Human IL-3R alpha/CD123 PE-conjugated Antibody

Catalog #: FAB301P
4 Citations Datasheet / COA / SDS

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FAB301P

Detection of IL‑3 R alpha /CD123 in Human Blood Lymphocytes by Flow Cytometry.

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Citations (4)

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Human IL-3R alpha/CD123 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-3 R alpha in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 32703

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-3 R alpha
Lys20-Arg305, predicted
Accession # P26951

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

Human peripheral blood lymphocytes; THP-1 human acute monocytic leukemia cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IL‑3 R alpha /CD123 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human HLA-DR APC-conjugated Monoclonal Antibody (FAB4869A) and either (A) Mouse Anti-Human IL-3 Ra/CD123 PE-conjugated Monoclonal Antibody (Catalog # FAB301P) or (B) Mouse IgG₁Phycoerythrin Isotype Control (IC002P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of IL-3R alpha/CD123 in THP-1 cells by Flow Cytometry THP-1 cells were stained with Mouse Anti-Human IL-3R alpha/CD123 PE‑conjugated Monoclonal Antibody (Catalog # FAB301P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-3R alpha

IL-3 is a pleiotropic cytokine that can stimulate proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors (1, 2). IL-3 exerts its activity through binding to a specific cell surface receptor known as IL-3 R. IL-3 R is a heterodimeric structure composed of a 70 kDa IL-3 R alpha subunit (CD123) and a 120-140 kDa IL-3 R beta subunit (CD131) (3, 4). IL-3 R alpha binds IL-3 with relatively low affinity. In the presence of IL-3 R beta, however, IL-3 R alpha has a much higher affinity for IL-3. It is not clear how signal transduction occurs following IL-3 binding. The IL-3 R alpha chain has a very short intracellular domain while the IL‑3 R beta chain has a very large cytoplasmic domain. The IL‑3 R beta chain is also shared by the receptors for IL-5 and GM-CSF. Cells known to express IL-3 receptors include hematopoietic progenitors, epithelial cells, double negative T cells, mast cells, basophils and blood monocytes (5).

References

Moore, M.A.S. et al. (1991) Blood 72:944.
Warren, D.J. et al. (1988) J. Immunol. 140:94.
Plant M. et al. (1989) Nature 339:150.
Budel, L.M. et al. (1990) Blood 75:1439.
Schrader, J.W. et al. (1988) In Interleukin-3: The Panspecific hemopoietin (ed. J.W. Schrader), Academic Press, San Diego, CA.

Long Name

Interleukin 3 Receptor alpha

Entrez Gene IDs

3563 (Human); 16188 (Mouse); 102138639 (Cynomolgus Monkey)

Alternate Names

CD123 antigen; CD123; hIL3Ra; hIL-3Ra; IL-3 R alpha; IL-3 receptor subunit alpha; IL3R alpha; IL-3R alpha; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor, alpha (low affinity); interleukin-3 receptor subunit alpha; MGC34174

Product Datasheets

Product Datasheet

COA

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Citations for Human IL-3R alpha/CD123 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Selection and characterization of antibody clones are critical for accurate flow cytometry-based monitoring of CD123 in acute myeloid leukemia
Authors: Nicole M. Cruz, Mayumi Sugita, Nathan Ewing-Crystal, Linda Lam, Roman Galetto, Agnes Gouble et al.
Leukemia & Lymphoma
Aerobic exercise in humans mobilizes HSC in an intensity dependent manner
Authors: Gianni Parise
J. Appl. Physiol., 2016-11-23;0(0):jap.00696.201.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Identification of campath-1 (CD52) as novel drug target in neoplastic stem cells in 5q-patients with MDS and AML.
Authors: Blatt K, Herrmann H, Hoermann G, Willmann M, Cerny-Reiterer S, Sadovnik I, Herndlhofer S, Streubel B, Rabitsch W, Sperr W, Mayerhofer M, Rulicke T, Valent P
Clin Cancer Res, 2014-05-05;20(13):3589-602.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R.
Authors: Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Holler C, Wacheck V, Pehamberger H, Valent P
PLoS ONE, 2014-01-29;9(1):e84417.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry