Human IL-19 Antibody

Catalog #: MAB1035 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1035
MAB1035-SP

Cell Proliferation Induced by IL‑19 and Neutralization by Human IL‑19 Antibody.

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All IL-19 Antibodies

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Human IL-19 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-19 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 152112

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IL-19
Leu25-Ala177
Accession # AAN40906

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

Human LPS-treated peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin

Neutralization

Measured by its ability to neutralize IL‑19-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta. The Neutralization Dose (ND₅₀) is typically 0.2-0.5 µg/mL in the presence of 3 ng/mL Recombinant Human IL‑19.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑19 and Neutralization by Human IL‑19 Antibody. Recombinant Human IL-19 (Catalog # 1035-IL) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-20 Ra and IL-20 R beta in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-19 (3 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-19 Monoclonal Antibody (Catalog # MAB1035). The ND₅₀ is typically 0.2-0.5 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-19

Human Interleukin 19 (IL-19) is a member of the IL-10 family of related cytokines. Its gene contains two alternate translation initiation sites, generating precursors of 215 amino acids (aa) and 177 aa, respectively. Both isoforms are processed to 17 kDa, 153 aa mature molecules. IL-19 contains seven helices and is secreted as a 35 kDa monomer. There are two potential N-linked glycosylation sites, and it is likely that the molecule is glycosylated. Mature human IL-19 shares 69% aa sequence identity with the mature mouse homologue. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells. IL-19 expression is limited to activated keratinocytes and monocytes. IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (IL-20 R alpha, also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R beta or IL-20 R2). This receptor complex is also shared by IL-20 and IL-24. Functionally, IL-19 induces IL-6 and TNF-alpha production by monocytes, and drives T-helper cell differentiation towards a Th2 response (1‑5).

References

Gallagher, G. et al. (2000) Genes Immun. 1:442.
Gallagher, G. et al. (2004) Int. Immunopharmacol. 4:615.
Laio, Y-C. et al. (2002) J. Immunol. 169:4288.
Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306.
Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.

Long Name

Interleukin 19

Entrez Gene IDs

29949 (Human); 329244 (Mouse)

Alternate Names

IL-10C; IL19; IL-19; interleukin 19; MDA1; melanoma differentiation associated protein-like protein; NG.1Melanoma differentiation-associated protein-like protein; ZMDA1interleukin-19