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Human GRP78/HSPA5 Antibody

Catalog #: MAB4846
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4846
MAB4846-SP
Detection of Human GRP78/HSPA5 by Western Blot.
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GRP78/HSPA5 Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human GRP78 at 78 kDa in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 474421
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human GRP78 Met1-Leu654 Accession # P11021
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human GRP78/HSPA5 antibody by Western Blot. View Larger

Detection of Human GRP78/HSPA5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human GRP78/HSPA5 Monoclonal Antibody (Catalog # MAB4846) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GRP78/HSPA5 at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western Detection of Human GRP78/HSPA5 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human GRP78/HSPA5 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for GRP78/HSPA5 at approximately 72 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human GRP78/HSPA5 Monoclonal Antibody (Catalog # MAB4846). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.*Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: GRP78/HSPA5

GRP78 (Glucose-regulated protein 78 kDa; also BiP and HSPA5) is a 72 kDa member of the heat shock protein 70 family of proteins. Intracellularly, GRP78 is an endoplasmic reticulum chaperone that participates in protein folding; extracellularly, it induces IL-10 production from T cells and interacts with Cripto to block TGF-beta signaling. Human GRP78 precursor is 654 amino acids (aa) in length. It contains an 18 aa signal sequence and a 636 aa mature region that shows a hydantoinase A region (aa 145-245) and a C-terminal KDEL motif that is present on intracellular GRP78, but absent on secreted GRP78. There is alternative splicing in the signal sequence (aa 1-10), and multiple single aa substitituion. Over aa 1-654, human GRP78 is more than 97% aa identical to mouse and rat GRP78.

Long Name
75 KD Glucose Regulated Protein
Entrez Gene IDs
3309 (Human); 14828 (Mouse); 25617 (Rat)
Alternate Names
78 kDa glucose-regulated protein; BIP; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; FLJ26106; GRP78; GRP-78; GRP78BIP; Heat shock 70 kDa protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); HSP70-5; HSPA5; Immunoglobulin heavy chain-binding protein; MIF2

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Citations for Human GRP78/HSPA5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Pancreatic INS-1 ?-Cell Response to Thapsigargin and Rotenone: A Comparative Proteomics Analysis Uncovers Key Pathways of ?-Cell Dysfunction
    Authors: Weldemariam MM, Woo J, Zhang Q.
    Chemical Research in Toxicology
  2. MXD3 Promotes Obesity and the Androgen Receptor Signaling Pathway in Gender-Disparity Hepatocarcinogenesis
    Authors: YW Tsai, KS Jeng, MK He, YW Hsieh, HH Lai, CY Lai, CC Huang, CF Chang, CT Huang, GM Her
    Cells, 2021-12-06;10(12):.
    Species: Zebrafish
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Targeting highly pathogenic coronavirus-induced apoptosis reduces viral pathogenesis and disease severity
    Authors: H Chu, H Shuai, Y Hou, X Zhang, L Wen, X Huang, B Hu, D Yang, Y Wang, C Yoon, BH Wong, C Li, X Zhao, VK Poon, JP Cai, KK Wong, ML Yeung, J Zhou, RK Au-Yeung, S Yuan, DY Jin, KH Kok, S Perlman, JF Chan, KY Yuen
    Science Advances, 2021-06-16;7(25):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  4. Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells
    Authors: H Chu, CM Chan, X Zhang, Y Wang, S Yuan, J Zhou, RK Au-Yeung, KH Sze, D Yang, H Shuai, Y Hou, C Li, X Zhao, VK Poon, SP Leung, ML Yeung, J Yan, G Lu, DY Jin, GF Gao, JF Chan, KY Yuen
    J. Biol. Chem., 2018-06-10;0(0):.
    Applications: Western Blot
  5. WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response
    Authors: S Janczar, J Nautiyal, Y Xiao, E Curry, M Sun, E Zanini, AJ Paige, H Gabra
    Cell Death Dis, 2017-07-27;8(7):e2955.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. The endoplasmic reticulum chaperone BiP/GRP78 is important in the structure and function of the human cytomegalovirus assembly compartment.
    Authors: Buchkovich NJ, Maguire TG, Paton AW, Paton JC, Alwine JC
    J. Virol., 2009-09-09;83(22):11421-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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