Human IGF-II R/IGF2R Biotinylated Antibody Summary
Ser1510-Phe2108
Accession # P11717
Applications
Human IGF-II R/IGF2R Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of IGF-II R/IGF2R in Human Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Human IGF-II R/IGF2R Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2447, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGF-II R/IGF2R
The type 2 insulin-like growth factor receptor (also known as cation-independent mannose-6 phosphate receptor/CI-MPR) is a 300 kDa member of the P-type lectin family of molecules. P-type lectins generate functional eukaryotic lysosomes by binding and sorting lysosomal enzymes expressing phosphorylated mannose residues (M6P) (1‑3). IGF-II R is a type I transmembrane glycoprotein that contains a 2,264 amino acid (aa) extracellular region, a 23 aa transmembrane segment and a 124 aa cytoplasmic tail (4, 5). The extracellular region consists of 15 contiguous “binding” repeats of about 150 aa each. The odd-numbered repeats interact with “ligands” while the even-numbered repeats likely generate a nondisulfide homodimer in the membrane (1). Repeat #11 binds IGF-II, while repeats 3 and 9 bind mannose-6 phosphate; repeat #13 contains a fibronectin type II motif and assists in IGF-II binding (1, 2). In the extracellular region of IGF-II R expressed by R&D Systems (600 aa’s), human IGF-II R is 85% aa identical to both mouse and bovine IGF-II R. This expressed region includes binding repeats #11, 12, and 13. In addition to IGF-II, CI-MPR/IGF-II R binds non-M6P containing ligands such as retinoic acid, urokinase-type plasminogen-activator receptor and plasminogen, plus M6P‑containing molecules such as lysosomal enzymes, TGF-beta 1 precursor, proliferin, LIF, CD26, herpes simplex glycoprotein D and granzymes A and B (2, 6). IGF-II R regulates many diverse biological functions that range from intracellular trafficking to the internalization of extracellular factors and modulation of cellular responses. It delivers newly synthesized M6P‑tagged lysosomal enzymes from the trans-golgi network to endosomes, and facilitates the clearance of extracellular lysosomal and matrix degrading enzymes by internalization into clathrin-coated vesicles and delivery into endosomes. With respect to IGF-II biology, it would appear that IGF‑II R is principally a regulator of local IGF-II levels, targeting IGF-II for destruction in lysosomes (2). However, some evidence suggests the receptor will signal via G‑proteins, an effect that has yet to be conclusively shown (6).
- Ghosh, P. et al. (2003) Nat. Rev. Mol. Cell. Biol. 4:202.
- Dahms, N.M. and M.K. Hancock (2002) Biochim. Biophys. Acta. 1572:317.
- Zaina, S. and J. Nilsson (2003) Curr. Opin. Lipidol. 14:483.
- Morgan, D.O. et al. (1987) Nature 329:301.
- Oshima, A. et al. (1988) J. Biol. Chem. 263:2553.
- Hawkes, C. and S. Kar (2004) Brain Res. Rev. 44:117.
Product Datasheets
Citation for Human IGF-II R/IGF2R Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
CD44(+) fibroblasts increases breast cancer cell survival and drug resistance via IGF2BP3-CD44-IGF2 signalling
Authors: Y Liu, C Yu, Y Wu, X Sun, Q Su, C You, H Xin
J. Cell. Mol. Med., 2017-05-18;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human IGF-II R/IGF2R Biotinylated Antibody
There are currently no reviews for this product. Be the first to review Human IGF-II R/IGF2R Biotinylated Antibody and earn rewards!
Have you used Human IGF-II R/IGF2R Biotinylated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
