Human HM74A/PUMA-G/GPR109A Antibody

Catalog #: MAB27601 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB27601-100
MAB27601-SP

HM74A/PUMA-G/GPR109A in Human Spleen Tissue.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HM74A/PUMA-G/GPR109A Antibody Summary

Species Reactivity

Human

Specificity

Detects human HM74A/PUMA-G/GPR109A in direct ELISAs.

Source

Monoclonal Rat IgG_2B Clone # 245115

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human HM74A/PUMA-G/GPR109A
Met1-Pro363
Accession # Q8TDS4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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HM74A/PUMA-G/GPR109A in Human Spleen Tissue. HM74A/PUMA-G/GPR109A was detected in immersion fixed paraffin-embedded sections of human spleen tissue using Rat Anti-Human HM74A/PUMA-G/GPR109A Monoclonal Antibody (Catalog # MAB27601) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HM74A/PUMA-G/GPR109A

HM74A, also known as GPR109A, is a high affinity G-protein coupled receptor for nicotinic acid. It is primarily expressed in adipose tissue and spleen.

Long Name

G Protein-coupled Receptor HM74A/G Protein-coupled Receptor 109A

Entrez Gene IDs

338442 (Human); 80885 (Mouse)

Alternate Names

GPR109A; HCA2; HCAR2; HM74A; hydroxycarboxylic acid receptor 2; Niacr1; Nicotinic Acid Receptor; PUMAG