Human IL-4 Antibody Summary
His25-Ser153
Accession # P05112
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody. Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody.](https://resources.rndsystems.com/images/datasheets/antibody/IL-4_MAB204_Block_Neutralize_9058.jpg)
Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody. Recombinant Human IL-4 (Catalog # 204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-4 Monoclonal Antibody (Catalog # MAB204). The ND50 is typically 0.5-1.5 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-4
Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39%, and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5-7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on non-hematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10-13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).
- Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
- Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
- Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894.
- Redfield, C. et al. (1991) Biochemistry 30:11029.
- Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
- Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
- Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
- Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
- Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
- Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
- Corthay, A. (2006) Scand. J. Immunol. 64:93.
- Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
- Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
- Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
Product Datasheets
Citations for Human IL-4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
26
Citations: Showing 1 - 10
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RNA-sequencing and mass-spectrometry proteomic time-series analysis of T-cell differentiation identified multiple splice variants models that predicted validated protein biomarkers in inflammatory diseases
Authors: Rasmus Magnusson, Olof Rundquist, Min Jung Kim, Sandra Hellberg, Chan Hyun Na, Mikael Benson et al.
Frontiers in Molecular Biosciences
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The Cytokine IL-17A Limits Th17 Pathogenicity via a Negative Feedback Loop Driven by Autocrine Induction of IL-24
Authors: Wai Po Chong, Mary J. Mattapallil, Kumarkrishna Raychaudhuri, So Jin Bing, Sihan Wu, Yajie Zhong et al.
Immunity
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Long Intergenic Noncoding RNA MIAT as a Regulator of Human Th17 Cell Differentiation
Authors: Mohd Moin Khan, Meraj Hasan Khan, Ubaid Ullah Kalim, Sofia Khan, Sini Junttila, Niklas Paulin et al.
Frontiers in Immunology
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A dynamic single cell-based framework for digital twins to prioritize disease genes and drug targets
Authors: Xinxiu Li, Eun Jung Lee, Sandra Lilja, Joseph Loscalzo, Samuel Schäfer, Martin Smelik et al.
Genome Medicine
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Bilirubin suppresses Th17 immunity in colitis by upregulating CD39
Authors: MS Longhi, M Vuerich, A Kalbasi, JE Kenison, A Yeste, E Csizmadia, B Vaughn, L Feldbrugge, S Mitsuhashi, B Wegiel, L Otterbein, A Moss, FJ Quintana, SC Robson
JCI Insight, 2017-05-04;2(9):.
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Vitamin D Counteracts Mycobacterium tuberculosis-Induced Cathelicidin Downregulation in Dendritic Cells and Allows Th1 Differentiation and IFN gamma Secretion
Authors: Anna K. O. Rode, Martin Kongsbak, Marie M. Hansen, Daniel Villalba Lopez, Trine B. Levring, Anders Woetmann et al.
Frontiers in Immunology
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Multi-omics protein-coding units as massively parallel Bayesian networks: Empirical validation of causality structure
Authors: Alberto Zenere, Olof Rundquist, Mika Gustafsson, Claudio Altafini
iScience
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Anti-Inflammatory Effect of Specialized Proresolving Lipid Mediators on Mesenchymal Stem Cells: An In Vitro Study
Authors: S AlZahrani, Z Shinwari, A Gaafar, A Alaiya, A Al-Kahtani
Cells, 2022-12-28;12(1):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Induced Human Regulatory T Cells Express the Glucagon-like Peptide-1 Receptor
Authors: AKO Rode, TB Buus, V Mraz, FAH Al-Jaberi, DV Lopez, SL Ford, S Hennen, IP Eliasen, IV Klewe, L Gharehdagh, A Dragan, MM Rosenkilde, A Woetmann, L Skov, N Ødum, CM Bonefeld, M Kongsbak-W, C Geisler
Cells, 2022-08-19;11(16):.
Species: Human
Sample Types: Whole Cells
Applications: Stimulation -
IFNgamma-primed periodontal ligament cells regulate T-cell responses via IFNgamma-inducible mediators and ICAM-1-mediated direct cell contact
Authors: W Singhatana, S Kitpakorns, M Toso, P Pavasant
Royal Society open science, 2022-07-27;9(7):220056.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Role of CCR3 in respiratory syncytial virus infection of airway epithelial cells.
Authors: Wellemans V, Benhassou H, Fuselier E, Bellesort F, Dury S, Lebargy F, Dormoy V, Fichel C, Naour R, Gounni A, Lamkhioued B
iScience, 2021-11-14;24(12):103433.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Early-Life Stress Regulates Cardiac Development through an IL-4-Glucocorticoid Signaling Balance
Authors: DC Apaydin, PAM Jaramillo, L Corradi, F Cosco, FG Rathjen, T Kammertoen, A Filosa, S Sawamiphak
Cell Rep, 2020-11-17;33(7):108404.
Species: Zebrafish
Sample Types: In Vivo
Applications: In Vivo -
Melatonin ameliorates necrotizing enterocolitis by preventing Th17/Treg imbalance through activation of the AMPK/SIRT1 pathway
Authors: F Ma, H Hao, X Gao, Y Cai, J Zhou, P Liang, J Lv, Q He, C Shi, D Hu, B Chen, L Zhu, X Xiao, S Li
Theranostics, 2020-06-19;10(17):7730-7746.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Homeostatic Milieu Induces Production of Deoxyribonuclease 1-like 3 from Myeloid Cells
Authors: S Inokuchi, H Mitoma, S Kawano, S Nakano, M Ayano, Y Kimoto, M Akahoshi, Y Arinobu, H Tsukamoto, K Akashi, T Horiuchi, H Niiro
J. Immunol., 2020-03-18;204(8):2088-2097.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Urothelial bladder cancer may suppress perforin expression in CD8+ T cells by an ICAM-1/TGF?2 mediated pathway
Authors: CA Hartana, E Ahlén Berg, AA Zirakzadeh, D Krantz, ME Winerdal, M Winerdal, M Johansson, F Alamdari, T Jakubczyk, H Glise, K Riklund, A Sherif, O Winqvist
PLoS ONE, 2018-07-02;13(7):e0200079.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Vitamin D up-regulates the vitamin D receptor by protecting it from proteasomal degradation in human CD4+ T cells.
Authors: Kongsbak M, von Essen M, Boding L, Levring T, Schjerling P, Lauritsen J, Woetmann A, Odum N, Bonefeld C, Geisler C
PLoS ONE, 2014-05-02;9(5):e96695.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Impaired development of human Th1 cells in patients with deficient expression of STAT4.
Authors: Chang HC, Han L, Goswami R, Nguyen ET, Pelloso D, Robertson MJ, Kaplan MH
Blood, 2009-04-09;113(23):5887-90.
Species: Human
Sample Types: Whole Cells
Applications: Differentiation, Differentiation -
Normally occurring NKG2D+CD4+ T cells are immunosuppressive and inversely correlated with disease activity in juvenile-onset lupus.
Authors: Dai Z, Turtle CJ, Booth GC, Riddell SR, Gooley TA, Stevens AM, Spies T, Groh V
J. Exp. Med., 2009-03-16;206(4):793-805.
Species: Human
Sample Types: Whole Cells
Applications: Blocking -
Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells.
Authors: Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F
Nat. Immunol., 2007-08-05;8(9):942-9.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
Authors: Ebert EC, Mehta V
Cell. Immunol., 2006-08-17;241(1):7-13.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
Clin. Immunol., 2006-06-16;120(2):220-31.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Cytokine milieu of atopic dermatitis skin subverts the innate immune response to vaccinia virus.
Authors: Howell MD, Gallo RL, Boguniewicz M, Jones JF, Wong C, Streib JE, Leung DY
Immunity, 2006-03-01;24(3):341-8.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Cutting edge: immune cells as sources and targets of the IL-10 family members?
Authors: Wolk K, Kunz S, Asadullah K, Sabat R
J. Immunol., 2002-06-01;168(11):5397-402.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Progesterone Inhibits the Establishment of Activation-Associated Chromatin During TH1 Differentiation
Authors: Olof Rundquist, Colm E. Nestor, Maria C. Jenmalm, Sandra Hellberg, Mika Gustafsson
Frontiers in Immunology
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Protein interactome of the Cancerous Inhibitor of protein phosphatase 2A (CIP2A) in Th17 cells
Authors: Mohd Moin Khan, Tommi Välikangas, Meraj Hasan Khan, Robert Moulder, Ubaid Ullah, Santosh Dilip Bhosale et al.
Current Research in Immunology
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Preclinical Characterization of GLPG0634, a Selective Inhibitor of JAK1, for the Treatment of Inflammatory Diseases
Authors: Luc Van Rompaey, René Galien, Ellen M. van der Aar, Philippe Clement-Lacroix, Luc Nelles, Bart Smets et al.
The Journal of Immunology
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Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma