Human GPER/GPR30 Antibody Summary
Met1-Ser62
Accession # Q99527
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human GPER by Western Blot. Western blot shows lysates of human brain cortex tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GPER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5534) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for GPER at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Detection of GPER/GPR30 in HEK293 Human Cell Line Transfected with human GPER/GRP30 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human GPER/GRP30 or (B) irrelevant transfectants, and eGFP was stained with Goat Anti-Human GPER/GPR30 Affinity-Purified Polyclonal Antibody (Catalog # AF5534) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). Quadrants were set based on Goat IgG Flow Cytometry Isotype Control (Catalog # AB-108-C, data not shown). View our protocol for Staining Membrane-associated Proteins.
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GPER in Human Brain. GPER was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Goat Anti-Human GPER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5534) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GPER/GPR30
GPER (G-Protein Coupled Estrogen Receptor 1; also GPR30, DRY12 and mER) is a 44 kDa, seven transmembrane (TM) member of the GPR-1 family of molecules. It is ubiquitously expressed, appearing on/in neurons, monocytes and endothelial cells. Its exact location is unclear; it has been described in both the cell membrane and ER, but not by all investigators. Human GPER is 375 amino acids (aa) in length. It contains an N-terminal extracellular region (aa 1‑62), a series of seven TM domains (aa 63‑327), and a C-terminal cytoplasmic tail (aa 328‑375). The initial function attributed to GPER was that of a membrane receptor for estrogen. This is in dispute. There are two potential splice variants for GPER. One shows a deletion of aa 32-49, while a second shows a 99 aa substitution for aa 308‑375. Over aa 1‑62, human GPER shares 57% aa identity with mouse GPER.
Product Datasheets
Citations for Human GPER/GPR30 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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UDP-6-glucose dehydrogenase in hormonally responsive breast cancers
Authors: Price, MJ;Nguyen, AD;Haines, C;Baëta, CD;Byemerwa, J;Murkajee, D;Artham, S;Kumar, V;Lavau, C;Wardell, S;Varghese, S;Goodwin, CR;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
Assessment of G Protein-Coupled Oestrogen Receptor Expression in Normal and Neoplastic Human Tissues Using a Novel Rabbit Monoclonal Antibody
Authors: M Bubb, AL Beyer, P Dasgupta, D Kaemmerer, J Sänger, K Evert, RM Wirtz, S Schulz, A Lupp
International Journal of Molecular Sciences, 2022-05-06;23(9):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Plasma membrane expression of G protein-coupled estrogen receptor (GPER)/G protein-coupled receptor 30 (GPR30) is associated with worse outcome in metachronous contralateral breast cancer
Authors: J Tutzauer, M Sjöström, PO Bendahl, L Rydén, M Fernö, LMF Leeb-Lundb, S Alkner
PLoS ONE, 2020-04-17;15(4):e0231786.
Species: Human
Sample Types: Cell Lysates
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G protein-coupled receptor 30 (GPR30) forms a plasma membrane complex with membrane-associated guanylate kinases (MAGUKs) and protein kinase A-anchoring protein 5 (AKAP5) that constitutively inhibits cAMP production.
Authors: Broselid S, Berg K, Chavera T, Kahn R, Clarke W, Olde B, Leeb-Lundberg L
J Biol Chem, 2014-06-24;289(32):22117-27.
Species: Human
Sample Types: Whole Cells
Applications: IHC-Fr, Western Blot
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