Human ICAT Antibody

Catalog #: AF3016 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3016
AF3016-SP

All ICAT Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ICAT Antibody Summary

Species Reactivity

Human

Specificity

Detects human ICAT in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ICAT
Met1-Gln81
Accession # Q9NSA3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human ICAT

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAT

Human ICAT is a physiological inhibitor of the beta -Catenin/Wnt signaling pathway. It is 9 kDa in size and found in both cytosol and nucleus. The molecule contains an alpha ‑helical N-terminal region (aa 9‑54) and an acidic, extended C-terminal region (aa 55‑78). The alpha -helical region binds to beta -Catenin, displacing CBP/p300, while the acidic C-terminus interacts with the beta -Catenin N-terminus, blocking Tcf interaction. Human ICAT shares 97% aa sequence identity with mouse and bovine ICAT, respectively.

Long Name

Beta-Catenin-Interacting Protein

Entrez Gene IDs

56998 (Human)

Alternate Names

beta-catenin-interacting protein ICAT; catenin, beta interacting protein 1; CTNNBIP1; ICAT; ICATbeta-interacting protein 1; Inhibitor of beta-catenin and Tcf-4