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Human IGF-II R/IGF2R Antibody

Catalog #: AF2447
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AF2447
AF2447-SP
Detection of IGF-II R/IGF2R in Human Monocytes by Flow Cytometry.
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Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IGF-II R/IGF2R Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGF-II R/IGF2R in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IGF-II R/IGF2R
Ser1510-Phe2108
Accession # P11717
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IGF-II R/IGF2R (Catalog # 2447-GR)
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.5-2.5 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human IGF-II/IGF2 (Catalog # 292-G2) to immobilized Recombinant Human IGF-II R/IGF2R (Catalog # 2447-GR) coated at 2 µg/mL (100 µL/well). At 20 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IGF-II R/IGF2R antibody in Human Monocytes antibody by Flow Cytometry. View Larger

Detection of IGF-II R/IGF2R in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Goat Anti-Human IGF-II R/IGF2R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2447, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Immunocytochemistry IGF-II R/IGF2R antibody in A172 Human Cell Line by Immunocytochemistry (ICC). View Larger

IGF-II R/IGF2R in A172 Human Cell Line. IGF-II R/IGF2R was detected in immersion fixed A172 human glioblastoma cell line using Goat Anti-Human IGF-II R/IGF2R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2447) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry IGF-II R/IGF2R antibody in A549 Human Cell Line by Immunocytochemistry (ICC). View Larger

IGF-II R/IGF2R in A549 Human Cell Line. IGF-II R/IGF2R was detected in immersion fixed A549 human lung carcinoma cell line using Goat Anti-Human IGF-II R/IGF2R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2447) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry IGF-II R/IGF2R R antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

IGF-II R/IGF2R R in Human Placenta. IGF-II R/IGF2R was detected in immersion fixed paraffin-embedded sections of human placenta using 10 µg/mL Goat Anti-Human IGF-II R/IGF2R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2447) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-II R/IGF2R

The type 2 insulin-like growth factor receptor (also known as cation-independent mannose-6 phosphate receptor/CI-MPR) is a 300 kDa member of the P-type lectin family of molecules. P-type lectins generate functional eukaryotic lysosomes by binding and sorting lysosomal enzymes expressing phosphorylated mannose residues (M6P) (1-3). IGF-II R is a type I transmembrane glycoprotein that contains a 2,264 amino acid (aa) extracellular region, a 23 aa transmembrane segment and a 124 aa cytoplasmic tail (4, 5). The extracellular region consists of 15 contiguous “binding” repeats of about 150 aa each. The odd-numbered repeats interact with “ligands” while the even-numbered repeats likely generate a nondisulfide homodimer in the membrane (1). Repeat #11 binds IGF-II, while repeats 3 and 9 bind mannose-6 phosphate; repeat #13 contains a fibronectin type II motif and assists in IGF-II binding (1, 2). In the extracellular region of IGF-II R expressed by R&D Systems (600 aa’s), human IGF-II R is 85% aa identical to both mouse and bovine IGF-II R. This expressed region includes binding repeats #11, 12, and 13. In addition to IGF-II, CI-MPR/IGF-II R binds non-M6P containing ligands such as retinoic acid, urokinase-type plasminogen-activator receptor and plasminogen, plus M6P‑containing molecules such as lysosomal enzymes, TGF-beta 1 precursor, proliferin, LIF, CD26, herpes simplex glycoprotein D, and granzymes A and B (2, 6). IGF-II R regulates many diverse biological functions that range from intracellular trafficking to the internalization of extracellular factors and modulation of cellular responses. It delivers newly synthesized M6P-tagged lysosomal enzymes from the trans-golgi network to endosomes, and facilitates the clearance of extracellular lysosomal and matrix degrading enzymes by internalization into clathrin-coated vesicles and delivery into endosomes. With respect to IGF-II biology, it would appear that IGF-II R is principally a regulator of local IGF-II levels, targeting IGF-II for destruction in lysosomes (2). However, some evidence suggests the receptor will signal via G‑proteins, an effect that has yet to be conclusively shown (6).

References
  1. Ghosh, P. et al. (2003) Nat. Rev. Mol. Cell. Biol. 4:202.
  2. Dahms, N.M. and M.K. Hancock (2002) Biochim. Biophys. Acta. 1572:317.
  3. Zaina, S. and J. Nilsson (2003) Curr. Opin. Lipidol. 14:483.
  4. Morgan, D.O. et al. (1987) Nature 329:301.
  5. Oshima, A. et al. (1988) J. Biol. Chem. 263:2553.
  6. Hawkes, C. and S. Kar (2004) Brain Res. Rev. 44:117.
Long Name
Insulin-like Growth Factor II Receptor
Entrez Gene IDs
3482 (Human)
Alternate Names
300 kDa mannose 6-phosphate receptor; cation-independent mannose-6 phosphate receptor; cation-independent mannose-6-phosphate receptor; CD222 antigen; CD222; CI Man-6-P receptor; CIMPR; CI-MPR; IGF2R; IGF-II R; IGF-II receptor; IGFIIR; IGF-IIR; insulin-like growth factor 2 receptorM6P/IGF2R; Insulin-like growth factor II receptor; M6P/IGF2 receptor; M6PR; M6P-R; MPR 300; MPR1; MPRI; MPRIM6PR

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Citations for Human IGF-II R/IGF2R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Blockade of IGF2R improves muscle regeneration and ameliorates Duchenne muscular dystrophy
    Authors: P Bella, A Farini, S Banfi, D Parolini, N Tonna, M Meregalli, M Belicchi, S Erratico, P D'Ursi, F Bianco, M Legato, C Ruocco, C Sitzia, S Sangiorgi, C Villa, G D'Antona, L Milanesi, E Nisoli, P Mauri, Y Torrente
    EMBO Mol Med, 2019-12-02;0(0):e11019.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Insulin stimulates PI3K/AKT and cell adhesion to promote the survival of individualized human embryonic stem cells
    Authors: C Godoy-Pare, C Deng, W Liu, G Chen
    Stem Cells, 2019-05-17;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. The APMAP interactome reveals new modulators of APP processing and beta-amyloid production that are altered in Alzheimer's disease
    Authors: H Gerber, S Mosser, B Boury-Jamo, M Stumpe, A Piersigill, C Goepfert, J Dengjel, U Albrecht, F Magara, PC Fraering
    Acta Neuropathol Commun, 2019-01-31;7(1):13.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. IGF-II promotes neuroprotection and neuroplasticity recovery in a long-lasting model of oxidative damage induced by glucocorticoids
    Authors: E Martín-Mon, C Millon, F Boraldi, F Garcia-Gui, C Pedraza, E Lara, LJ Santin, J Pavia, M Garcia-Fer
    Redox Biol, 2017-05-26;13(0):69-81.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  5. mTORC1 regulates mannose-6-phosphate receptor transport and T-cell vulnerability to regulatory T cells by controlling kinesin KIF13A
    Authors: KA Ahmed, J Xiang
    Cell Discov, 2017-04-25;3(0):17011.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. A subset of bone marrow stromal cells regulate ATP-binding cassette gene expression via insulin-like growth factor-I in a leukemia cell line.
    Authors: Benabbou N, Mirshahi P, Bordu C, Faussat A, Tang R, Therwath A, Soria J, Marie J, Mirshahi M
    Int J Oncol, 2014-07-29;45(4):1372-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. A critical role for IGF-II in memory consolidation and enhancement.
    Authors: Chen DY, Stern SA, Garcia-Osta A, Saunier-Rebori B, Pollonini G, Bambah-Mukku D, Blitzer RD, Alberini CM
    Nature, 2011-01-27;469(7331):491-7.
    Species: Rat
    Sample Types: In Vivo
    Applications: Neutralization
  8. IGF2 actions on trophoblast in human placenta are regulated by the insulin-like growth factor 2 receptor, which can function as both a signaling and clearance receptor.
    Authors: Harris LK, Crocker IP, Baker PN, Aplin JD, Westwood M
    Biol. Reprod., 2010-10-27;84(3):440-6.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  9. CREG inhibits migration of human vascular smooth muscle cells by mediating IGF-II endocytosis.
    Authors: Han Y, Cui J, Tao J, Guo L, Guo P, Sun M, Kang J, Zhang X, Yan C, Li S
    Exp. Cell Res., 2009-09-19;315(19):3301-11.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Neutralization, Western Blot
  10. Urokinase induces survival or pro-apoptotic signals in human mesangial cells depending on the apoptotic stimulus.
    Authors: Tkachuk N, Kiyan J, Tkachuk S, Kiyan R, Shushakova N, Haller H, Dumler I
    Biochem. J., 2008-10-15;415(2):265-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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