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Human IFN-gamma  R2 Antibody

Catalog #: AF773
4 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF773
AF773-SP
Detection of IFN‑ gamma R2 in human blood‑derived granulocytes
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Product Details
Citations (4)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IFN-gamma  R2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN-gamma  R2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IFN-gamma R2
Ala22-Gln247
Accession # P38484
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IFN-gamma  R2
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize IFN‑ gamma  R2-mediated inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line [Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129]. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 2 ng/mL Recombinant Human IFN‑ gamma.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IFN-gamma R2 antibody in human blood-derived granulocytes View Larger

Detection of IFN‑ gamma R2 in human blood‑derived granulocytes Blood-derived granulocytes were stained with Goat Anti-Human IFN-gamma R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF773, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Neutralization IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma R2 Antibody. View Larger

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma R2 Antibody. Recombinant Human IFN-gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IFN-gamma (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN-gamma R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF773). The ND50 is typically 0.5-2.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma R2

Interferon gamma Receptor 2 (IFN-gamma R2) is a type II transmembrane protein that associates with IFN-gamma R1 to form a high affinity receptor complex for IFN-gamma. IFN‑ gamma  R2 is required for signal transduction but does not bind IFN-gamma by itself.

Long Name
Interferon gamma Receptor 2
Entrez Gene IDs
3460 (Human); 15980 (Mouse)
Alternate Names
AF-1; AF-1interferon-gamma receptor beta chain precursor10IFNGT1Interferon gamma receptor accessory factor 1; IFGR2; IFN-gamma R2; IFN-gamma receptor 2; IFNgammaR2; IFN-gamma-R2; IFNGR2; IFN-gR2; IFNGT1; interferon gamma receptor 2 (interferon gamma transducer 1); interferon gamma receptor 2; interferon gamma receptor accessory factor-1; interferon gamma receptor beta chain; Interferon gamma transducer 1

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Citations for Human IFN-gamma  R2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity
    Authors: J Joung, PC Kirchgatte, A Singh, JH Cho, SP Nety, RC Larson, RK Macrae, R Deasy, YY Tseng, MV Maus, F Zhang
    Nature Communications, 2022-03-25;13(1):1606.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Glycosylation-Dependent IFN-?R Partitioning in Lipid and Actin Nanodomains Is Critical for JAK Activation
    Authors: CM Blouin, Y Hamon, P Gonnord, C Boularan, J Kagan, C Viaris de, R Ruez, S Mailfert, N Bertaux, D Loew, C Wunder, L Johannes, G Vogt, FX Contreras, D Marguet, JL Casanova, C Galès, HT He, C Lamaze
    Cell, 2016-08-04;166(4):920-34.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Trisomy 21 consistently activates the interferon response
    Elife, 2016-07-29;5(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Immunologic analysis of HIV-uninfected Taiwanese children with BCG-induced disease.
    Authors: Lee WI, Liang FC, Huang JL, Jaing TH, Wang CH, Lin TY, Huang YC, Huang WL, Jou R, Hsieh MY, Chia JH, Wu TL
    J. Clin. Immunol., 2008-12-13;29(3):319-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human IFN-gamma  R2 Antibody

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Human IFN-gamma  R2 Antibody
By Anonymous on 07/16/2018
Application: WB Sample Tested: Colon cancer cell line Species: Human

Cancer cells as indicated were treated with or without INF-g (10ng/ml) for 24h. Total cell lysates were subjected to western blot. PVDF membrane were probed with 1mm/ml Human Human IFN-gamma R2 Antibody (AF773). A specific band was detected for IFN-gamma R2 at approximately 40kDa. This experiment was conducted under reducing conditions

Western Blot Human IFN-gamma  R2 Antibody AF773