Human IL-12/IL-23 p40 Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
IL‑17 Secretion Induced by IL‑23 and Neutralization by Human IL‑12/IL‑23 p40 Antibody. In the presence of Recombinant Mouse IL-2 (10 ng/mL, Catalog # 402-ML), Recombinant Human IL-23 (Catalog # 1290-IL) stimulates IL-17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL-17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL-17 secretion elicited by Recombinant Human IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-12/IL-23 p40 Monoclonal Antibody (Catalog # MAB1510). The ND50 is typically 0.02-0.1 µg/mL. This antibody also neutralizes Human IL-12 induced activity in human PBLs. The ND50 is approximately 0.05-0.25 µg/mL in the presence of 1 ng/mL Recombinant Human IL-12.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-12/IL-23 p40
Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 is produced by macrophages and B lymphocytes and has multiple effects on T-cells and NK cells, including stimulation of cytotoxic activity, proliferation, and promotion of Th1 development as well as IFN-gamma and TNF production. IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDA (p40) subunit and a 35 kDa (p35) subunit. The p40 and p35 subunits by themselves have no IL-12 activity, the p40 dimer has been shown to bind the IL-12 receptor and to be an IL-12 antagonist. Free p35 has not been detected in supernatant solutions of cultured cells expressing only p35 or both p35 and p40 mRNAs. In contrast, p40 is secreted in excess of IL-12 in cells expressing both p35 and p40 mRNAs. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and mouse IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.
Product Datasheets
Citations for Human IL-12/IL-23 p40 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
8
Citations: Showing 1 - 8
Filter your results:
Filter by:
-
Sarcoma IL-12 overexpression facilitates NK cell immunomodulation
Authors: MJ Rademacher, A Cruz, M Faber, RAA Oldham, D Wang, JA Medin, NJ Schloemer
Scientific Reports, 2021-04-15;11(1):8321.
Species: Human
Sample Types: Cell Culture Supernates, Whole Cells
Applications: ELISA Capture, Neutralization -
Characterization of the interaction between human decidua parietalis mesenchymal stem/stromal cells and natural killer cells
Authors: MH Abumaree, E Bahattab, A Alsadoun, A Al Dosaima, FM Abomaray, T Khatlani, B Kalionis, MF El-Muzaini, AO Alawad, AS AlAskar
Stem Cell Res Ther, 2018-04-12;9(1):102.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Phage-assisted continuous evolution of proteases with altered substrate specificity
Authors: MS Packer, HA Rees, DR Liu
Nat Commun, 2017-10-16;8(1):956.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells.
Authors: Sommer A, Fabri M
PLoS ONE, 2015-06-24;10(6):e0130395.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Tumor-activated TCRgammadelta(+) T cells from gastric cancer patients induce the antitumor immune response of TCRalphabeta(+) T cells via their antigen-presenting cell-like effects.
Authors: Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
J Immunol Res, 2014-03-31;2014(0):593562.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade.
Authors: Olson B, Jankowska-Gan E, Becker J, Vignali D, Burlingham W, McNeel D
J Immunol, 2012-11-14;189(12):5590-601.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
Authors: Rao DA, Tracey KJ, Pober JS
J. Immunol., 2007-11-15;179(10):6536-46.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
Authors: Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
J. Immunol., 2005-02-15;174(4):2376-86.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human IL-12/IL-23 p40 Antibody
Average Rating: 4.5 (Based on 2 Reviews)
Have you used Human IL-12/IL-23 p40 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
