Human IGF-1 R/IGF1R F(ab')2 (Research Grade Teprotumumab Biosimilar) Antibody

Catalog #: MAB11053-FAB2 Datasheet / COA / SDS

Recombinant Monoclonal Antibody For Research Use Only

Catalog #	Availability	Size / Price	Qty
MAB11053-FAB2-100
MAB11053-FAB2-SP

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Human IGF-1 R/IGF1R F(ab')2 (Research Grade Teprotumumab Biosimilar) Antibody Summary

Species Reactivity

Human

Specificity

Detects human IGF-1/IGF-1R in direct ELISAs.

Source

Recombinant Monoclonal Human IgG₁ Clone # Hu14F2

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human IGF-1 R/IGF1R

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

MCF-7 human breast cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IGF-1 R/IGF1R in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Human Anti-Human IGF-1 R/IGF1R (Teprotumumab) Monoclonal Antibody (Catalog # MAB11053-FAB2, filled histogram) or Human IgG control antibody (1-001-A, open histogram) followed by anti-Human IgG APC-conjugated secondary antibody (F0135). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I R/IGF1R

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.

References

Rechler, M.M. and S.P. Nissley (1990) in Insulin-Like Growth Factors. Sporn, M.B. and A.B. Roberts (eds): Peptide Growth Factors and Their Receptors I, New York: Springer-Verlag, p. 263.

Long Name

Insulin-like Growth Factor I Receptor

Entrez Gene IDs

3480 (Human)

Alternate Names

CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2