Human IL-1 beta /IL-1F2 Antibody Summary
Ala117-Ser269
Accession # NP_000567
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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IL-1 beta /IL-1F2 in Human Tonsil Using Dual RNAscope®ISH and IHC. IL-1 beta /IL-1F2 mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope®Probe (Catalog # 310361) and stained using ACD RNAscope®2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Goat Anti-Human IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) at 1 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).
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Detection of Human IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-1 beta /IL-1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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IL‑1 beta /IL‑1F2 in Human PBMCs. IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Goat Anti-Human IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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IL‑1 beta /IL‑1F2 in Human Tonsil. IL-1 beta /IL-1F2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of Human IL‑1 beta /IL‑1F2 by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nm PMA and 10 ug/ml LPS for 24 hrs and 3 hrs, respectively, and loaded at 0.2 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 39 kDa (as indicated) using 1 µg/mL of Goat Anti-Human IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Human IL‑1 beta /IL‑1F2 Antibody. Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA). The ND50 is typically 6-42 ng/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.
Product Datasheets
Citations for Human IL-1 beta /IL-1F2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Activation and functional modification of mucosal-associated invariant T cells in patients with intracranial infection following craniotomy
Authors: Pu, X;Bu, W;Qin, Y;Wang, C;Xu, L;Fang, M;Ji, Q;Wang, H;Shao, M;
International immunopharmacology
Species: Human hepegivirus
Sample Types: Whole Cells
Applications: Neutralization -
Interleukin-1?/Interleukin (IL)-1-Receptor-Antagonist (IL1-RA) Axis in Invasive Bladder Cancer-An Exploratory Analysis of Clinical and Tumor Biological Significance
Authors: Vukovic, M;Chamlati, JM;Hennenlotter, J;Todenhöfer, T;Lütfrenk, T;Jersinovic, S;Tsaur, I;Stenzl, A;Rausch, S;
International journal of molecular sciences
Species: Human hepegivirus
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
HIF1alpha-BNIP3-mediated mitophagy protects against renal fibrosis by decreasing ROS and inhibiting activation of the NLRP3 inflammasome
Authors: J Li, Q Lin, X Shao, S Li, X Zhu, J Wu, S Mou, L Gu, Q Wang, M Zhang, K Zhang, J Lu, Z Ni
Cell Death & Disease, 2023-03-17;14(3):200.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs
Authors: SI Gringhuis, TM Kaptein, EBM Remmerswaa, A Drewniak, BA Wevers, B Theelen, GRAM D'Haens, T Boekhout, TBH Geijtenbee
Nature Immunology, 2022-12-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
NLRP3 inflammasome triggers interleukin-37 release from human monocytes
Authors: A Gritsenko, R Díaz-Pino, G Lopez-Cast
European Journal of Immunology, 2022-04-28;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
IBD-associated G protein-coupled receptor 65 variant compromises signalling and impairs key functions involved in inflammation
Authors: V Mercier, G Boucher, D Devost, K Bourque, A Alikashani, C Beauchamp, A Bitton, S Foisy, P Goyette, G Charron, TE Hébert, JD Rioux
Cellular Signalling, 2022-02-24;93(0):110294.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
M24B aminopeptidase inhibitors selectively activate the CARD8 inflammasome
Authors: SD Rao, Q Chen, Q Wang, EL Orth-He, M Saoi, AR Griswold, A Bhattachar, DP Ball, HC Huang, AJ Chui, DJ Covelli, S You, JR Cross, DA Bachovchin
Nature Chemical Biology, 2022-02-14;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Single cell transcriptomic landscape of diabetic foot ulcers
Authors: G Theocharid, BE Thomas, D Sarkar, HL Mumme, WJR Pilcher, B Dwivedi, T Sandoval-S, RF Sîrbulescu, A Kafanas, I Mezghani, P Wang, A Lobao, IS Vlachos, B Dash, HC Hsia, V Horsley, SS Bhasin, A Veves, M Bhasin
Nature Communications, 2022-01-10;13(1):181.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Heme stimulates platelet mitochondrial oxidant production to induce targeted granule secretion
Authors: GK Annarapu, D Nolfi-Done, M Reynolds, Y Wang, L Kohut, B Zuckerbrau, S Shiva
Redox Biology, 2021-12-05;48(0):102205.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Dot Blot -
Endolysosomal ion channel MCOLN2 (Mucolipin-2) promotes prostate cancer progression via IL-1beta/NF-kappaB pathway
Authors: H Yu, M Xie, Z Meng, CY Lo, FL Chan, L Jiang, X Meng, X Yao
British Journal of Cancer, 2021-09-21;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
The Blockade of Tumoral IL1&beta-Mediated Signaling in Normal Colonic Fibroblasts Sensitizes Tumor Cells to Chemotherapy and Prevents Inflammatory CAF Activation
Authors: NG Díaz-Marot, G Garcia-Vic, G Polcaro, M Bañuls, N Albert, A Villanueva, DG Molleví
International Journal of Molecular Sciences, 2021-05-07;22(9):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Gasdermin E permits interleukin-1 beta release in distinct sublytic and pyroptotic phases
Authors: B Zhou, DW Abbott
Cell Reports, 2021-04-13;35(2):108998.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Glycyrrhizin prevents SARS-CoV-2 S1 and Orf3a induced high mobility group box 1 (HMGB1) release and inhibits viral replication
Authors: P Gowda, S Patrick, SD Joshi, RK Kumawat, E Sen
Cytokine, 2021-03-12;142(0):155496.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Structural basis for distinct inflammasome complex assembly by human NLRP1 and CARD8
Authors: Q Gong, K Robinson, C Xu, PT Huynh, KHC Chong, EYJ Tan, J Zhang, ZZ Boo, DET Teo, K Lay, Y Zhang, JSY Lim, WI Goh, G Wright, FL Zhong, B Reversade, B Wu
Nature Communications, 2021-01-08;12(1):188.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Inflammasome Sensor NLRP1 Confers Acquired Drug Resistance to Temozolomide in Human Melanoma
Authors: Z Zhai, JM Samson, T Yamauchi, PK Vaddi, Y Matsumoto, CA Dinarello, D Ravindran, M Fujita
Cancers (Basel), 2020-09-04;12(9):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Platelets Fuel the Inflammasome Activation of Innate Immune Cells
Authors: V Rolfes, LS Ribeiro, I Hawwari, L Böttcher, N Rosero, S Maasewerd, MLS Santos, T Próchnicki, CMS Silva, CWS Wanderley, M Rothe, SV Schmidt, HJ Stunden, D Bertheloot, MN Rivas, CJ Fontes, LH Carvalho, FQ Cunha, E Latz, M Arditi, BS Franklin
Cell Rep, 2020-05-12;31(6):107615.
Species: Human
Sample Types:
Applications: Blocking/Neutralizing -
Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin in?ammation
Authors: Z Wang, H Zhou, H Zheng, X Zhou, G Shen, X Teng, X Liu, J Zhang, X Wei, Z Hu, F Zeng, Y Hu, J Hu, X Wang, S Chen, J Cheng, C Zhang, Y Gui, S Zou, Y Hao, Q Zhao, W Wu, Y Zhou, K Cui, N Huang, Y Wei, W Li, J Li
Autophagy, 2020-02-16;0(0):1-24.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Interleukin 1&beta/1RA axis in colorectal cancer regulates tumor invasion, proliferation and apoptosis via autophagy
Authors: Y Chen, Z Yang, B Deng, D Wu, Y Quan, Z Min
Oncol. Rep., 2020-01-22;43(3):908-918.
Species: Human
Sample Types: Cell Culture Lysates, Whole Tissue
Applications: IHC, Western Blot -
Periodontal inflammation recruits distant metastatic breast cancer cells by increasing myeloid-derived suppressor cells
Authors: R Cheng, S Billet, C Liu, S Haldar, D Choudhury, M Tripathi, M Hav, A Merchant, T Hu, H Huang, H Zhou, NA Bhowmick
Oncogene, 2019-11-04;0(0):.
Species: Human, Mouse
Sample Types: Cell Lysate, Whole Tissue
Applications: IHC, Western Blot -
PINK1-parkin pathway of mitophagy protects against contrast-induced acute kidney injury via decreasing mitochondrial ROS and NLRP3 inflammasome activation
Authors: Q Lin, S Li, N Jiang, X Shao, M Zhang, H Jin, Z Zhang, J Shen, Y Zhou, W Zhou, L Gu, R Lu, Z Ni
Redox Biol, 2019-06-11;26(0):101254.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Characterization of the non-glandular gastric region microbiota in Helicobacter suis-infected versus non-infected pigs identifies a potential role for Fusobacterium gastrosuis in gastric ulceration
Authors: C De Witte, K Demeyere, S De Bruycke, B Taminiau, G Daube, R Ducatelle, E Meyer, F Haesebrouc
Vet. Res., 2019-05-24;50(1):39.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Enteropathogenic Escherichia coli Stimulates Effector-Driven Rapid Caspase-4 Activation in Human Macrophages
Authors: PJ Goddard, J Sanchez-Ga, SL Slater, M Kalyan, D Ruano-Gall, O Marchès, LÁ Fernández, G Frankel, AR Shenoy
Cell Rep, 2019-04-23;27(4):1008-1017.e6.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Human Cytomegalovirus Immediate Early 86-kDa Protein Blocks Transcription and Induces Degradation of the Immature Interleukin-1? Protein during Virion-Mediated Activation of the AIM2 Inflammasome
Authors: S Botto, J Abraham, N Mizuno, K Pryke, B Gall, I Landais, DN Streblow, KJ Fruh, VR DeFilippis
MBio, 2019-02-12;10(1):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
ROR? controls inflammatory state of human macrophages
Authors: N Nejati Moh, E Bjørkøy Ta, L Ryan, T Espevik, V Boyartchuk
PLoS ONE, 2018-11-28;13(11):e0207374.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The fungal peptide toxin Candidalysin activates the NLRP3 inflammasome and causes cytolysis in mononuclear phagocytes
Authors: L Kasper, A König, PA Koenig, MS Gresnigt, J Westman, RA Drummond, MS Lionakis, O Gro beta, J Ruland, JR Naglik, B Hube
Nat Commun, 2018-10-15;9(1):4260.
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ACN9 Regulates the Inflammatory Responses in Human Bronchial Epithelial Cells
Authors: JH Jeong, J Kim, J Kim, HR Heo, JS Jeong, YJ Ryu, Y Hong, SS Han, SH Hong, SJ Lee, WJ Kim
Tuberc Respir Dis (Seoul), 2017-07-03;80(3):247-254.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
An adaptive signaling network in melanoma inflammatory niches confers tolerance to MAPK signaling inhibition
Authors: HL Young, EJ Rowling, M Bugatti, E Giurisato, N Luheshi, I Arozarena, JC Acosta, J Kamarashev, DT Frederick, ZA Cooper, A Reuben, J Gil, KT Flaherty, JA Wargo, W Vermi, MP Smith, C Wellbrock, A Hurlstone
J. Exp. Med., 2017-04-27;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
The Regulation of Uterine Proinflammatory Gene Expression during Pregnancy in the Live-Bearing Lizard, Pseudemoia entrecasteauxii
Authors: K Hendrawan, CM Whittingto, MC Brandley, K Belov, MB Thompson
J. Exp. Zool. B Mol. Dev. Evol, 2017-03-10;0(0):.
Species: Lizard
Sample Types: Tissue Homogenates
Applications: Western Blot -
Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis
Authors: L Qiang, A Sample, CR Shea, K Soltani, KF Macleod, YY He
Autophagy, 2017-01-01;0(0):0.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
17-oxo-DHA displays additive anti-inflammatory effects with fluticasone propionate and inhibits the NLRP3 inflammasome
Sci Rep, 2016-11-24;6(0):37625.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Efficacy and Pharmacology of the NLRP3 Inflammasome Inhibitor CP-456,773 (CRID3) in Murine Models of Dermal and Pulmonary Inflammation
J Immunol, 2016-08-12;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS
Authors: Yong Hwan Park
Nat Immunol, 2016-06-06;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Acid-dependent Interleukin-1 (IL-1) Cleavage Limits Available Pro-IL-1beta for Caspase-1 Cleavage.
Authors: Edye M, Brough D, Allan S
J Biol Chem, 2015-08-31;290(42):25374-81.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot -
Cutting Edge: Inflammasome Activation in Primary Human Macrophages Is Dependent on Flagellin.
Authors: Kortmann J, Brubaker S, Monack D
J Immunol, 2015-06-24;195(3):815-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Prostaglandin E2 Inhibits NLRP3 Inflammasome Activation through EP4 Receptor and Intracellular Cyclic AMP in Human Macrophages.
Authors: Sokolowska M, Chen L, Liu Y, Martinez-Anton A, Qi H, Logun C, Alsaaty S, Park Y, Kastner D, Chae J, Shelhamer J
J Immunol, 2015-04-27;194(11):5472-87.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases.
Authors: Coll R, Robertson A, Chae J, Higgins S, Munoz-Planillo R, Inserra M, Vetter I, Dungan L, Monks B, Stutz A, Croker D, Butler M, Haneklaus M, Sutton C, Nunez G, Latz E, Kastner D, Mills K, Masters S, Schroder K, Cooper M, O'Neill L
Nat Med, 2015-02-16;21(3):248-55.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
IL-34- and M-CSF-induced macrophages switch memory T cells into Th17 cells via membrane IL-1alpha.
Authors: Foucher E, Blanchard S, Preisser L, Descamps P, Ifrah N, Delneste Y, Jeannin P
Eur J Immunol, 2015-01-19;45(4):1092-102.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Methylsulfonylmethane inhibits NLRP3 inflammasome activation.
Authors: Ahn H, Kim J, Lee M, Kim Y, Cho Y, Lee G
Cytokine, 2014-11-21;71(2):223-31.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Complement C5a potentiates uric acid crystal-induced IL-1beta production.
Authors: An L, Mehta P, Xu L, Turman S, Reimer T, Naiman B, Connor J, Sanjuan M, Kolbeck R, Fung M
Eur J Immunol, 2014-10-28;44(12):3669-79.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Early mucosal sensing of SIV infection by paneth cells induces IL-1beta production and initiates gut epithelial disruption.
Authors: Hirao L, Grishina I, Bourry O, Hu W, Somrit M, Sankaran-Walters S, Gaulke C, Fenton A, Li J, Crawford R, Chuang F, Tarara R, Marco M, Baumler A, Cheng H, Dandekar S
PLoS Pathog, 2014-08-28;10(8):e1004311.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Tissue
Applications: IHC -
Tumor-activated TCRgammadelta(+) T cells from gastric cancer patients induce the antitumor immune response of TCRalphabeta(+) T cells via their antigen-presenting cell-like effects.
Authors: Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
J Immunol Res, 2014-03-31;2014(0):593562.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages.
Authors: L'homme L, Esser N, Riva L, Scheen A, Paquot N, Piette J, Legrand-Poels S
J Lipid Res, 2013-09-03;54(11):2998-3008.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP.
Authors: Lee G, Subramanian N, Kim A, Aksentijevich I, Goldbach-Mansky R, Sacks D, Germain R, Kastner D, Chae J
Nature, 2012-11-11;492(7427):123-7.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Surface molecule CD229 as a novel target for the diagnosis and treatment of multiple myeloma.
Authors: Atanackovic D, Panse J, Hildebrandt Y, Jadczak A, Kobold S, Cao Y, Templin J, Meyer S, Reinhard H, Bartels K, Lajmi N, Zander AR, Marx AH, Luetkens T, Bokemeyer C, Kroger N
Haematologica, 2011-05-23;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes.
Authors: Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P
J. Immunol., 2011-03-02;186(7):4175-82.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
ADAMTS-1 metalloproteinase promotes tumor development through the induction of a stromal reaction in vivo.
Authors: Rocks N, Paulissen G, Quesada-Calvo F, Munaut C, Gonzalez ML, Gueders M, Hacha J, Gilles C, Foidart JM, Noel A, Cataldo DD
Cancer Res., 2008-11-15;68(22):9541-50.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Increased expression of interleukin-1beta and its novel splice variant in canine hearts with volume overload.
Authors: Kiczak L, Paslawska U, Bania J, Ugorski M, Sambor I, Kochman A, Blach J, Chelmonska-Soyta A
Cytokine, 2008-11-12;44(3):352-60.
Species: Canine
Sample Types: Whole Tissue
Applications: IHC-P -
Interleukin 1 in oviductal tissues of viviparous, oviparous, and ovuliparous species of amphibians.
Authors: Jantra S, Bigliardi E, Brizzi R, Ietta F, Bechi N, Paulesu L
Biol. Reprod., 2007-02-21;76(6):1009-15.
Species: Amphibian
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot -
Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
Authors: Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE
Photochem. Photobiol., 2004-09-01;80(2):231-5.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
Authors: Sengupta J, Dhawan L, Ghosh D
Cytokine, 2003-12-21;24(6):277-85.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Tissue
Applications: IHC-P
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These Abs recognize both pro-IL1b (p31) and active, cleaved form (p17) in cell lysates and conditioned media of activated human monocyte derived macrophages and THP-1 derived macrophages. To see p17 IL-1b I used 10% tricine gel due to small size of detected protein.
I use antibody at dilution 1:500
