Human IL-10 Antibody

Catalog #: MAB2171
12 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2171-SP
MAB2171-100
MAB2171-500

Detection of Human IL‑10 by Western Blot.

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All IL-10 Antibodies

Product Details

Citations (12)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-10 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse IL‑10.

Source

Monoclonal Mouse IgG_2B Clone # 25209

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human IL-10
Ser19-Asn178
Accession # P22301

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

5 µg/mL

See below

Neutralization

Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9-2 mouse mast cell line. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND₅₀) is typically 0.005-0.03 µg/mL in the presence of 5 ng/mL Recombinant Human IL‑10.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IL‑10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2171) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization

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Cell Proliferation Induced by IL‑10 and Neutralization by Human IL‑10 Antibody. Recombinant Human IL-10 (Catalog # 217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2171). The ND₅₀ is typically 0.005-0.03 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.

Long Name

Interleukin 10

Entrez Gene IDs

3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)

Alternate Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human IL-10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Human menstrual blood-derived stromal/stem cells modulate functional features of natural killer cells
Authors: MR Shokri, M Bozorgmehr, A Ghanavatin, R Falak, M Aleahmad, S Kazemnejad, F Shokri, AH Zarnani
Sci Rep, 2019-07-10;9(1):10007.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Oral fibroblasts modulate the macrophage response to bacterial challenge
Authors: R Tzach-Nahm, R Nashef, O Fleissig, A Palmon, L Shapira, A Wilensky, G Nussbaum
Sci Rep, 2017-09-14;7(1):11516.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis
Authors: S Nakachi, S Sumitomo, Y Tsuchida, H Tsuchiya, M Kono, R Kato, K Sakurai, N Hanata, Y Nagafuchi, S Tateishi, H Kanda, T Okamura, K Yamamoto, K Fujio
Arthritis Res. Ther., 2017-05-16;19(1):97.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
S100A8/A9 mRNA induction in an ex vivo model of endotoxin tolerance: roles of IL-10 and IFNgamma.
Authors: Fontaine M, Planel S, Peronnet E, Turrel-Davin F, Piriou V, Pachot A, Monneret G, Lepape A, Venet F
PLoS ONE, 2014-06-23;9(6):e100909.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
HIV-1 Tat protein induces PD-L1 (B7-H1) expression on dendritic cells through tumor necrosis factor alpha- and toll-like receptor 4-mediated mechanisms.
Authors: Planes R, BenMohamed L, Leghmari K, Delobel P, Izopet J, Bahraoui E
J Virol, 2014-04-02;88(12):6672-89.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
IFN-gamma-rich environment programs dendritic cells toward silencing of cytotoxic immune responses.
Authors: Svajger U, Obermajer N, Jeras M
J Leukoc Biol, 2013-08-07;95(1):33-46.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation.
Authors: Nicolaidou V, Wong MM, Redpath AN
PLoS ONE, 2012-07-03;7(7):e39871.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
DC-SIGN ligation greatly affects dendritic cell differentiation from monocytes compromising their normal function.
Authors: Svajger U, Obermajer N, Anderluh M, Kos J, Jeras M
J. Leukoc. Biol., 2011-03-29;89(6):893-905.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Analysis of adhesion molecules, target cells, and role of IL-2 in human FOXP3+ regulatory T cell suppressor function.
Authors: Tran DQ, Glass DD, Uzel G, Darnell DA, Spalding C, Holland SM, Shevach EM
J. Immunol., 2009-03-01;182(5):2929-38.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
Eur. J. Immunol., 2008-04-01;38(4):1012-23.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Complement receptor 3 blockade promotes IL-12-Mediated Clearance of Porphyromonas gingivalis and Negates Its Virulence In Vivo.
Authors: Hajishengallis G, Shakhatreh MA, Wang M, Liang S
J. Immunol., 2007-08-15;179(4):2359-67.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Effect of IL-21 on NK cells derived from different umbilical cord blood populations.
Authors: Perez S, Mahaira L, Sotiropoulou P, Gritzapis A, Iliopoulou E, Niarchos D, Cacoullos N, Kavalakis Y, Antsaklis A, Sotiriadou N, Baxevanis C, Papamichail M
Int Immunol, 2005-11-15;18(1):49-58.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization