Human IL-13 Antibody Summary
Gly35-Asn146
Accession # P35225
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑13 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 5 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 10 µg/mL Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) for 3 days were stained with Mouse Anti-Human IL-13 Monoclonal Antibody (Catalog # MAB2131) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P). Quadrant markers were set based on control antibody staining (Catalog # MAB002).
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Cell Proliferation Induced by IL‑13 and Neutralization by Human IL‑13 Antibody. Recombinant Human IL-13 (Catalog # 213-ILB) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-13 (10 ng/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-Human IL-13 Monoclonal Antibody (Catalog # MAB2131). The ND50 is typically 1-4 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-13
IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature human IL-13 shares 57%, 59%, and 94% amino acid sequence identity with mouse, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is over-expressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).
- Wills-Karp, M. (2004) Immunol. Rev. 202:175.
- Nakajima H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
- McKenzie, A.N. et al. (1993) Proc. Natl. Acad. Sci. USA 90:3735.
- Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
- Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
- Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
- Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
- Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
- Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
- Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
- Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
- Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387.
- Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464.
- Tabata, Y. et al. (2007) J. Immunol. 177:7905.
- Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
- Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
- Andrews, A-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.
Product Datasheets
Citations for Human IL-13 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Restoring regulatory T-cell dysfunction in Alzheimer’s disease through ex vivo expansion
Authors: Alireza Faridar, Aaron D Thome, Weihua Zhao, Jason R Thonhoff, David R Beers, Belen Pascual et al.
Brain Communications
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Human CD8+ T-cells recognizing peptides from Mycobacterium tuberculosis (Mtb) presented by HLA-E have an unorthodox Th2-like, multifunctional, Mtb inhibitory phenotype and represent a novel human T-cell subset.
Authors: van Meijgaarden K, Haks M, Caccamo N, Dieli F, Ottenhoff T, Joosten S
PLoS Pathog, 2015-03-24;11(3):e1004671.
Species: Human
Sample Types: Whole Cells
Applications: Blocking -
In vivo inhibition of angiogenesis by interleukin-13 gene therapy in a rat model of rheumatoid arthritis.
Authors: Haas CS, Amin MA, Ruth JH, Allen BL, Ahmed S, Pakozdi A, Woods JM, Shahrara S, Koch AE
Arthritis Rheum., 2007-08-01;56(8):2535-48.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Neutralization
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