Human IL-17RA/IL-17R Antibody

Catalog #: MAB177
17 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB177-SP
MAB177-100
MAB177-500

Detection of IL-17 R in K562 Human Cell Line by Flow Cytometry.

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Product Details

Citations (17)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-17RA/IL-17R Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL‑17 R, rmIL‑17B R, or recombinant human IL‑17B R is observed. In Western blots, no cross-reactivity with rmIL-17 R is observed

Source

Monoclonal Mouse IgG₁ Clone # 133617

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL‑17 RA/IL‑17 R
Leu33-Trp320
Accession # Q96F46

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human IL‑17 RA/IL‑17 R Fc Chimera (Catalog # 177-IR)

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Knockout Validated

IL-17 RA/IL-17 R is specifically detected in K562 myelogenous leukemia parental cell line but is not detectable in IL-17 RA/IL-17 R knockout K562 cell line.

Neutralization

Measured by its ability to neutralize IL‑17/IL‑17A-induced CXCL1/GRO alpha secretion in the HT‑29 human colon adenocarcinoma cell line. The Neutralization Dose (ND₅₀) is typically 0.4-2 μg/mL in the presence of 40 ng/mL Recombinant Human IL‑17/IL‑17A.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IL-17 R in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 R Monoclonal Antibody (Catalog # MAB177, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Neutralization

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CXCL1/GRO alpha Secretion Induced by IL‑17/IL‑17A and Neutralization by Human IL‑17 RA/IL‑17 R Antibody. Recombinant Human IL-17/IL-17A induces CXCL1/GROa secretion in the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human CXCL1/GROa DuoSet (Catalog # DY275). CXCL1/GROa secretion elicited by Recombinant Human IL-17/IL-17A (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB177). The ND₅₀ is typically 0.4-2 µg/mL.

Knockout Validated

IL-17 RA/IL-17 R Antibody Specificity is Shown by Flow Cytometry in Knockout Cell Line.

View Larger

IL-17 RA/IL-17 R Specificity is Shown by Flow Cytometry in Knockout Cell Line. IL-17 RA/IL-17 R knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB177, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the IL-17 RA/IL-17 R knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RA/IL-17R

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL-17 R is a 120 kDa, 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. Within the ECD, human IL-17 R shares 72% aa sequence identity with mouse and rat IL‑17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.

References

Yao, Z. et al. (1997) Cytokine 9:794.
Schwander, R. et al. (2000) J. Exp. Med. 191:1233.

Long Name

Interleukin 17 Receptor

Entrez Gene IDs

23765 (Human); 16172 (Mouse); 312679 (Rat); 486759 (Canine); 101925679 (Cynomolgus Monkey)

Alternate Names

CD217 antigen; CD217; Cdw217; CDw217interleukin 17 receptor; hIL-17R; IL-17 R; IL-17 RA; IL-17 receptor A; IL17RA; IL-17RA; IL-17RAMGC10262; IL17Rinterleukin-17 receptor A; interleukin 17 receptor A

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human IL-17RA/IL-17R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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Evidence for anti-inflammatory effects and modulation of neurotransmitter metabolism by Salvia officinalis L
Authors: G Margetts, S Kleidonas, NS Zaibi, MS Zaibi, KD Edwards
BMC complementary medicine and therapies, 2022-05-12;22(1):131.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Impact of Adalimumab Treatment on Interleukin-17 and Interleukin-17 Receptor Expression in Skin and Synovium of Psoriatic Arthritis Patients with Mild Psoriasis
Authors: JW Bolt, AW van Kuijk, MBM Teunissen, D van der Co, S Aarrass, DM Gerlag, PP Tak, MG van de San, MC Lebre, LGM van Baarse
Biomedicines, 2022-01-29;10(2):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Interleukin-17A Triggers the Release of Platelet-Derived Factors Driving Vascular Endothelial Cells toward a Pro-Angiogenic State
Authors: A Gatsiou, K Sopova, A Tselepis, K Stellos
Cells, 2021-07-22;10(8):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Rapid and Rigorous IL-17A Production by a Distinct Subpopulation of Effector Memory T Lymphocytes Constitutes a Novel Mechanism of Toxic Shock Syndrome Immunopathology
Authors: PA Szabo, A Goswami, DM Mazzuca, K Kim, DB O'Gorman, DA Hess, ID Welch, HA Young, B Singh, JK McCormick, SM Haeryfar
J. Immunol, 2017-02-20;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Autophagy impacts on oxaliplatin-induced hepatocarcinoma apoptosis via the IL-17/IL-17R-JAK2/STAT3 signaling pathway
Authors: J Wu, J Guo, Q Cao, Y Wang, J Chen, Z Wang, Z Yuan
Oncol Lett, 2016-12-08;13(2):770-776.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Interleukin 17 inhibits progenitor cells in rheumatoid arthritis cartilage.
Authors: Schminke B, Trautmann S, Mai B, Miosge N, Blaschke S
Eur J Immunol, 2015-12-14;46(2):440-5.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Th17-related cytokines contribute to recall-like expansion/effector function of HMBPP-specific Vgamma2Vdelta2 T cells after Mycobacterium tuberculosis infection or vaccination.
Authors: Shen H, Wang Y, Chen C, Frencher J, Huang D, Yang E, Ryan-Payseur B, Chen Z
Eur J Immunol, 2015-02-01;45(2):442-51.
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Whole Cells
Applications: Neutralization
Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients.
Authors: Bellini A, Marini MA, Bianchetti L, Barczyk M, Schmidt M, Mattoli S
Mucosal Immunol, 2011-12-21;5(2):140-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
IL-17C regulates the innate immune function of epithelial cells in an autocrine manner.
Authors: Ramirez-Carrozzi V, Sambandam A, Luis E
Nat. Immunol., 2011-10-12;12(12):1159-66.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
TH17 cytokines induce human airway smooth muscle cell migration.
Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
J. Allergy Clin. Immunol., 2011-02-22;127(4):1046-53.e1-2.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

T-helper cell type 2 (Th2) memory T cell-potentiating cytokine IL-25 has the potential to promote angiogenesis in asthma.
Authors: Corrigan CJ, Wang W, Meng Q, Fang C, Wu H, Reay V, Lv Z, Fan Y, An Y, Wang YH, Liu YJ, Lee TH, Ying S
Proc. Natl. Acad. Sci. U.S.A., 2011-01-04;108(4):1579-84.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma.
Authors: Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D
Int. J. Cancer, 2009-09-01;125(5):1092-101.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

IL-17 induces monocyte migration in rheumatoid arthritis.
Authors: Shahrara S, Pickens SR, Dorfleutner A, Pope RM
J. Immunol., 2009-03-15;182(6):3884-91.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
Circulation, 2009-03-02;119(10):1424-32.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

IL-17RA and IL-17RC receptors are essential for IL-17A-induced ELR+ CXC chemokine expression in synoviocytes and are overexpressed in rheumatoid blood.
Authors: Zrioual S, Toh ML, Tournadre A, Zhou Y, Cazalis MA, Pachot A, Miossec V, Miossec P
J. Immunol., 2008-01-01;180(1):655-63.
Species: Human
Sample Types: RNA, Whole Tissue
Applications: IHC-P, Western Blot

CD molecules 2006--human cell differentiation molecules.
Authors: Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
J. Immunol. Methods, 2006-12-04;319(1):1-5.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
J. Immunol., 2005-11-01;175(9):6177-89.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry