Human IL-18 R beta /IL-1 R7 Antibody

Catalog #: MAB1181
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1181
MAB1181-SP

IFN-gamma secretion Induced by IL-18/IL1F4 and Neutralization by Human IL-18 R beta /IL-1 R7 Antibody.

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Citations (4)

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Human IL-18 R beta /IL-1 R7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑18 R beta /IL‑1 R7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse IL-18 R beta, recombinant human (rh) IL-18 R, rhIL-1 RI, rhIL-1 RII, rhIL-1 RAcP, and rhIL-1 Rrp2 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 132016

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL‑18 R beta /IL‑1 R7
Met1-Arg356
Accession # O95256

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human IL‑18 R beta /IL‑1 R7 Fc Chimera (Catalog # 118-AP)

Neutralization

Measured by its ability to neutralize IL-18/IL-1F4-induced IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line. Novick, D. et al. (1999) Immunity 10:127. The Neutralization Dose (ND₅₀) is typically 0.3-1.0 μg/mL in the presence of 40 ng/mL Recombinant Human IL-18/IL-1F4 and 20 ng/mL Recombinant Human TNF-alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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IFN-gamma secretion Induced by IL-18/IL1F4 and Neutralization by Human IL-18 R beta /IL-1 R7 Antibody. In the presence of Recombinant Human TNF-alpha (20 ng/mL, Catalog # 210-TA), Recombinant Human IL-18/ IL-1F4 stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (left-hand graph), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). Under these conditions, IFN-gamma secretion elicited by Recombinant Human IL-18/ IL-1F4 (40 ng/mL) is neutralized (right-hand graph) by increasing concentrations of Mouse Anti-Human IL-18 R beta /IL-1 R7 Monoclonal Antibody (Catalog # MAB1181). The ND₅₀ is typically 0.3-1.0 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-18 R beta/IL-1 R7

IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL-18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although
IL‑18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a 14 aa signal peptide, a 342 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon.

References

Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
Okamura, H. et al. (2000) in Cytokine Reference, Vol. 2:1605, Academic Press.

Long Name

Interleukin 18 Receptor beta

Entrez Gene IDs

8807 (Human); 16174 (Mouse)

Alternate Names

Accessory protein-like; AcPL; CD218 antigen-like family member B; CD218b antigen; CD218b; CDw218b; IL-1 R7; IL-18 R beta; IL-18 receptor accessory protein; IL-18 receptor beta; IL18R beta; IL-18RAcP; IL18RAP; IL18RB; IL-18Rb; IL-18Rbeta; IL-18R-beta; IL-1R accessory protein-like; IL1R7; IL-1R7; IL-1R-7; IL-1RAcPL; interleukin 18 receptor accessory protein; Interleukin-1 receptor 7; interleukin-18 receptor accessory protein; Interleukin-18 receptor accessory protein-like; Interleukin-18 receptor beta; MGC120589; MGC120590

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Citations for Human IL-18 R beta /IL-1 R7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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The IL18RAP region disease polymorphism decreases IL-18RAP/IL-18R1/IL-1R1 expression and signaling through innate receptor-initiated pathways.
Authors: Hedl M, Zheng S, Abraham C
J Immunol, 2014-05-19;192(12):5924-32.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis.
Authors: Takei S, Hoshino T, Matsunaga K, Sakazaki Y, Sawada M, Oda H, Takenaka S, Imaoka H, Kinoshita T, Honda S, Ida H, Fukuda TA, Aizawa H
Arthritis Res. Ther., 2011-03-24;13(2):R52.
Species: Human
Sample Types: Serum
Applications: Western Blot
CD molecules 2006--human cell differentiation molecules.
Authors: Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
J. Immunol. Methods, 2006-12-04;319(1):1-5.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
IL-18 receptor beta-induced changes in the presentation of IL-18 binding sites affect ligand binding and signal transduction.
Authors: Wu C, Sakorafas P, Miller R, McCarthy D, Scesney S, Dixon R, Ghayur T
J. Immunol., 2003-06-01;170(11):5571-7.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry