Human HGF Antibody

Name: Human HGF Antibody
Brand: R&D Systems
SKU: MAB694
Rating: 5 (2 reviews)

Catalog #: MAB694
7 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB694-SP
MAB694-100
MAB694-500

All HGF Antibodies

Product Details

Citations (7)

FAQs

Supplemental Products

Reviews (2)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HGF Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human (rh)HGF in direct ELISAs and Western blots. In direct ELISAs, less than 0.25% cross-reactivity was observed with rhMSP.

Source

Monoclonal Mouse IgG₁ Clone # 24516

Purification

Protein A or G purified from ascites

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HGF
Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human HGF (Catalog # 294-HG)
under non-reducing conditions only

Human HGF Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human HGF Biotinylated Antibody (Catalog # BAF294)

Standard: Recombinant Human HGF Protein (Catalog # 294-HG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HGF

HGF, also known as Hepatopoietin A and Scatter Factor, is a liver- and fibroblast-derived soluble protein. It interacts with HGF R to promote mitogenesis and morphogenesis of endothelial and epithelial cells.

Long Name

Hepatocyte Growth Factor

Entrez Gene IDs

3082 (Human); 15234 (Mouse); 24446 (Rat); 403441 (Canine); 102133907 (Cynomolgus Monkey); 493705 (Feline)

Alternate Names

deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human HGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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In vitro differentiated human CD4+ T cells produce hepatocyte growth factor
Authors: Shayne Lavondua Ford, Terkild Brink Buus, Claudia Nastasi, Carsten Geisler, Charlotte Menné Bonefeld, Niels Ødum et al.
Frontiers in Immunology
Novel antibody reagents for characterization of drug- and tumor microenvironment-induced changes in epithelial-mesenchymal transition and cancer stem cells
Authors: T Navas, TD Pfister, S Colantonio, A Aziz, L Dieckman, RG Saul, J Kaczmarczy, S Borgel, SY Alcoser, MG Hollingshe, YH Lee, DP Bottaro, T Hiltke, G Whiteley, N Takebe, RJ Kinders, RE Parchment, JE Tomaszewsk, JH Doroshow
PLoS ONE, 2018-06-21;13(6):e0199361.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development (Capture)
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008-04-19;7(6):2406-14.
Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development
Hepatocyte growth factor in polymorphonuclear leukocytes is increased in patients with systemic inflammatory response syndrome.
Authors: Matsushima A, Ogura H, Koh T, Fujita K, Yoshiya K, Sumi Y, Hosotsubo H, Kuwagata Y, Tanaka H, Shimazu T, Sugimoto H
J Trauma, 2004-02-01;56(2):259-64.
Species: Human
Sample Types: Cell Culture Supernates, Whole Cells
Applications: Flow Cytometry, ICC, Western Blot
Combination therapy with pazopanib and tivantinib modulates VEGF and c-MET levels in refractory advanced solid tumors
Authors: Shivaani Kummar, Apurva K. Srivastava, Tony Navas, Fabiola Cecchi, Young H. Lee, Donald P. Bottaro et al.
Investigational New Drugs
Use of a Human Artificial Chromosome for Delivering Trophic Factors in a Rodent Model of Amyotrophic Lateral Sclerosis
Authors: Yasuhiro Watanabe, Yasuhiro Kazuki, Kanako Kazuki, Mitsutaka Ebiki, Mami Nakanishi, Kazuomi Nakamura et al.
Molecular Therapy - Nucleic Acids
Aligned Nanofibrillar Scaffolds for Controlled Delivery of Modified mRNA
Authors: Tatiana S. Zaitseva, Cynthia Alcazar, Maedeh Zamani, Luqia Hou, Steve Sawamura, Eduard Yakubov et al.
Tissue Engineering Part A