Human IFN-alpha 2 Quantikine HS ELISA Kit

Catalog #: HSIFNA2 Datasheet / COA / SDS

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HSIFNA2

R&D Systems Quantikine HS High Sensitivity Colorimetric Sandwich ELISAs

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IFN-alpha 2 Quantikine HS ELISA Kit Summary

Assay Length

4.0 hours

Sensitivity

0.107 pg/mL

Assay Range

0.8 - 50 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human IFN-alpha 2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine™ HS Human IFN-alpha 2 Immunoassay is a 4.0 hour solid phase ELISA designed to measure human IFN-alpha 2 (IFN-alpha 2a, IFN-alpha 2b, IFN-alpha 2c) in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant human IFN-alpha 2a and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IFN-alpha 2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-alpha 2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to asses inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	2.38	15.1	32.3	2.65	15.5	29.7
Standard Deviation	0.082	0.686	1.36	0.15	0.965	1.69
CV%	3.4	4.5	4.2	5.7	6.2	5.7

Recovery

The recovery of human IFN-alpha 2 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	101	90-108
EDTA Plasma (n=4)	89	75-101
Heparin Plasma (n=4)	86	75-92
Serum (n=4)	86	75-99

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IFN-alpha 2 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-alpha 2/IFNA2

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Long Name:

Interferon alpha 2

Entrez Gene IDs:

3440 (Human); 15965 (Mouse); 298213 (Rat)

Alternate Names:

alpha-2a interferon; Ifa2; IFNA; IFNA2; IFNA2a; IFNA2b; IFNA2c; IFNAA; IFNalpha 2; IFN-alpha 2; IFN-alpha-2; IFN-alphaA; INFA2; interferon alpha 2b; interferon alpha A; interferon alpha-2; Interferon alpha-A; interferon, alpha 2; LeIF A; MGC125764; MGC125765