Human IL-4R alpha Antibody

Catalog #: AB-230-NA Datasheet / COA / SDS

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AB-230-NA

Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 R alpha Antibody.

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All IL-4R alpha Antibodies

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Human IL-4R alpha Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑4 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) IL‑5 R alpha, rhIL-5 R beta, rhIL-9 R, rhIL-13 R alpha 1, rhIL-13 R alpha 2 and recombinant mouse IL-4 R is observed.

Source

Polyclonal Goat IgG

Purification

Protein A or G purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑4 R alpha
Gly24-His232
Accession # P24394

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Purity

Protein A or G purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human IL‑4 R alpha (Catalog # 230-4R)

Neutralization

Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 5-10 µg/mL in the presence of 0.2 ng/mL Recombinant Human IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 R alpha Antibody. Recombinant Human IL-4 (Catalog # 204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-4 Ra Polyclonal Antibody (Catalog # AB-230-NA). The ND₅₀ is typically 5-10 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4R alpha

Interleukin 4 (IL-4) is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. The biological functions of IL-4 are mediated by the binding of IL‑4 to high-affinity cell surface receptor complexes. Two types of IL-4 receptor complexes have been described. The type I IL-4 receptor complex is composed of a high-affinity IL-4-binding subunit (referred to as IL-4 R alpha ) and the common gamma chain that does not bind IL-4 by itself. The type II IL-4 receptor complex is composed of IL-4 R alpha and IL-13 R alpha 1. Besides IL-4 signals, the type II IL-4 receptor complex can also transduce IL-13 signals. In the type II complex, the IL-4 R alpha subunit binds only IL-4 and not IL-13. Similarly, the IL-13 R alpha 1 subunit binds only IL-13 and not IL-4. The cDNA clones for both the human and mouse IL-4 R alpha have been isolated and shown to encode an approximately 140 kDa type I transmembrane protein with a large cytoplasmic domain that is essential for signal transduction. In mouse cells, an alternatively spliced variant encoding a soluble secreted IL-4 R alpha isoform has also been identified. Naturally occurring soluble IL-4 R alpha that binds IL-4 with high-affinity has been found in mouse and human biological fluids.

References

Keegan, A.D. (2001) in Cytokine Reference, Academic Press, Vol. 1:127.

Long Name

Interleukin 4 Receptor alpha

Entrez Gene IDs

3566 (Human); 16190 (Mouse)

Alternate Names

CD124 antigen; CD124; IL-4 R alpha; IL-4 receptor subunit alpha; IL4R alpha; IL-4R alpha; IL-4R subunit alpha; IL4R; IL-4Ra; IL4RACD124; IL-4R-alpha; interleukin 4 receptor; interleukin-4 receptor alpha chain; interleukin-4 receptor subunit alpha