberg AM, Inzunza J, Lahesmaa R, Hovatta O
    Fertil. Steril., 2006-09-01;86(4):1193-209.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Breast cancer cells stimulate neutrophils to produce oncostatin M: potential implications for tumor progression.
    Authors: Queen MM, Ryan RE, Holzer RG, Keller-Peck CR, Jorcyk CL
    Cancer Res., 2005-10-01;65(19):8896-904.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling.
    Authors: He W, Gong K, Smith DK, Ip NY
    FEBS Lett., 2005-08-15;579(20):4317-23.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Alterations in Janus kinase (JAK)-signal transducers and activators of transcription (STAT) signaling in patients with end-stage dilated cardiomyopathy.
    Authors: Podewski EK, Hilfiker-Kleiner D, Hilfiker A, Morawietz H, Lichtenberg A, Wollert KC, Drexler H
    Circulation, 2003-02-18;107(6):798-802.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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