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Human IL-34 Antibody

Catalog #: MAB5265
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5265
MAB5265-SP
Cell Proliferation Induced by IL‑34 and Neutralization by Human IL‑34 Antibody.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-34 Antibody Summary

Species Reactivity
Human
Specificity
Detects recombinant human IL-34 in direct ELISAs.  In direct ELISAs, no cross-reactivity with recombinant mouse IL-34 is observed.
Source
Monoclonal Mouse IgG1 Clone # 578416
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-34
Asn21-Pro242
Accession # Q6ZMJ4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<1.0 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Human IL-34 transfected NS0 cells fixed with paraforaldehyde and permeabilized with saponin
Neutralization
Measured by its ability to neutralize IL‑34-induced proliferation in human peripheral blood monocytes. Lin, H. et al. (2008) Science 320:807. The Neutralization Dose (ND50) is typically 0.01-0.05 µg/mL in the presence of 10 ng/mL Recombinant Human IL‑34.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑34 and Neutralization by Human IL‑34 Antibody. View Larger

Cell Proliferation Induced by IL‑34 and Neutralization by Human IL‑34 Antibody. Recombinant Human IL-34 (Catalog # 5265-IL) stimulates proliferation in human peripheral blood monocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-34 (10 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-34 Monoclonal Antibody (Catalog # MAB5265). The ND50 is typically 0.01-0.05 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-34

Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers (1). It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level (1). IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R) (1). IL-34 stimulates monocyte proliferation (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1).

References
  1. Lin, H. et al. (2008) Science 320:807.
Long Name
Interleukin 34
Entrez Gene IDs
146433 (Human); 76527 (Mouse)
Alternate Names
C16orf77; IL34; IL-34; interleukin 34

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Citation for Human IL-34 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. IL-34 is a Treg-specific cytokine and mediates transplant tolerance.
    Authors: Bezie S, Picarda E, Ossart J, Tesson L, Usal C, Renaudin K, Anegon I, Guillonneau C
    J Clin Invest, 2015-09-21;125(10):3952-64.
    Species: Human, Rat
    Sample Types: Whole Cells
    Applications: Neutralization

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