Human IL-1 beta /IL-1F2 Antibody Summary
aa 117-269
Accession # P01584
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB201) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-1 beta /IL-1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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IL‑1 beta /IL‑1F2 in Human PBMCs. IL‑1 beta /IL‑1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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IL‑1 beta /IL‑1F2 in Human PBMCs. IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Detection of Human IL‑1 beta /IL‑1F2 by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nm PMA and 10 ug/ml LPS for 24 hrs and 3 hrs, respectively, loaded at 0.2 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 38 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB201). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Human IL‑1 beta /IL‑1F2 Antibody. Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB201). The ND50 is typically 0.001-0.003 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.
Product Datasheets
Citations for Human IL-1 beta /IL-1F2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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A therapeutic strategy of parthenolide in improving imiquimod-induced psoriasis-like skin inflammation targeting IL-36/NETs through skin transdermal therapeutic system
Authors: Zhan, ZY;Zhang, ZH;Sun, RH;Wu, YL;Nan, JX;Lian, LH;
International immunopharmacology
Species: Human hepegivirus
Sample Types: Cell Lysates
Applications: Western Blot -
Mesothelial cells with mesenchymal features enhance peritoneal dissemination by forming a protumorigenic microenvironment
Authors: Yonemura, A;Semba, T;Zhang, J;Fan, Y;Yasuda-Yoshihara, N;Wang, H;Uchihara, T;Yasuda, T;Nishimura, A;Fu, L;Hu, X;Wei, F;Kitamura, F;Akiyama, T;Yamashita, K;Eto, K;Iwagami, S;Iwatsuki, M;Miyamoto, Y;Matsusaki, K;Yamasaki, J;Nagano, O;Saya, H;Song, S;Tan, P;Baba, H;Ajani, JA;Ishimoto, T;
Cell reports
Species: Murine polyomavirus strain A3
Sample Types: Whole Cells
Applications: Mass Cytometry -
Yersinia Type III-Secreted Effectors Subvert Caspase-4-dependent Inflammasome Activation in Human Cells
Authors: J Zhang, IE Brodsky, S Shin
bioRxiv : the preprint server for biology, 2023-01-25;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Macrophages activated by hepatitis B virus have distinct metabolic profiles and suppress the virus via IL-1beta to downregulate PPARalpha and FOXO3
Authors: Y Li, Y Zhu, S Feng, Y Ishida, TP Chiu, T Saito, S Wang, DK Ann, JJ Ou
Cell Reports, 2022-01-25;38(4):110284.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Gasdermin D mediates the maturation and release of IL-1&alpha downstream of inflammasomes
Authors: K Tsuchiya, S Hosojima, H Hara, H Kushiyama, MR Mahib, T Kinoshita, T Suda
Cell Reports, 2021-03-23;34(12):108887.
Species: Mouse
Sample Types: Peritoneal Lavage Fluid
Applications: Western Blot -
Burkholderia pseudomallei triggers canonical inflammasome activation in a human primary macrophage-based infection model
Authors: S Lichtenegg, J Stiehler, S Saiger, A Zauner, B Kleinhappl, C Bernecker, P Schlenke, GE Wagner, K Krause, M Gastager, I Steinmetz
PLoS Negl Trop Dis, 2020-11-02;14(11):e0008840.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
NLRP3 inflammasome function and pyroptotic cell death in human placental Hofbauer cells
Authors: VM Abrahams, Z Tang, G Mor, S Guller
J Reprod Immunol, 2020-10-03;142(0):103214.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Caspase-1 has a critical role in blood-brain barrier injury and its inhibition contributes to multifaceted repair
Authors: H Israelov, O Ravid, D Atrakchi, D Rand, S Elhaik, Y Bresler, R Twitto-Gre, L Omesi, S Liraz-Zalt, F Gosselet, M Schnaider, I Cooper
J Neuroinflammation, 2020-09-09;17(1):267.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Autoimmune inner ear disease patient-associated 28-kDa proinflammatory IL-1beta fragment results from caspase-7-mediated cleavage in vitro
Authors: S Pathak, A Vambutas
JCI Insight, 2020-02-13;5(3):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Microenvironmental IL1beta promotes breast cancer metastatic colonisation in the bone via activation of Wnt signalling
Authors: R Eyre, DG Alférez, A Santiago-G, K Spence, JC McConnell, C Hart, BM Simões, D Lefley, C Tulotta, J Storer, A Gurney, N Clarke, M Brown, SJ Howell, AH Sims, G Farnie, PD Ottewell, RB Clarke
Nat Commun, 2019-11-01;10(1):5016.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Developmental induction of human T-cell responses against Candida albicans and Aspergillus fumigatus
Authors: K Vogel, M Pierau, A Arra, K Lampe, D Schlueter, C Arens, MC Brunner-We
Sci Rep, 2018-11-15;8(1):16904.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Extracellular Neutrophil Proteases Are Efficient Regulators of IL-1, IL-33, and IL-36 Cytokine Activity but Poor Effectors of Microbial Killing
Authors: DM Clancy, GP Sullivan, HBT Moran, CM Henry, EP Reeves, NG McElvaney, EC Lavelle, SJ Martin
Cell Rep, 2018-03-13;22(11):2937-2950.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The Vasoreparative Function of Myeloid Angiogenic Cells Is Impaired in Diabetes Through the Induction of IL1?
Authors: SEJ Chambers, CL O'Neill, J Guduric-Fu, KJ McLoughlin, A Liew, AM Egan, T O'Brien, AW Stitt, RJ Medina
Stem Cells, 2018-03-09;36(6):834-843.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Neutralization, Western Blot -
Galectin-3 is an amplifier of the interleukin-1?-mediated inflammatory response in corneal keratinocytes
Authors: Y Uchino, AM Woodward, J Mauris, K Peterson, P Verma, UJ Nilsson, J Rajaiya, P Argüeso
Immunology, 2018-02-15;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Adipose progenitor cell secretion of GM-CSF and MMP9 promotes a stromal and immunological microenvironment that supports breast cancer progression
Authors: F Reggiani, V Labanca, P Mancuso, C Rabascio, G Talarico, S Orecchioni, A Manconi, F Bertolini
Cancer Res., 2017-07-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Opposing effects of Nrf2 and Nrf2-activating compounds on the NLRP3 inflammasome independent of Nrf2-mediated gene expression
Authors: M Garstkiewi, GE Strittmatt, S Grossi, J Sand, G Fenini, S Werner, LE French, HD Beer
Eur. J. Immunol, 2017-04-10;0(0):.
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Human Scavenger Receptor A1-Mediated Inflammatory Response to Silica Particle Exposure Is Size Specific
Authors: N Nishijima, T Hirai, K Misato, M Aoyama, E Kuroda, KJ Ishii, K Higashisak, Y Yoshioka, Y Tsutsumi
Front Immunol, 2017-04-03;8(0):379.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Processing and secretion of guanylate binding protein-1 depend on inflammatory caspase activity
Authors: E Naschberge, W Gei beta dörfer, C Bogdan, P Tripal, E Kremmer, M Stürzl, N Britzen-La
J. Cell. Mol. Med, 2017-03-08;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Differential IL-1? secretion by monocyte subsets is regulated by Hsp27 through modulating mRNA stability
Sci Rep, 2016-12-15;6(0):39035.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Downregulation of estrogen receptor and modulation of growth of breast cancer cell lines mediated by paracrine stromal cell signals
Authors: R A Steinman
Breast Cancer Res. Treat., 2016-11-16;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses
Authors: Wan-Chien Cheng
Eur J Immunol, 2016-08-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
NLRP3 inflammasome: a novel link between lipoproteins and atherosclerosis
Arch Med Sci, 2016-07-20;12(5):950-958.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Development of a Novel Model for the Assessment of Dead-Space Management in Soft Tissue.
Authors: Oliver R, Lovric V, Yu Y, Christou C, Aiken S, Cooper J, Walsh W
PLoS ONE, 2015-08-25;10(8):e0136514.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Neutrophil elastase promotes interleukin-1beta secretion from human coronary endothelium.
Authors: Alfaidi M, Wilson H, Daigneault M, Burnett A, Ridger V, Chamberlain J, Francis S
J Biol Chem, 2015-08-12;290(40):24067-78.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Herpesvirus Genome Recognition Induced Acetylation of Nuclear IFI16 Is Essential for Its Cytoplasmic Translocation, Inflammasome and IFN-beta Responses.
Authors: Ansari M, Dutta S, Veettil M, Dutta D, Iqbal J, Kumar B, Roy A, Chikoti L, Singh V, Chandran B
PLoS Pathog, 2015-07-02;11(7):e1005019.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Monocyte-derived dendritic cells from Crohn's disease patients exhibit decreased ability to activate T helper type 17 responses in memory cells.
Authors: Nieminen J, Sipponen T, Farkkila M, Vaarala O
Clin Exp Immunol, 2014-07-01;177(1):190-202.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation.
Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P
PLoS Biol, 2012-09-25;10(9):e1001395.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation.
Authors: Nicolaidou V, Wong MM, Redpath AN
PLoS ONE, 2012-07-03;7(7):e39871.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta.
Authors: Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F
Nature, 2012-04-26;484(7395):514-8.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
Authors: Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M
Eur. J. Immunol., 2009-05-01;39(5):1221-30.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Flightless-I regulates proinflammatory caspases by selectively modulating intracellular localization and caspase activity.
Authors: Li J, Yin HL, Yuan J
J. Cell Biol., 2008-04-14;181(2):321-33.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells.
Authors: Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F
Nat. Immunol., 2007-08-05;8(9):942-9.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
Dig. Dis. Sci., 2007-03-30;52(10):2775-83.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Neutrophil-derived elastase induces TGF-beta1 secretion in human airway smooth muscle via NF-kappaB pathway.
Authors: Lee KY, Ho SC, Lin HC, Lin SM, Liu CY, Huang CD, Wang CH, Chung KF, Kuo HP
Am. J. Respir. Cell Mol. Biol., 2006-05-11;35(4):407-14.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Intravenous human interleukin-1alpha impairs memory processing in mice: dependence on blood-brain barrier transport into posterior division of the septum.
Authors: Banks WA, Farr SA, La Scola ME
J. Pharmacol. Exp. Ther., 2001-11-01;299(2):536-41.
Species: Human
Sample Types: Recombinant Protein
Applications: Neutralization -
Amyloid beta and amylin fibrils induce increases in proinflammatory cytokine and chemokine production by THP-1 cells and murine microglia.
Authors: Yates SL, Burgess LH, Kocsis-Angle J, Antal JM, Dority MD, Embury PB, Piotrkowski AM, Brunden KR
J. Neurochem., 2000-03-01;74(3):1017-25.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
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We used this IL1B (5ng/mL) neutralizing antibody as part of an antibody cocktail containing several other antibodies to neutralize IL1B present in the Conditioned Medium (CM) from BT549 Cells treated with Angiotensin II. The effect of cytokines in the CM (+/-Neutralizing ab Cocktail) on Endothelial (HUVEC) Cell migration was measured using Incucyte Zoom Chemotaxis Assay.
