Human/Hamster ACE-2 Antibody Summary
Gln18-Ser740 (predicted)
Accession # Q9BYF1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human ACE‑2 by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # MAB933) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ACE-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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ACE‑2 in Human Kidney. ACE-2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # MAB933) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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ACE‑2 in Hamster Lung. ACE‑2 was detected in immersion fixed paraffin-embedded sections of hamster lung using Mouse Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB933) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to respiratory bronchioles. Staining was performed our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ACE-2
Angiogensin I Converting Enzyme-2 (ACE-2), also called ACEH (ACE homolog), is a type I transmembrane zinc protease that cleaves angiotensins I and II to produce vasodilatory and anti-proliferative peptides. The balance between ACE-1 and ACE-2 activity is critical for maintaining cardiovascular, renal, and pulmonary function (1). ACE-2 also functions as the cellular uptake receptor for the SARS coronoavirus. Within the extracellular domain, human ACE-2 shares 83% aa sequence identity with mouse and rat ACE-2. Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3). ACE-2 isoforms of 75 kDa and 120 kDa are differentially expressed between lung and kidney, respectively, and a shed soluble form is generated by TACE/ADAM17 mediated cleavage.
- Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
- Crackower, M.A. et al. (2002) Nature 417:822.
- Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
Product Datasheets
Citations for Human/Hamster ACE-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
21
Citations: Showing 1 - 10
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Angiotensin I-Converting Enzyme type 2 expression is increased in pancreatic islets of type 2 diabetic donors
Authors: Fignani, D;Pedace, E;Licata, G;Grieco, G;Aiello, E;Luca, C;Marselli, L;Marchetti, P;Sebastiani, G;Dotta, F;
medRxiv
Species: Human
Sample Types: Whole Tissue
Applications: IHC/IF -
Ocular tropism of SARS-CoV-2 in animal models with retinal inflammation via neuronal invasion following intranasal inoculation
Authors: GU Jeong, HJ Kwon, WH Ng, X Liu, HW Moon, GY Yoon, HJ Shin, IC Lee, ZL Ling, AG Spiteri, NJC King, A Taylor, JS Chae, C Kim, DG Ahn, KD Kim, YB Ryu, SJ Kim, S Mahalingam, YC Kwon
Nature Communications, 2022-12-12;13(1):7675.
Species: Mouse
Sample Types: Whole Tissue
Applications: ICC/IF -
Atypical Unilateral SARS-CoV-2 Pneumonia in a Single Lung Re-Transplanted Patient: A Case Report
Authors: M Furstenber, F Gallais, S Freudenber, R Kessler, MP Chenard, B Renaud-Pic
Transplantation Proceedings, 2022-05-30;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
OM-85 Broncho-Vaxom�, a Bacterial Lysate, Reduces SARS-CoV-2 Binding Proteins on Human Bronchial Epithelial Cells
Authors: L Fang, L Zhou, M Tamm, M Roth
Biomedicines, 2021-10-26;9(11):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Colon adenocarcinoma-derived cells possessing stem cell function can be modulated using renin-angiotensin system inhibitors
Authors: MJ Munro, L Peng, SK Wickremese, ST Tan
PLoS ONE, 2021-08-24;16(8):e0256280.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
ACE2 protein expression within isogenic cell lines is heterogeneous and associated with distinct transcriptomes
Authors: EJ Sherman, BT Emmer
Scientific Reports, 2021-08-05;11(1):15900.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot -
Expression of Components of the Renin-Angiotensin System by Cancer Stem Cells in Renal Clear Cell Carcinoma
Authors: S Siljee, B Milne, HD Brasch, N Bockett, J Patel, PF Davis, A Kennedy-Sm, T Itinteang, ST Tan
Biomolecules, 2021-04-07;11(4):.
Species: Human
Sample Types: Tissue Lysates, Whole Tissue
Applications: IHC-P, Western Blot -
Cancer Stem Cells in Metastatic Head and Neck Cutaneous Squamous Cell Carcinoma Express Components of the Renin-Angiotensin System
Authors: S Siljee, O Buchanan, HD Brasch, N Bockett, J Patel, E Paterson, GL Purdie, PF Davis, T Itinteang, ST Tan
Cells, 2021-01-27;10(2):.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
Comparison of Four SARS-CoV-2 Neutralization Assays
Authors: L Riepler, A Rössler, A Falch, A Volland, W Borena, D von Laer, J Kimpel
Vaccines, 2020-12-28;9(1):.
Species: Human
Sample Types: Cell Lysates
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ACE2 localizes to the respiratory cilia and is not increased by ACE inhibitors or ARBs
Authors: IT Lee, T Nakayama, CT Wu, Y Goltsev, S Jiang, PA Gall, CK Liao, LC Shih, CM Schürch, DR McIlwain, P Chu, NA Borchard, D Zarabanda, SS Dholakia, A Yang, D Kim, H Chen, T Kanie, CD Lin, MH Tsai, KM Phillips, R Kim, JB Overdevest, MA Tyler, CH Yan, CF Lin, YT Lin, DT Bau, GJ Tsay, ZM Patel, YA Tsou, A Tzankov, MS Matter, CJ Tai, TH Yeh, PH Hwang, GP Nolan, JV Nayak, PK Jackson
Nat Commun, 2020-10-28;11(1):5453.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction
Authors: H Katsura, V Sontake, A Tata, Y Kobayashi, CE Edwards, BE Heaton, A Konkimalla, T Asakura, Y Mikami, EJ Fritch, PJ Lee, NS Heaton, RC Boucher, SH Randell, RS Baric, PR Tata
Cell Stem Cell, 2020-10-21;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Heterogeneous expression of the SARS-Coronavirus-2 receptor ACE2 in the human respiratory tract
Authors: ME Ortiz, A Thurman, AA Pezzulo, MR Leidinger, JA Klesney-Ta, PH Karp, P Tan, C Wohlford-L, PB McCray, DK Meyerholz
EBioMedicine, 2020-09-21;60(0):102976.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
ACE2 Protein Landscape in the Head and Neck Region: The Conundrum of SARS-CoV-2 Infection
Authors: G Descamps, L Verset, A Trelcat, C Hopkins, JR Lechien, F Journe, S Saussez
Biology (Basel), 2020-08-18;9(8):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Heterogeneous expression of the SARS-Coronavirus-2 receptor ACE2 in the human respiratory tract
Authors: ME Ortiz Beza, A Thurman, AA Pezzulo, MR Leidinger, JA Klesney-Ta, PH Karp, P Tan, C Wohlford-L, PB McCray, DK Meyerholz
bioRxiv, 2020-08-13;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Robust ACE2 protein expression localizes to the motile cilia of the respiratory tract epithelia and is not increased by ACE inhibitors or angiotensin receptor blockers
Authors: IT Lee, T Nakayama, CT Wu, Y Goltsev, S Jiang, PA Gall, CK Liao, LC Shih, CM Schurch, DR McIlwain, P Chu, NA Borchard, D Zarabanda, SS Dholakia, A Yang, D Kim, T Kanie, CD Lin, MH Tsai, KM Phillips, R Kim, JB Overdevest, MA Tyler, CH Yan, CF Lin, YT Lin, DT Bau, GJ Tsay, ZM Patel, YA Tsou, CJ Tai, TH Yeh, PH Hwang, GP Nolan, JV Nayak, PK Jackson
medRxiv, 2020-05-12;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Angiotensin-converting enzyme 2 is a potential therapeutic target for EGFR-mutant lung adenocarcinoma
Authors: M Yamaguchi, S Hirai, T Sumi, Y Tanaka, M Tada, Y Nishii, T Hasegawa, H Uchida, G Yamada, A Watanabe, H Takahashi, Y Sakuma
Biochem. Biophys. Res. Commun., 2017-04-20;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein.
Authors: Heurich A, Hofmann-Winkler H, Gierer S, Liepold T, Jahn O, Pohlmann S
J Virol, 2013-11-13;88(2):1293-307.
Species: Human
Sample Types: Cell Lysates
Applications: Functional Assay, Western Blot -
Selective and specific regulation of ectodomain shedding of angiotensin-converting enzyme 2 by tumor necrosis factor alpha-converting enzyme.
Authors: Iwata M, Silva Enciso JE, Greenberg BH
Am. J. Physiol., Cell Physiol., 2009-09-16;297(5):C1318-29.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Angiotensin converting enzyme-2 confers endothelial protection and attenuates atherosclerosis.
Authors: Lovren F, Pan Y, Quan A, Teoh H, Wang G, Shukla PC, Levitt KS, Oudit GY, Al-Omran M, Stewart DJ, Slutsky AS, Peterson MD, Backx PH, Penninger JM, Verma S
Am. J. Physiol. Heart Circ. Physiol., 2008-07-25;295(4):H1377-84.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
JNK and PI3k/Akt signaling pathways are required for establishing persistent SARS-CoV infection in Vero E6 cells.
Authors: Mizutani T, Fukushi S, Saijo M, Kurane I, Morikawa S
Biochim. Biophys. Acta, 2005-06-30;1741(1):4-10.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Susceptibility to SARS coronavirus S protein-driven infection correlates with expression of angiotensin converting enzyme 2 and infection can be blocked by soluble receptor.
Authors: Hofmann H, Geier M, Marzi A, Krumbiegel M, Peipp M, Fey GH, Gramberg T, Pohlmann S
Biochem. Biophys. Res. Commun., 2004-07-09;319(4):1216-21.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
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Is Catalog # MAB933 kappa or lambda light chain?
MAB933 is kappa light chain.
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Does Human/Hamster ACE-2 Antibody, Catalog# MAB933, detect mouse ACE2?
We have not evaluated cross-reactivity of this antibody to mouse ACE-2. Human ACE-2 shares 83% amino acid sequence identity with mouse ACE-2. We have one citation on our webpage where researcher reports using MAB933 to detect mouse ACE-2.
Reviews for Human/Hamster ACE-2 Antibody
Average Rating: 4.5 (Based on 4 Reviews)
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IMCD3 cells were transiently transfected with human ACE2. 48 hrs post transfection, the cells were fixed, then stain with the ACE2 antibody (1:250 dilution).
