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Human IL-1 RI Antibody

Catalog #: AF269
8 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF269
AF269-SP
Detection of IL‑1 RI in HUVEC Human Cells by Flow Cytometry.
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Product Details
Citations (8)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-1 RI Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-1 RI in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-1 RI
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑1 RI
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced CXCL1/GRO alpha secretion in the HT‑29 human colon adenocarcinoma cell line.The Neutralization Dose (ND50) is typically < 5 µg/mL in the presence of 400 pg/mL Recombinant Human IL‑1 beta /IL‑1F2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL‑1 RI antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of IL‑1 RI in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were labeled with Human IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Neutralization CXCL1/GRO alpha Secretion Induced by IL‑1 beta /IL‑1F2 and Neutralization by Human IL‑1 RI Antibody. View Larger

CXCL1/GRO alpha Secretion Induced by IL‑1 beta /IL‑1F2 and Neutralization by Human IL‑1 RI Antibody. Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) induces CXCL1/GROa secretion in the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line),as measured by the Human CXCL1/GROa DuoSet (Catalog # DY275). CXCL1/GROa secretion elicited by Recombinant Human IL-1 beta /IL-1F2 is neutralized (green line) by increasing concentrations of Human IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269). The ND50 is typically < 5 µg/mL in the presence of 400 pg/mL Recombinant Human IL-1 beta /IL-1F2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 RI

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex.

An IL-1 receptor accessory protein that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.

References
  1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.
Long Name
Interleukin 1 Receptor I
Entrez Gene IDs
3554 (Human); 16177 (Mouse)
Alternate Names
CD121 antigen-like family member A; CD121a antigen; CD121a; IL-1 RI; IL1R; IL1R1; IL1RI; IL-1RI; IL1RT1; IL-1RT1; IL-1RT-1; Interleukin 1 Receptor 1; interleukin 1 receptor, type I; interleukin receptor 1; interleukin-1 receptor type 1; p80

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Citations for Human IL-1 RI Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Cancer genes disfavoring T�cell immunity identified via integrated systems approach
    Authors: RJ Kishton, SJ Patel, AE Decker, SK Vodnala, M Cam, TN Yamamoto, Y Patel, M Sukumar, Z Yu, M Ji, AN Henning, D Gurusamy, DC Palmer, RA Stefanescu, AT Girvin, W Lo, A Pasetto, P Malekzadeh, DC Deniger, KC Wood, NE Sanjana, NP Restifo
    Cell Reports, 2022-08-02;40(5):111153.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Expression and function of IL-1R8 (TIR8/SIGIRR), a regulatory member of the IL-1 receptor family in platelets
    Authors: Achille Anselmo
    Cardiovasc Res, 2016-06-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways.
    Authors: Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D
    J Immunol, 2015-08-14;195(6):2818-28.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma.
    Authors: Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R
    PLoS ONE, 2015-01-06;10(1):e0116772.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking, ICC
  5. IL-1beta promotes the differentiation of polyfunctional human CCR6+CXCR3+ Th1/17 cells that are specific for pathogenic and commensal microbes.
    Authors: Duhen T, Campbell D
    J Immunol, 2014-06-02;193(1):120-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta.
    Authors: Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F
    Nature, 2012-04-26;484(7395):514-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Chorionic gonadotropin down-regulates the expression of the decoy inhibitory interleukin 1 receptor type II in human endometrial epithelial cells.
    Authors: Herrmann-Lavoie C, Rao CV, Akoum A
    Endocrinology, 2007-08-16;148(11):5377-84.
    Species: Human
    Sample Types: Cell Culture Supernates, Cell Lysates
    Applications: ELISA Development, Western Blot
  8. Interleukin 1 in oviductal tissues of viviparous, oviparous, and ovuliparous species of amphibians.
    Authors: Jantra S, Bigliardi E, Brizzi R, Ietta F, Bechi N, Paulesu L
    Biol. Reprod., 2007-02-21;76(6):1009-15.
    Species: Amphibian
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot

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Human IL-1 RI Antibody
By Anonymous on 05/17/2019
Application: ELISA Sample Tested: Serum Species: Human
ELISA Human IL-1 RI Antibody AF269
Human IL-1 RI Antibody
By Abby Tyler on 11/09/2017
Application: ELISA Sample Tested: Cell culture supernatant,Serum and Plasma Species: Human

This polyclonal antibody (AF269) was used as both capture and detection for a sandwich assay for sIL-1R1. The immunoassay standard used was recombinant IL-1R1, 269-1R.

ELISA Human IL-1 RI Antibody AF269