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Human IFN-gamma Antibody

Catalog #: AF-285-NA
13 Citations 3 Reviews Datasheet / COA / SDS
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AF-285-NA
AF-285-SP
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IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma Antibody.
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Citations (13)
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Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IFN-gamma Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN-gamma in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IFN-gamma
Met1-Gln144
Accession # Q14609
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IFN-gamma (Catalog # 285-IF)
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IFN-gamma inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 0.0300 - 0.300 µg/mL in the presence of 5.00 ng/mL Recombinant Human IFN-gamma.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma Antibody. View Larger

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma Antibody. Recombinant Human IFN-gamma (285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-gamma (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA). The ND50 is typically 0.0300 ‑ 0.300 µg/mL.

Immunocytochemistry IFN‑? antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IFN‑ gamma in Human PBMCs. IFN-gamma was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red); (NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma

Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. Glycosylation of IFN-gamma at sites Asn25 and Asn97 is critical for protease resistance. It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.

Long Name
Interferon gamma
Entrez Gene IDs
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

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Citations for Human IFN-gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Longitudinal analyses reveal distinct immune response landscapes in lung and intestinal tissues from SARS-CoV-2-infected rhesus macaques
    Authors: H Zheng, Y Chen, J Li, H Li, X Zhao, J Li, F Yang, Y Li, C Liu, L Qin, Y Zuo, Q Zhang, Z He, H Shi, Q Li, L Liu
    Cell Reports, 2022-05-08;39(8):110864.
    Species: Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC
  2. Killer cell proteases can target viral immediate-early proteins to control human cytomegalovirus infection in a noncytotoxic manner
    Authors: L Shan, S Li, J Meeldijk, B Blijenberg, A Hendriks, KJWM van Boxtel, SPH van den Be, IJ Groves, M Potts, A Svrlanska, T Stamminger, MR Wills, N Bovenschen
    PLoS Pathog., 2020-04-13;16(4):e1008426.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Engineered triple inhibitory receptor resistance improves anti-tumor CAR-T cell performance via CD56
    Authors: F Zou, L Lu, J Liu, B Xia, W Zhang, Q Hu, W Liu, Y Zhang, Y Lin, S Jing, M Huang, B Huang, B Liu, H Zhang
    Nat Commun, 2019-09-11;10(1):4109.
    Species: Xenograft
    Sample Types: Whole Tissue
    Applications: CAR-T (IHC-Fr)
  4. The Therapeutic Effect of Pamidronate on Lethal Avian Influenza A H7N9 Virus Infected Humanized Mice.
    Authors: Zheng J, Wu W, Liu Y, Xiang Z, Liu M, Chan K, Lau S, Lam K, To K, Chan J, Li L, Chen H, Lau Y, Yuen K, Tu W
    PLoS ONE, 2015-08-18;10(8):e0135999.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Natural killer cell-produced IFN-gamma and TNF-alpha induce target cell cytolysis through up-regulation of ICAM-1.
    Authors: Wang R, Jaw JJ, Jaw J, Stutzman NC, Stutzman N, Zou Z, Sun PD
    J Leukoc Biol, 2011-11-01;91(2):299-309.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-gamma.
    Authors: Knoblach T, Grandel B, Seiler J, Nevels M, Paulus C
    PLoS Pathog., 2011-04-14;7(4):e1002016.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Immunoproteasome expression is induced in mesial temporal lobe epilepsy.
    Authors: Mishto M, Ligorio C, Bellavista E, Martucci M, Santoro A, Giulioni M, Marucci G, Franceschi C
    Biochem. Biophys. Res. Commun., 2011-03-30;408(1):65-70.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
    Authors: Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ
    Blood, 2010-06-10;116(12):2061-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients.
    Authors: Heo YJ, Joo YB, Oh HJ, Park MK, Heo YM, Cho ML, Kwok SK, Ju JH, Park KS, Cho SG, Park SH, Kim HY, Min JK
    Immunol. Lett., 2009-11-04;127(2):150-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Evolving immunosuppressive microenvironment during human cervical carcinogenesis.
    Authors: Kobayashi A, Weinberg V, Darragh T, Smith-McCune K
    Mucosal Immunol, 2008-07-02;1(5):412-20.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Upregulated IL-23 and IL-17 in Behcet patients with active uveitis.
    Authors: Chi W, Zhu X, Yang P, Liu X, Lin X, Zhou H, Huang X, Kijlstra A
    Invest. Ophthalmol. Vis. Sci., 2008-07-01;49(7):3058-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
    Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
    Eur. J. Immunol., 2008-04-01;38(4):1012-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Cytokine profile of the rheumatoid nodule suggests that it is a Th1 granuloma.
    Authors: Hessian PA, Highton J, Kean A, Sun CK, Chin M
    Arthritis Rheum., 2003-02-01;48(2):334-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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Reviews for Human IFN-gamma Antibody

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Human IFN-gamma Antibody
By Stephanie Zandee on 04/13/2018
Application: IHC Sample Tested: Adult brain Species: Human

Published in https://www.ncbi.nlm.nih.gov/pubmed/28169287
Used at 10ug/ml.
Briefly, frozen brain sections were fixed in 4% PFA (Fisher Scientific), followed by antigen retrieval using heating in acid citric buffer (Vector, Burlingame, CA, USA). Endogenous avidin-biotin was blocked for 15 min (Vector). Sections were incubated with 10% horse serum in PBS (Biosera, Boussens, France) and Fc Receptor Blocking Solution was added (Human TruStain FcX Biolegend,
London, UK). Primary antibody was added overnight at 4 °C and detected with donkey anti-goat-biotin (ab6578, Abcam), followed by streptavidin-alkaline phosphatase (SA-5100, Vector) and visualised with the Vector Blue Alkaline Phosphatase Substrate Kit III (Vector).

Immunohistochemistry Human IFN-gamma Antibody AF-285-NA
Human IFN-gamma Antibody
By Becker Law on 10/01/2017
Application: ICC/IF Sample Tested: Kidney tissue (cortex) Species: Human
Immunocytochemistry/Immunofluorescence Human IFN-gamma Antibody AF-285-NA
Human IFN-gamma Antibody
By Shijun Yan on 06/29/2016
Application: ELISA Sample Tested: activated mouse CD8 T cell Species: Mouse