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Human IL-22 ELISA Kit - Quantikine

Catalog #: D2200
43 Citations 3 Reviews Datasheet / COA / SDS
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D2200
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QC29: Quantikine Immunoassay Control Set 860 for Human IL-22
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Human IL-22 ELISA Standard Curve
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Product Details
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Citations (43)
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Supplemental Products
Reviews (3)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IL-22 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Urine (100 uL)
Sensitivity
5.8 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human IL-22
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human IL-22 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IL-22 in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human IL-22 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-22 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring IL-22.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 56.5 227 468 63 243 491
Standard Deviation 2.55 7 10.7 5.32 12.1 19.6
CV% 4.5 3.1 2.3 8.4 5 4

Recovery

The recovery of human IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 107 104-110
EDTA Plasma (n=4) 97 90-103
Heparin Plasma (n=4) 95 86-104
Serum (n=4) 92 87-99

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IL-22 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-22 ELISA Linearity

Scientific Data

N/A Human IL-22 ELISA Standard Curve View Larger

Human IL-22 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-22

IL-22 (Interleukin-22) is a cytokine that induces the production of reactive oxygen species, IL-6, IL-10, and TNF-alpha as well as neutrophil infiltration during inflammation. It also supports the integrity of epithelial barriers and induces epithelial cell proliferation during wound healing. IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is a shared component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29. IL-22 additionally binds to IL-22BP which blocks the interaction of IL-22 with IL-22 R.

Long Name:
Interleukin 22
Entrez Gene IDs:
50616 (Human); 50929 (Mouse); 500836 (Rat)
Alternate Names:
Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL22; IL-22; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human IL-22 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

43 Citations: Showing 1 - 10
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  1. Interleukin-19 Levels Are Increased in Palmoplantar Pustulosis and Reduced following Apremilast Treatment
    Authors: K Wolk, D Wilsmann-T, K Witte, TC Brembach, C Kromer, S Gerdes, K Ghoreschi, K Reich, R Mössner, R Sabat
    International Journal of Molecular Sciences, 2023-01-09;24(2):.
    Species: Human
    Sample Types: Plasma
  2. Effects of Dupilumab on Itch-Related Events in Atopic Dermatitis: Implications for Assessing Treatment Efficacy in Clinical Practice
    Authors: R Kishi, S Toyama, M Tominaga, Y Kamata, E Komiya, T Kaneko, Y Suga, K Takamori
    Cells, 2023-01-05;12(2):.
    Species: Human
    Sample Types: Serum
  3. Clinical determinants and prognostic significance of hypocapnia in acute heart failure
    Authors: M Garus, A Zdanowicz, M Fudim, R Zymli?ski, P Niewi?ski, B Paleczny, M Rosiek-Bie, G Iwanek, P Ponikowski, J Biegus
    Scientific Reports, 2022-10-07;12(1):16889.
    Species: Human
    Sample Types: Serum
  4. A role for serum cytokines and cell adhesion molecules in the non-invasive diagnosis of colorectal cancer
    Authors: O Farc, I Berindan-N, F Zaharie, L Budisan, O Zanoaga, V Cristea
    Oncology Letters, 2022-07-26;24(3):323.
    Species: Human
    Sample Types: Serum
  5. Chemokine Expression-Based Endotype Clustering of Chronic Rhinosinusitis
    Authors: U Förster-Ru, AJ Szczepek, G Pierchalla, JW Fluhr, M Artuc, T Zuberbier, C Bachert, H Olze
    Journal of personalized medicine, 2022-04-18;12(4):.
    Species: Human
    Sample Types: Tissue
  6. Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study
    Authors: C Gar, B Thorand, C Herder, C Sujana, M Heier, C Meisinger, A Peters, W Koenig, W Rathmann, M Roden, M Stumvoll, H Maalmi, T Meitinger, H Then, J Seissler, C Then
    PLoS ONE, 2022-01-06;17(1):e0262330.
    Species: Human
    Sample Types: Serum
  7. Differences in the Biomarker Profile of De Novo Acute Heart Failure versus Decompensation of Chronic Heart Failure
    Authors: S Nawrocka-M, J Biegus, M Hurkacz, M Guzik, M Rosiek-Bie, EA Jankowska, P Ponikowski, R Zymli?ski
    Biomolecules, 2021-11-16;11(11):.
    Species: Human
    Sample Types: Plasma
  8. Expression of Jejunal Taste Receptors in Women with Morbid Obesity
    Authors: L Bertran, M Portillo-C, S Martínez, C Aguilar, M Lopez-Dupl, D Riesco, J Binetti, M Vives, F Sabench, D Del Castil, C Richart, T Auguet
    Nutrients, 2021-07-16;13(7):.
    Species: Human
    Sample Types: Serum
  9. Blockade of IL-22 signaling reverses erythroid dysfunction in stress-induced anemias
    Authors: M Raundhal, S Ghosh, SA Myers, MS Cuoco, M Singer, SA Carr, SS Waikar, JV Bonventre, J Ritz, RM Stone, DP Steensma, A Regev, LH Glimcher
    Nature Immunology, 2021-03-22;0(0):.
    Species: Human
    Sample Types: Plasma
  10. Relationship between IL-8 Circulating Levels and TLR2 Hepatic Expression in Women with Morbid Obesity and Nonalcoholic Steatohepatitis
    Authors: T Auguet, L Bertran, J Binetti, C Aguilar, S Martínez, F Sabench, JM Lopez-Dupl, JA Porras, D Riesco, D Del Castil, C Richart
    Int J Mol Sci, 2020-06-11;21(11):.
    Species: Human
    Sample Types: Plasma
  11. IL22 drives cutaneous melanoma cell proliferation, migration and invasion through activation of miR-181/STAT3/AKT axis
    Authors: Y He, Y Yang, J Xu, Y Liao, L Liu, L Deng, X Xiong
    J Cancer, 2020-02-19;11(9):2679-2687.
    Species: Human
    Sample Types: Serum
  12. MUC1 as a target for CAR-T therapy in head and neck squamous cell carinoma
    Authors: Z Mei, K Zhang, AK Lam, J Huang, F Qiu, B Qiao, Y Zhang
    Cancer Med, 2019-12-04;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. lncRNA Mirt2 Is Downregulated in Ulcerative Colitis and Regulates IL-22 Expression and Apoptosis in Colonic Epithelial Cells
    Authors: G Ding, Y Ming, Y Zhang
    Gastroenterol Res Pract, 2019-10-07;2019(0):8154692.
    Species: Human
    Sample Types: Plasma
  14. Plasma levels of TNF-?, IL-6, IFN-?, IL-12, IL-17, IL-22, and IL-23 in achalasia, eosinophilic esophagitis (EoE), and gastroesophageal reflux disease (GERD)
    Authors: S Clayton, E Cauble, A Kumar, N Patil, D Ledford, N Kolliputi, MF Lopes-Vire, D Castell, J Richter
    BMC Gastroenterol, 2019-02-11;19(1):28.
    Species: Human
    Sample Types: Plasma
  15. PYY plays a key role in the resolution of diabetes following bariatric surgery in humans
    Authors: C Guida, SD Stephen, M Watson, N Dempster, P Larraufie, T Marjot, T Cargill, L Rickers, M Pavlides, J Tomlinson, JFL Cobbold, CM Zhao, D Chen, F Gribble, F Reimann, R Gillies, B Sgromo, P Rorsman, JD Ryan, RD Ramracheya
    EBioMedicine, 2019-01-11;0(0):.
    Species: Human
    Sample Types: Serum
  16. BCG Vaccination Protects against Experimental Viral Infection in Humans through the Induction of Cytokines Associated with Trained Immunity
    Authors: RJW Arts, SJCFM Moorlag, B Novakovic, Y Li, SY Wang, M Oosting, V Kumar, RJ Xavier, C Wijmenga, LAB Joosten, CBEM Reusken, CS Benn, P Aaby, MP Koopmans, HG Stunnenber, R van Crevel, MG Netea
    Cell Host Microbe, 2018-01-10;23(1):89-100.e5.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Association between cytokine profile and transcription factors produced by T cells subsets in early and late-onset preeclampsia
    Authors: VR Ribeiro, M Romao-Veig, GG Romagnoli, ML Matias, PR Nunes, VTM Borges, JC Peracoli, MTS Peracoli
    Immunology, 2017-06-19;0(0):.
    Species: Human
    Sample Types: Plasma
  18. Diminished plasma levels of common ?-chain cytokines in pulmonary tuberculosis and reversal following treatment
    Authors: NP Kumar, VV Banurekha, D Nair, S Babu
    PLoS ONE, 2017-04-27;12(4):e0176495.
    Species: Human
    Sample Types: Plasma
  19. Lacrimal gland-derived IL-22 regulates IL-17-mediated ocular mucosal inflammation
    Authors: YW Ji, SK Mittal, HS Hwang, EJ Chang, JH Lee, Y Seo, A Yeo, H Noh, HS Lee, SK Chauhan, HK Lee
    Mucosal Immunol, 2017-01-04;0(0):.
    Species: Human, Mouse
    Sample Types: Cell Culture Supernates, Tissue Homogenates
  20. Correlation of Tc17 cells at early stages after allogeneic hematopoietic stem cell transplantation with acute graft-versus-host disease
    Authors: Ketao Lan
    Int. Immunopharmacol., 2016-11-12;41(0):122-126.
    Species: Human
    Sample Types: Serum
  21. Alterations in the Fecal Microbiota of Patients with HIV-1 Infection: An Observational Study in A Chinese Population
    Sci Rep, 2016-08-01;6(0):30673.
    Species: Human
    Sample Types: Serum
  22. Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response.
    Authors: Lutay N, Hakansson G, Alaridah N, Hallgren O, Westergren-Thorsson G, Godaly G
    PLoS ONE, 2014-01-28;9(1):e86466.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Co-culture of human bronchial fibroblasts and CD4+ T cells increases Th17 cytokine signature.
    Authors: Loubaki L, Hadj-Salem I, Fakhfakh R, Jacques E, Plante S, Boisvert M, Aoudjit F, Chakir J
    PLoS ONE, 2013-12-05;8(12):e81983.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Long-lasting effects of BCG vaccination on both heterologous Th1/Th17 responses and innate trained immunity.
    Authors: Kleinnijenhuis J, Quintin J, Preijers F, Benn C, Joosten L, Jacobs C, van Loenhout J, Xavier R, Aaby P, van der Meer J, van Crevel R, Netea M
    J Innate Immun, 2013-10-30;6(2):152-8.
    Species: Human
    Sample Types: Plasma
  25. IL-7 licenses activation of human liver intrasinusoidal mucosal-associated invariant T cells.
    Authors: Tang, Xin-Zi, Jo, Juandy, Tan, Anthony, Sandalova, Elena, Chia, Adeline, Tan, Kai Chah, Lee, Kang Hoe, Gehring, Adam J, De Libero, Gennaro, Bertoletti, Antonio
    J Immunol, 2013-02-27;190(7):3142-52.
    Species: Human
    Sample Types: Whole Cells
  26. IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation.
    Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P
    PLoS Biol, 2012-09-25;10(9):e1001395.
    Species: Human
    Sample Types: Serum
  27. Interleukin-22 predicts severity and death in advanced liver cirrhosis: a prospective cohort study.
    Authors: Kronenberger B, Rudloff I, Bachmann M, Brunner F, Kapper L, Filmann N, Waidmann O, Herrmann E, Pfeilschifter J, Zeuzem S, Piiper A, Muhl H
    BMC Med, 2012-09-11;10(0):102.
    Species: Human
    Sample Types: Serum
  28. IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
    Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
    Clin. Cancer Res., 2011-11-02;17(24):7529-38.
    Species: Human
    Sample Types: Serum
  29. Complement component C5a Promotes Expression of IL-22 and IL-17 from Human T cells and its Implication in Age-related Macular Degeneration.
    Authors: Liu B, Wei L, Meyerle C, Tuo J, Sen HN, Li Z, Chakrabarty S, Agron E, Chan CC, Klein ML, Chew E, Ferris F, Nussenblatt RB
    J Transl Med, 2011-07-15;9(0):111.
    Species: Human
    Sample Types: Serum
  30. Kinetics of circulating Th17 cytokines and adipokines in psoriasis patients.
    Authors: Nakajima H, Nakajima K, Tarutani M, Morishige R, Sano S
    Arch. Dermatol. Res., 2011-06-17;303(6):451-5.
    Species: Human
    Sample Types: Serum
  31. Preclinical Evaluation of Local JAK1 and JAK2 Inhibition in Cutaneous Inflammation.
    Authors: Fridman JS, Scherle PA, Collins R, Burn T, Neilan CL, Hertel D, Contel N, Haley P, Thomas B, Shi J, Collier P, Rodgers JD, Shepard S, Metcalf B, Hollis G, Newton RC, Yeleswaram S, Friedman SM, Vaddi K
    J. Invest. Dermatol., 2011-06-16;131(9):1838-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. The IL23R R381Q Gene Variant Protects against Immune-Mediated Diseases by Impairing IL-23-Induced Th17 Effector Response in Humans.
    Authors: Di Meglio P, Di Cesare A, Laggner U
    PLoS ONE, 2011-02-22;6(2):e17160.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Activation of the aryl hydrocarbon receptor reveals distinct requirements for IL-22 and IL-17 production by human T helper cells.
    Authors: Ramirez JM, Brembilla NC, Sorg O, Chicheportiche R, Matthes T, Dayer JM, Saurat JH, Roosnek E, Chizzolini C
    Eur. J. Immunol., 2010-09-01;40(9):2450-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis.
    Authors: Wolk K, Witte E, Warszawska K, Schulze-Tanzil G, Witte K, Philipp S, Kunz S, Docke WD, Asadullah K, Volk HD, Sterry W, Sabat R
    Eur. J. Immunol., 2009-12-01;39(12):3570-81.
    Species: Human
    Sample Types: Serum
  35. Dexamethasone suppresses interleukin-22 associated with bacterial infection in vitro and in vivo.
    Authors: Ziesche E, Scheiermann P, Bachmann M, Sadik CD, Hofstetter C, Zwissler B, Pfeilschifter J, Muhl H
    Clin. Exp. Immunol., 2009-09-01;157(3):370-6.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. Prostaglandin E2 regulates Th17 cell differentiation and function through cyclic AMP and EP2/EP4 receptor signaling.
    Authors: Boniface K, Bak-Jensen KS, Li Y, Blumenschein WM, McGeachy MJ, McClanahan TK, McKenzie BS, Kastelein RA, Cua DJ, de Waal Malefyt R
    J. Exp. Med., 2009-03-09;206(3):535-48.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
    Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Allergy Clin. Immunol., 2008-12-03;123(1):59-66.e4.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. Chronically inflamed human tissues are infiltrated by highly differentiated Th17 lymphocytes.
    Authors: Pene J, Chevalier S, Preisser L, Venereau E, Guilleux MH, Ghannam S, Moles JP, Danger Y, Ravon E, Lesaux S, Yssel H, Gascan H
    J. Immunol., 2008-06-01;180(0):7423.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. IL-22 mediates mucosal host defense against Gram-negative bacterial pneumonia.
    Authors: Aujla SJ, Chan YR, Zheng M, Fei M, Askew DJ, Pociask DA, Reinhart TA, McAllister F, Edeal J, Gaus K, Husain S, Kreindler JL, Dubin PJ, Pilewski JM, Myerburg MM, Mason CA, Iwakura Y, Kolls JK
    Nat. Med., 2008-02-10;14(3):275-81.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Linking genetic susceptibility to Crohn's disease with Th17 cell function: IL-22 serum levels are increased in Crohn's disease and correlate with disease activity and IL23R genotype status.
    Authors: Schmechel S, Konrad A, Diegelmann J, Glas J, Wetzke M, Paschos E, Lohse P, Goke B, Brand S
    Inflamm. Bowel Dis., 2008-02-01;14(2):204-12.
    Species: Human
    Sample Types: Serum
  41. The role of interleukin-22 in hepatitis C virus infection.
    Authors: Dambacher J, Beigel F, Zitzmann K, Heeg MH, Goke B, Diepolder HM, Auernhammer CJ, Brand S
    Cytokine, 2008-01-11;41(3):209-16.
    Species: Human
    Sample Types: Serum
  42. IL-22 induces lipopolysaccharide-binding protein in hepatocytes: a potential systemic role of IL-22 in Crohn's disease.
    Authors: Wolk K, Witte E, Hoffmann U, Doecke WD, Endesfelder S, Asadullah K, Sterry W, Volk HD, Wittig BM, Sabat R
    J. Immunol., 2007-05-01;178(9):5973-81.
    Species: Human
    Sample Types: Cell Culture Supernates
  43. IL-22 regulates the expression of genes responsible for antimicrobial defense, cellular differentiation, and mobility in keratinocytes: a potential role in psoriasis.
    Authors: Wolk K, Witte E, Wallace E, Docke WD, Kunz S, Asadullah K, Volk HD, Sterry W, Sabat R
    Eur. J. Immunol., 2006-05-01;36(5):1309-23.
    Species: Human
    Sample Types: Plasma

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Human IL-22 Quantikine ELISA Kit
By Anonymous on 01/11/2021
Sample Tested: Heparin Plasma
Human IL-22 Quantikine ELISA Kit
By Anonymous on 11/30/2017
Sample Tested: B16-F10 mouse melanoma cell line
Human IL-22 Quantikine ELISA Kit
By Anonymous on 09/07/2017
Sample Tested: Serum

The IL-22 Quantikine ELISA performed as expected with low % CV's, and reproducible results.

Human IL-22 Quantikine ELISA Kit D2200