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Human IL-12/IL-23 p40 ELISA Kit - Quantikine

Catalog #: DP400
28 Citations 2 Reviews Datasheet / COA / SDS
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PDP400
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Human IL-12/IL-23 p40 Homodimer ELISA Cell Culture Supernate Standard Curve
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Citations (28)
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Reviews (2)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IL-12/IL-23 p40 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)
Sensitivity
15 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human IL-12 p40
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IL-12 p40 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-12 p40 in cell culture supernates, serum, and plasma. It contains recombinant human IL-12 p40 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-12 p40. Results obtained using natural human IL-12 p40 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-12 p40.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 119 290 871 127 315 891
Standard Deviation 5.1 11.4 35.5 11.8 19.8 60.6
CV% 4.3 3.9 4.1 9.3 6.3 6.8

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 151 380 1138 160 399 1145
Standard Deviation 9.9 27.2 62.3 11 22.3 63
CV% 6.6 7.2 5.5 6.9 5.6 5.5

Recovery

The recovery of IL-12 p40 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 96 85-105
Citrate Plasma (n=5) 100 85-112
EDTA Plasma (n=5) 101 92-113
Heparin Plasma (n=5) 96 89-106
Serum (n=5) 98 86-112

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of IL-12 p40 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-12/IL-23 p40 Homodimer ELISA Linearity

Scientific Data

N/A Human IL-12/IL-23 p40 Homodimer ELISA Cell Culture Supernate Standard Curve View Larger

Human IL-12/IL-23 p40 Homodimer ELISA Cell Culture Supernate Standard Curve

N/A Human IL-12/IL-23 p40 Homodimer ELISA Serum/Plasma Standard Curve View Larger

Human IL-12/IL-23 p40 Homodimer ELISA Serum/Plasma Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-12/IL-23 p40

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

Long Name:
Interleukin 12/Interleukin 23 p40
Entrez Gene IDs:
3593 (Human); 16160 (Mouse); 64546 (Rat); 397076 (Porcine); 403976 (Canine); 102124858 (Cynomolgus Monkey); 493741 (Feline); 694747 (Rhesus Macaque)
Alternate Names:
CLMF p40; CLMF; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL12 p40; IL-12 p40; IL-12 subunit p40; IL12B; IL-12B; IL-12BNK cell stimulatory factor chain 2; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40); interleukin-12 beta chain; interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NKSF; NKSF2; NKSF2IL12, subunit p40
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well.
  7.   For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  8.   Aspirate and wash 4 times.
    9. 200 µL Substrate Solution
  9.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    10. 50 µL Stop Solution
  10. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human IL-12/IL-23 p40 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. Clinical and Immunological Factors Associated with Recommended Trough Levels of Adalimumab and Infliximab in Patients with Crohn's Disease
    Authors: B Orts, A Gutierrez, L Madero, L Sempere, R Frances, P Zapater
    Frontiers in Pharmacology, 2022-01-03;12(0):795272.
    Species: Human
    Sample Types: Serum
  2. Cognitive functional therapy (CFT) compared with core training exercise (CTE) in patients with failed back surgery syndrome (FBSS): A study protocol for a randomized controlled trial
    Authors: L Avila, ML Neves, AR Abreu, CR Fiuza, L Fukusawa, N Meziat-Fil, AR Soares San
    Journal of bodywork and movement therapies, 2020-09-14;26(0):428-434.
    Species: Human
    Sample Types: Serum
  3. Inhibition of IRF5 cellular activity with cell-penetrating peptides that target homodimerization
    Authors: J Banga, D Srinivasan, CC Sun, CD Thompson, F Milletti, KS Huang, S Hamilton, S Song, AF Hoffman, YG Qin, B Matta, M LaPan, Q Guo, G Lu, D Li, H Qian, DR Bolin, L Liang, C Wartchow, J Qiu, M Downing, S Narula, N Fotouhi, JA DeMartino, SL Tan, G Chen, BJ Barnes
    Sci Adv, 2020-05-15;6(20):eaay1057.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Characterization of the interaction between human decidua parietalis mesenchymal stem/stromal cells and natural killer cells
    Authors: MH Abumaree, E Bahattab, A Alsadoun, A Al Dosaima, FM Abomaray, T Khatlani, B Kalionis, MF El-Muzaini, AO Alawad, AS AlAskar
    Stem Cell Res Ther, 2018-04-12;9(1):102.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Regulated Polarization of Tumor-Associated Macrophages by miR-145 via Colorectal Cancer-Derived Extracellular Vesicles
    Authors: H Shinohara, Y Kuranaga, M Kumazaki, N Sugito, Y Yoshikawa, T Takai, K Taniguchi, Y Ito, Y Akao
    J. Immunol., 2017-07-10;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Myeloid-derived suppressor cells are increased and correlated with type 2 immune responses, malnutrition, inflammation, and poor prognosis in patients with breast cancer
    Authors: K Gonda, M Shibata, T Ohtake, Y Matsumoto, K Tachibana, N Abe, H Ohto, K Sakurai, S Takenoshit
    Oncol Lett, 2017-06-02;14(2):1766-1774.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Dysregulated serum IL-23 and SIRT1 activity in peripheral blood mononuclear cells of patients with rheumatoid arthritis.
    Authors: Wendling D, Abbas W, Godfrin-Valnet M, Kumar A, Guillot X, Khan K, Vidon C, Coquard L, Toussirot E, Prati C, Herbein G
    PLoS ONE, 2015-03-23;10(3):e0119981.
    Species: Human
    Sample Types: Serum
  8. Depletion of Foxp3+ regulatory T cells increases severity of mechanical allodynia and significantly alters systemic cytokine levels following peripheral nerve injury.
    Authors: Lees J, Duffy S, Perera C, Moalem-Taylor G
    Cytokine, 2014-11-20;71(2):207-14.
    Species: Human
    Sample Types: Serum
  9. Free IL-12p40 monomer is a polyfunctional adaptor for generating novel IL-12-like heterodimers extracellularly.
    Authors: Abdi K, Singh N, Spooner E, Kessler B, Radaev S, Lantz L, Xiao T, Matzinger P, Sun P, Ploegh H
    J Immunol, 2014-05-12;192(12):6028-36.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  10. Co-culture of human bronchial fibroblasts and CD4+ T cells increases Th17 cytokine signature.
    Authors: Loubaki L, Hadj-Salem I, Fakhfakh R, Jacques E, Plante S, Boisvert M, Aoudjit F, Chakir J
    PLoS ONE, 2013-12-05;8(12):e81983.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Direct and indirect impairment of human dendritic cell function by virulent Francisella tularensis Schu S4.
    Authors: Chase JC, Celli J, Bosio CM
    Infect. Immun., 2008-11-03;77(1):180-95.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Evidence for a pathogenetic role of interleukin-18 in cutaneous lupus erythematosus.
    Authors: Wang D, Drenker M, Eiz-Vesper B, Werfel T, Wittmann M
    Arthritis Rheum., 2008-10-01;58(10):3205-15.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. The 3'UTR 1188 A/C polymorphism in the interleukin-12p40 gene (IL-12B) is associated with lepromatous leprosy in the west of Mexico.
    Authors: Alvarado-Navarro A, Montoya-Buelna M, Munoz-Valle JF, Lopez-Roa RI, Guillen-Vargas C, Fafutis-Morris M
    Immunol. Lett., 2008-04-28;118(2):148-51.
    Species: Human
    Sample Types: Serum
  14. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
    Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
    Eur. J. Immunol., 2008-04-01;38(4):1012-23.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. First in human phase I trial of 852A, a novel systemic toll-like receptor 7 agonist, to activate innate immune responses in patients with advanced cancer.
    Authors: Dudek AZ, Yunis C, Harrison LI, Kumar S, Hawkinson R, Cooley S, Vasilakos JP, Gorski KS, Miller JS
    Clin. Cancer Res., 2007-12-01;13(23):7119-25.
    Species: Human
    Sample Types: Serum
  16. Impairment of human NK cell cytotoxic activity and cytokine release by cigarette smoke.
    Authors: Mian MF, Lauzon NM, Stampfli MR, Mossman KL, Ashkar AA
    J. Leukoc. Biol., 2007-11-30;83(3):774-84.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. 1, 25 Dihydroxyvitamin D3 modulated cytokine response in pulmonary tuberculosis.
    Authors: Vidyarani M, Selvaraj P, Jawahar MS, Narayanan PR
    Cytokine, 2007-10-29;40(2):128-34.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
    Authors: Lin YL, Liang YC, Chiang BL
    J. Leukoc. Biol., 2007-08-30;82(6):1473-80.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Oral resiquimod in chronic HCV infection: safety and efficacy in 2 placebo-controlled, double-blind phase IIa studies.
    Authors: Pockros PJ, Guyader D, Patton H, Tong MJ, Wright T, McHutchison JG, Meng TC
    J. Hepatol., 2007-05-04;47(2):174-82.
    Species: Human
    Sample Types: Serum
  20. Chemokines CXCL10 and CXCL11 in the cerebrospinal fluid of patients with tick-borne encephalitis.
    Authors: Lepej SZ, Misic-Majerus L, Jeren T, Rode OD, Remenar A, Sporec V, Vince A
    Acta Neurol. Scand., 2007-02-01;115(2):109-14.
    Species: Human
    Sample Types: Serum
  21. Induction of dendritic cell maturation by pertussis toxin and its B subunit differentially initiate Toll-like receptor 4-dependent signal transduction pathways.
    Authors: Wang ZY, Yang D, Chen Q, Leifer CA, Segal DM, Su SB, Caspi RR, Howard ZO, Oppenheim JJ
    Exp. Hematol., 2006-08-01;34(8):1115-24.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. IL-23 is increased in dendritic cells in multiple sclerosis and down-regulation of IL-23 by antisense oligos increases dendritic cell IL-10 production.
    Authors: Vaknin-Dembinsky A, Balashov K, Weiner HL
    J. Immunol., 2006-06-15;176(12):7768-74.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Bordetella pertussis inhibition of interleukin-12 (IL-12) p70 in human monocyte-derived dendritic cells blocks IL-12 p35 through adenylate cyclase toxin-dependent cyclic AMP induction.
    Authors: Spensieri F, Fedele G, Fazio C, Nasso M, Stefanelli P, Mastrantonio P, Ausiello CM
    Infect. Immun., 2006-05-01;74(5):2831-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Human complete Stat-1 deficiency is associated with defective type I and II IFN responses in vitro but immunity to some low virulence viruses in vivo.
    Authors: Chapgier A, Wynn RF, Jouanguy E, Filipe-Santos O, Zhang S, Feinberg J, Hawkins K, Casanova JL, Arkwright PD
    J. Immunol., 2006-04-15;176(8):5078-83.
    Species: Human
    Sample Types: Plasma
  25. Elevation of IL-12 p40 and its antibody in myasthenia gravis with thymoma.
    Authors: Yoshikawa H, Sato K, Edahiro S, Furukawa Y, Maruta T, Iwasa K, Watanabe H, Takaoka S, Suzuki Y, Takamori M, Yamada M
    J. Neuroimmunol., 2006-03-29;175(1):169-75.
    Species: Human
    Sample Types: Serum
  26. Experimental heatstroke in baboon: analysis of the systemic inflammatory response.
    Authors: Bouchama A, Ollivier V, Roberts G, Al Mohanna F, de Prost D, Eldali A, Saussereau E, El-Sayed R, Chollet-Martin S
    Shock, 2005-10-01;24(4):332-5.
    Species: Primate - Papio hamadryas (Hamadyras Baboon)
    Sample Types: Plasma
  27. Cytokines and adhesion molecules in multiple sclerosis patients treated with interferon-beta1b.
    Authors: Jensen J, Krakauer M, Sellebjerg F
    Cytokine, 2005-01-07;29(1):24-30.
    Species: Human
    Sample Types: Plasma
  28. HIV-1 protein Vpr suppresses IL-12 production from human monocytes by enhancing glucocorticoid action: potential implications of Vpr coactivator activity for the innate and cellular immunity deficits observed in HIV-1 infection.
    Authors: Mirani M, Elenkov I, Volpi S, Hiroi N, Chrousos GP, Kino T
    J. Immunol., 2002-12-01;169(11):6361-8.
    Species: Human
    Sample Types: Cell Culture Supernates

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Reviews for Human IL-12/IL-23 p40 Quantikine ELISA Kit

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Human IL-12/IL-23 p40 Quantikine ELISA Kit
By Maria Yanez on 05/10/2018
Sample Tested: Macrophage cytokine expression
Human IL-12/IL-23 p40 Quantikine ELISA Kit DP400
Human IL-12/IL-23 p40 Quantikine ELISA Kit
By Anonymous on 10/12/2017
Sample Tested: D3 mouse embryonic stem cell line