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Human HB-EGF Antibody

Catalog #: AF-259-NA
40 Citations 1 Review Datasheet / COA / SDS
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AF-259-NA
AF-259-SP
Cell Proliferation Induced by HB‑EGF and Neutralization by Human HB‑EGF Antibody.
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Citations (40)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HB-EGF Antibody Summary

Species Reactivity
Human
Specificity
Detects human HB-EGF in ELISAs and Western blots. In direct ELISAs, less than 1% cross reactivity with recombinant mouse HB-EGF is observed. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) Amphiregulin, rhBetacellulin, rhEpiregulin, and recombinant mouse Epigen is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HB-EGF (R&D Systems, Catalog # 259-HE) Asp63-Leu148 Accession # Q99075
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human HB-EGF (Catalog # 259-HE)
Immunohistochemistry
5-15 µg/mL
See below

Human HB-EGF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human HB‑EGF Biotinylated Antibody (Catalog # BAF259)

Standard: Recombinant Human HB-EGF Protein (Catalog # 259-HE)

Neutralization
Measured by its ability to neutralize HB‑EGF-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. Rubin, J.S. et al. (1991) PNAS 88:415. The Neutralization Dose (ND50) is typically 0.1-0.6 µg/mL in the presence of 20 ng/mL Recombinant Human HB‑EGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by HB‑EGF and Neutralization by Human HB‑EGF Antibody. View Larger

Cell Proliferation Induced by HB‑EGF and Neutralization by Human HB‑EGF Antibody. Recombinant Human HB-EGF (Catalog # 259-HE) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human HB-EGF (20 ng/mL) is neutralized (green line) by increasing concentrations of Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-259-NA). The ND50 is typically 0.1-0.6 µg/mL.

Immunohistochemistry HB-EGF antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

HB-EGF in Human Placenta. HB-EGF was detected in immersion fixed paraffin-embedded sections of human placenta using 15 µg/mL Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑259‑NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry HB-EGF antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

HB-EGF in Human Placenta. HB-EGF was detected in immersion fixed paraffin-embedded sections of human placenta using Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-259-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: HB-EGF

HB-EGF was originally purified based on its heparin-binding property and mitogenic activity on BALB-3T3 fibroblasts from the conditioned medium of the human U-937 histiocytic lymphoma cell line. The natural protein has an apparent molecular mass of 19-23 kDa and exists in multiple forms as a result of heterogenous O-glycosylation and/or N-terminal truncation. In addition to fibroblasts, HB-EGF is also a potent mitogen for keratinocytes and smooth muscle cells but not for capillary endothelial cells. HB-EGF is produced in monocytes and macrophages. In addition, transcription of HB-EGF can be induced in vascular endothelial cells as well as aortic smooth muscle cells (SMC), suggesting that HB-EGF may have an important role in the pathogenesis of atherosclerosis. HB-EGF is a member of the EGF family of mitogens which also include transforming growth factor-alpha (TGF-alpha ), amphiregulin (AR), rat schwanoma-derived growth factor (SDGF), vaccinia growth factor (VGF), and the various ligands for the Her2/ErbB2/neu receptor. All these cytokines are derived from transmembrane precursors that contain one or several EGF structural units in their extracellular domain. Many of these transmembrane precursors are biologically active and seem to play a role in juxtacrine stimulation of adjacent cells. The cDNA for HB-EGF encodes a 204 amino acid residue transmembrane protein that is proteolytically cleaved to generate the soluble HB-EGF. Like EGF, TGF-alpha, and AR; HB-EGF binds to the EGF receptor and activates the receptor tyrosine kinase. HB-EGF is reported to be a more potent SMC mitogen than EGF. It has been suggested that the differential activities found for HB-EGF compared to EGF may be mediated by the heparin-binding properties of HB-EGF. A diphtheria toxin receptor that mediates the endocytosis of the bound toxin has been cloned and found to be identical to the transmembrane HB-EGF precursor.

Long Name
Heparin Binding EGF-like Growth Factor
Entrez Gene IDs
1839 (Human); 15200 (Mouse)
Alternate Names
diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor); Dtr; DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor); Dts; DTSF; HBEGF; HB-EGF; Hegfl; heparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; proheparin-binding EGF-like growth factor

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Citations for Human HB-EGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

40 Citations: Showing 1 - 10
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  1. A vagal reflex evoked by airway closure
    Authors: Schappe, MS;Brinn, PA;Joshi, NR;Greenberg, RS;Min, S;Alabi, AA;Zhang, C;Liberles, SD;
    Nature
    Species: Viral
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. The choroid plexus maintains ventricle volume and adult subventricular zone neuroblast pool, which facilitates post-stroke neurogenesis
    Authors: Taranov, A;Bedolla, A;Iwasawa, E;Brown, FN;Baumgartner, S;Fugate, EM;Levoy, J;Crone, SA;Goto, J;Luo, Y;
    bioRxiv : the preprint server for biology
    Species: Murine polyomavirus strain A3, Viral
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  3. Gintonin-Induced Wound-Healing-Related Responses Involve Epidermal-Growth-Factor-like Effects in Keratinocytes
    Authors: Won, KJ;Lee, R;Choi, SH;Kim, JH;Hwang, SH;Nah, SY;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Bitter taste cells in the ventricular walls of the murine brain regulate glucose homeostasis
    Authors: Q Yu, I Gamayun, P Wartenberg, Q Zhang, S Qiao, S Kusumakshi, S Candlish, V Götz, S Wen, D Das, A Wyatt, V Wahl, F Ectors, K Kattler, D Yildiz, V Prevot, M Schwaninge, G Ternier, P Giacobini, P Ciofi, TD Müller, U Boehm
    Nature Communications, 2023-03-22;14(1):1588.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. A brainstem circuit for nausea suppression
    Authors: C Zhang, LK Vincelette, F Reimann, SD Liberles
    Cell Reports, 2022-06-14;39(11):110953.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Induction of cellular senescence in fibroblasts through beta1-integrin activation by tenascin-C-derived peptide and its protumor effect
    Authors: M Fujita, M Sasada, M Eguchi, T Iyoda, S Okuyama, T Osawa, K Tsuzuranuk, M Sakamoto, Y Hagihara, M Matsumura, S Osada, H Kodama, Y Higami, F Fukai
    American journal of cancer research, 2021-09-15;11(9):4364-4379.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. RAS specific protease induces irreversible growth arrest via p27 in several KRAS mutant colorectal cancer cell lines
    Authors: CK Stubbs, M Biancucci, V Vidimar, KJF Satchell
    Scientific Reports, 2021-09-09;11(1):17925.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Sensory�neuron-derived TAFA4 promotes macrophage tissue repair functions
    Authors: G Hoeffel, G Debroas, A Roger, R Rossignol, J Gouilly, C Laprie, L Chasson, PV Barbon, A Balsamo, A Reynders, A Moqrich, S Ugolini
    Nature, 2021-05-19;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Structural basis of malaria RIFIN binding by LILRB1-containing antibodies
    Authors: Y Chen, K Xu, L Piccoli, M Foglierini, J Tan, W Jin, J Gorman, Y Tsybovsky, B Zhang, B Traore, C Silacci-Fr, C Daubenberg, PD Crompton, R Geiger, F Sallusto, PD Kwong, A Lanzavecch
    Nature, 2021-03-31;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Capture
  10. Morphological remodeling during recovery of the neuromuscular junction from terminal Schwann cell ablation in adult mice
    Authors: RL Hastings, M Mikesh, YI Lee, WJ Thompson
    Sci Rep, 2020-07-07;10(1):11132.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. M2 macrophages reduce the radiosensitivity of head and neck cancer by releasing HB?EGF
    Authors: E Fu, T Liu, S Yu, X Chen, L Song, H Lou, F Ma, S Zhang, S Hussain, J Guo, J Sun, P Yu, X Mao, L Wei
    Oncol. Rep., 2020-05-29;44(2):698-710.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  12. HB-EGF-EGFR Signaling in Bone Marrow Endothelial Cells Mediates Angiogenesis Associated with Multiple Myeloma
    Authors: L Rao, D Giannico, P Leone, AG Solimando, E Maiorano, C Caporusso, L Duda, R Tamma, R Mallamaci, N Susca, A Buonavogli, MC Da Vià, D Ribatti, V De Re, A Vacca, V Racanelli
    Cancers (Basel), 2020-01-10;12(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. HIF-1α is Overexpressed in Odontogenic Keratocyst Suggesting Activation of HIF-1α and NOTCH1 Signaling Pathways
    Authors: NM Miranda da, CT Saab Abe, GP Mitre, RA Mesquita, MSD Silva Kata, AL Ribeiro Ri, RG Jaeger, SM Alves-Júni, AM Smith, JJV Pinheiro
    Cells, 2019-07-17;8(7):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. EGFR is required for Wnt9a-Fzd9b signalling specificity in haematopoietic stem cells
    Authors: S Grainger, N Nguyen, J Richter, J Setayesh, B Lonquich, CH Oon, JM Wozniak, R Barahona, CN Kamei, J Houston, M Carrillo-T, IA Drummond, D Gonzalez, K Willert, D Traver
    Nat. Cell Biol., 2019-05-20;21(6):721-730.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. HIF-1&alpha, NOTCH1, ADAM12, and HB-EGF are overexpressed in mucoepidermoid carcinoma
    Authors: DC Branco, NMM da Costa, CTS Abe, MSDS Kataoka, JJV Pinheiro, SM Alves Júni
    Oral Surg Oral Med Oral Pathol Oral Radiol, 2018-10-05;127(1):e8-e17.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  16. Vascular Endothelial Growth Factor-A165b Restores Normal Glomerular Water Permeability in a Diphtheria-Toxin Mouse Model of Glomerular Injury
    Authors: M Stevens, CR Neal, AHJ Salmon, DO Bates, SJ Harper, S Oltean
    Nephron, 2018-01-26;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. M2 macrophages induce ovarian cancer cell proliferation via a heparin binding epidermal growth factor/matrix metalloproteinase 9 intercellular feedback loop
    Authors: MJ Carroll, A Kapur, M Felder, MS Patankar, PK Kreeger
    Oncotarget, 2016-12-27;7(52):86608-86620.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Osmotic Induction of Angiogenic Growth Factor Expression in Human Retinal Pigment Epithelial Cells.
    Authors: Veltmann M, Hollborn M, Reichenbach A, Wiedemann P, Kohen L, Bringmann A
    PLoS ONE, 2016-01-22;11(1):e0147312.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. MicroRNA-132 enhances transition from inflammation to proliferation during wound healing.
    Authors: Li D, Wang A, Liu X, Meisgen F, Grunler J, Botusan I, Narayanan S, Erikci E, Li X, Blomqvist L, DU L, Pivarcsi A, Sonkoly E, Chowdhury K, Catrina S, Stahle M, Landen N
    J Clin Invest, 2015-06-29;125(8):3008-26.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Specific bone cells produce DLL4 to generate thymus-seeding progenitors from bone marrow.
    Authors: Yu V, Saez B, Cook C, Lotinun S, Pardo-Saganta A, Wang Y, Lymperi S, Ferraro F, Raaijmakers M, Wu J, Zhou L, Rajagopal J, Kronenberg H, Baron R, Scadden D
    J Exp Med, 2015-04-27;212(5):759-74.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  21. A mouse model of otitis media identifies HB-EGF as a mediator of inflammation-induced mucosal proliferation.
    Authors: Suzukawa, Keigo, Tomlin, Julia, Pak, Kwang, Chavez, Eduardo, Kurabi, Arwa, Baird, Andrew, Wasserman, Stephen, Ryan, Allen F
    PLoS ONE, 2014-07-17;9(7):e102739.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  22. Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
    Authors: Smith A, Bowers S, Stratman A, Davis G
    PLoS ONE, 2013-12-31;8(12):e85147.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  23. An EGFR wild type-EGFRvIII-HB-EGF feed-forward loop regulates the activation of EGFRvIII.
    Authors: Li L, Chakraborty S, Yang C, Hatanpaa K, Cipher D, Puliyappadamba V, Rehman A, Jiwani A, Mickey B, Madden C, Raisanen J, Burma S, Saha D, Wang Z, Pingle S, Kesari S, Boothman D, Habib A
    Oncogene, 2013-09-30;33(33):4253-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia.
    Authors: Diaz B, Yuen A, Iizuka S, Higashiyama S, Courtneidge S
    J Cell Biol, 2013-04-15;201(2):279-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  25. Immature doublecortin-positive hippocampal neurons are important for learning but not for remembering.
    Authors: Vukovic, Jana, Borlikova, Gilyana, Ruitenberg, Marc J, Robinson, Gregory, Sullivan, Robert K, Walker, Tara L, Bartlett, Perry F
    J Neurosci, 2013-04-10;33(15):6603-13.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  26. Heparin-binding epidermal growth factor-like growth factor eliminates constraints on activated Kras to promote rapid onset of pancreatic neoplasia.
    Authors: Ray, K C, Moss, M E, Franklin, J L, Weaver, C J, Higginbotham, J, Song, Y, Revetta, F L, Blaine, S A, Bridges, L R, Guess, K E, Coffey, R J, Crawford, H C, Washington, M K, Means, A L
    Oncogene, 2013-02-04;33(7):823-31.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  27. Heparin binding epidermal growth factor in renal ischaemia/reperfusion injury.
    Authors: Mulder GM, Nijboer WN, Seelen MA, Sandovici M, Bos EM, Melenhorst WB, Trzpis M, Kloosterhuis NJ, Visser L, Henning RH, Leuvenink HG, Ploeg RJ, Sunnarborg SW, van Goor H
    J. Pathol., 2010-06-01;221(2):183-92.
    Species: Human, Mouse, Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  28. Amphiregulin carboxy-terminal domain is required for autocrine keratinocyte growth.
    Authors: Stoll SW, Johnson JL, Li Y, Rittie L, Elder JT
    J. Invest. Dermatol., 2010-04-29;130(8):2031-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  29. HER family receptor abnormalities in lung cancer brain metastases and corresponding primary tumors.
    Authors: Sun M, Behrens C, Feng L, Ozburn N, Tang X, Yin G, Komaki R, Varella-Garcia M, Hong WK, Aldape KD, Wistuba II
    Clin. Cancer Res., 2009-07-21;15(15):4829-37.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  30. Wounding-induced synthesis of hyaluronic acid in organotypic epidermal cultures requires the release of heparin-binding egf and activation of the EGFR.
    Authors: Monslow J, Sato N, Mack JA, Maytin EV
    J. Invest. Dermatol., 2009-02-19;129(8):2046-58.
    Species: Human, Rat
    Sample Types: Cell Lysates, Recombinant Protein
    Applications: Western Blot
  31. Expression and role of EGFR ligands induced in airway cells by PM2.5 and its components.
    Authors: Rumelhard M, Ramgolam K, Hamel R, Marano F, Baeza-Squiban A
    Eur. Respir. J., 2007-09-05;30(6):1064-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  32. EGFR signaling is required for TGF-beta 1 mediated COX-2 induction in human bronchial epithelial cells.
    Authors: Liu M, Yang SC, Sharma S, Luo J, Cui X, Peebles KA, Huang M, Sato M, Ramirez RD, Shay JW, Minna JD, Dubinett SM
    Am. J. Respir. Cell Mol. Biol., 2007-06-28;37(5):578-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  33. Ras activation in response to phorbol ester proceeds independently of the EGFR via an unconventional nucleotide-exchange factor system in COS-7 cells.
    Authors: Rubio I, Rennert K, Wittig U, Beer K, Durst M, Stang SL, Stone J, Wetzker R
    Biochem. J., 2006-09-01;398(2):243-56.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Types: Cell Lysates
    Applications: Western Blot
  34. Role of epidermal growth factor receptor transactivation in alpha1B-adrenoceptor phosphorylation.
    Authors: Casas-Gonzalez P, Garcia-Sainz JA
    Eur. J. Pharmacol., 2006-05-27;542(1):31-6.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  35. Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
    Authors: Li Y, Fan J, Chen M, Li W, Woodley DT
    J. Invest. Dermatol., 2006-05-11;126(9):2096-105.
    Species: Human
    Sample Types: Serum
    Applications: Neutralization
  36. Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor.
    Authors: Armant DR, Kilburn BA, Petkova A, Edwin SS, Duniec-Dmuchowski ZM, Edwards HJ, Romero R, Leach RE
    Development, 2006-01-11;133(4):751-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  37. Expression of heparin-binding epidermal growth factor-like growth factor and its receptors in the human fallopian tube and endometrium after treatment with mifepristone.
    Authors: Sun XX, Gemzell-Danielsson K, Li HZ, Stabi B, Stavreus-Evers A
    Fertil. Steril., 2006-01-01;85(1):171-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  38. Trafficking of nuclear heparin-binding epidermal growth factor-like growth factor into an epidermal growth factor receptor-dependent autocrine loop in response to oxidative stress.
    Authors: Kim J, Adam RM, Freeman MR
    Cancer Res., 2005-09-15;65(18):8242-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  39. Autocrine extracellular signal-regulated kinase (ERK) activation in normal human keratinocytes: metalloproteinase-mediated release of amphiregulin triggers signaling from ErbB1 to ERK.
    Authors: Kansra S, Johnson JL
    Mol. Biol. Cell, 2004-07-14;15(9):4299-309.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  40. Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype.
    Authors: Leach RE, Kilburn B, Wang J, Liu Z, Romero R, Armant DR
    Dev. Biol., 2004-02-15;266(2):223-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization

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Human HB-EGF Antibody
By Tamar Licht on 11/30/2017
Application: ICC/IF Sample Tested: Adult brain Species: Mouse- transgenic inducible DTR

Perfect to detect expression of transgenic human diphtheria toxin receptor in mouse.
Here I show POMC-Cre line crossed to iDTR line. Mouse hypothalamus is presented. HB-EFG in green.

Immunocytochemistry/Immunofluorescence Human HB-EGF Antibody AF-259-NA