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Human IL-20 Antibody

Catalog #: AF1102
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1102
AF1102-SP
Cell Proliferation Induced by IL‑20 and Neutralization by Human IL‑20 Antibody.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-20 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑20 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) IL‑19 is observed, approximately 15% cross-reactivity with recombinant mouse IL‑20 is observed, and less than 1% cross-reactivity with rhIL‑10 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL‑20
Leu25-Glu176
Accession # AAF36679
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑20 (Catalog # 1102-IL)
Neutralization
Measured by its ability to neutralize IL‑20-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 2 ng/mL Recombinant Human IL‑20.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑20 and Neutralization by Human IL‑20 Antibody. View Larger

Cell Proliferation Induced by IL‑20 and Neutralization by Human IL‑20 Antibody. Recombinant Human IL‑20 (Catalog # 1102-IL) stimulates proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 Ra and IL‑20 R beta in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑20 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑20 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1102). The ND50 is typically 0.1-0.4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-20

Human Interleukin 20 (IL-20) was identified by searching sequence databases for translated sequences containing a signal sequence and amphipathic helices found in helical cytokines. Human IL-20 is synthesized as a 176 amino acid (aa) precursor with a 24 aa signal sequence and a 152 aa mature segment. There are no N-linked glycosylation sites and it is doubtful that the native molecule is glycosylated. Although IL-20 is a distant member of the IL-10 family, it functions as a monomer. IL‑20 shares less than 40% aa sequence identity with other IL-10 family members. Mouse and human IL‑20 share 77% aa sequence identity in their mature segments. Human IL‑20 is active on mouse cells. IL-20 production has been found in skin and trachea. In particular, activated keratinocytes and, possibly, monocytes are reported to express IL-20. There are two heterodimeric receptor complexes for IL-20. The first is composed of IL-20 R alpha and IL-20 R beta. The second is composed of IL‑22 R and IL-20 R beta. Whereas the IL-22 R/IL-20 R beta complex is shared with IL-24/mda-7, the IL-20 R alpha /IL-20 R beta  complex is shared with both IL-19 and IL-24. Little is known about the function of IL-20. It is reported to induce the proliferation of multipotential hematopoietic progenitor cells, direct the differentiation and expansion of keratinocytes, and promote the release of proinflammatory mediators in keratinocytes and other IL-20 receptor expressing cells (1-6).

References
  1. Blumberg, H. et al. (2001) Cell 104:9.
  2. Liu, L. et al. (2003) Blood 102:3206. 
  3. Rich, B.E. and T.S. Kupper (2001) Curr. Biol. 11:R531.
  4. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. 
  5. Dumoutier, L. (2001) J. Immunol. 167:3545. 
  6. Romer, J. (2003) J. Invest. Dermatol. 121:1306.
Long Name
Interleukin 20
Entrez Gene IDs
50604 (Human); 58181 (Mouse)
Alternate Names
Cytokine Zcyto10; IL10D; IL20; IL-20; IL-20ZCYTO10four alpha helix cytokine; interleukin 20; interleukin-20; MGC96907

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Citations for Human IL-20 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Murine astrocytes are responsive to the pro-inflammatory effects of IL-20
    Authors: Amanda R. Burmeister, M. Brittany Johnson, Ian Marriott
    Neuroscience Letters
  2. IL-20 Signaling in Activated Human Neutrophils Inhibits Neutrophil Migration and Function
    Authors: P Gough, S Ganesan, SK Datta
    J. Immunol., 2017-04-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  3. Induction of phenotype modifying cytokines by FERMT1 mutations.
    Authors: Heinemann A, He Y, Zimina E, Boerries M, Busch H, Chmel N, Kurz T, Bruckner-Tuderman L, Has C
    Hum. Mutat., 2011-03-01;32(4):397-406.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis.
    Authors: Wolk K, Witte E, Warszawska K, Schulze-Tanzil G, Witte K, Philipp S, Kunz S, Docke WD, Asadullah K, Volk HD, Sterry W, Sabat R
    Eur. J. Immunol., 2009-12-01;39(12):3570-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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