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Human IL-6 Antibody

Catalog #: AF-206-NA
37 Citations 2 Reviews Datasheet / COA / SDS
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AF-206-NA
AF-206-SP
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IL‑6 in Human PBMCs.
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Citations (37)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-6 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IL-6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL-6 Pro29-Met212 Accession # P05231
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R.P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND50) is typically ≤ 125 ng/mL in the presence of 2.5 ng/mL Recombinant Human IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry IL-6 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IL‑6 in Human PBMCs. IL-6 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Goat Anti-Human IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody. View Larger

Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody. Recombinant Human IL-6 (Catalog # 206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA). The ND50 is typically ≤ 125 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length expressed as a 22-28 kDA molecular weight protein. IL-6 shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Long Name
Interleukin 6
Entrez Gene IDs
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names
B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

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Citations for Human IL-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. Citrate metabolism controls the senescent microenvironment via the remodeling of pro-inflammatory enhancers
    Authors: Etoh, K;Araki, H;Koga, T;Hino, Y;Kuribayashi, K;Hino, S;Nakao, M;
    Cell reports
    Species: Mouse, Human
    Sample Types: Chromatin, Cell Lysates, Whole Tissue
    Applications: Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Western Blot
  2. IL-33-dependent NF-?B activation inhibits apoptosis and drives chemoresistance in acute myeloid leukemia
    Authors: Yan, M;Chen, X;Ye, Q;Li, H;Zhang, L;Wang, Y;
    Cytokine
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  3. Suppression of TGF beta -Induced Interleukin-6 Secretion by Sinulariolide from Soft Corals through Attenuation of the p38-NF-kB Pathway in Carcinoma Cells
    Authors: Yang, JL;Lin, WL;Tai, SB;Ciou, YS;Chung, CL;Chen, JJ;Liu, PF;Lin, MW;Chen, CL;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  4. CCL18 signaling from tumor-associated macrophages activates fibroblasts to adopt a chemoresistance-inducing phenotype
    Authors: W Zeng, L Xiong, W Wu, S Li, J Liu, L Yang, L Lao, P Huang, M Zhang, H Chen, N Miao, Z Lin, Z Liu, X Yang, J Wang, P Wang, E Song, Y Yao, Y Nie, J Chen, D Huang
    Oncogene, 2022-11-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  5. Effects of interleukin 1beta on long noncoding RNA and mRNA expression profiles of human synovial fluid derived mesenchymal stem cells
    Authors: YP Sun, YY Lu, J Chen, JH Bao, H Zhang, JD Sun, WT Liao
    Scientific Reports, 2022-05-19;12(1):8432.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Pro-Inflammatory Cytokines Trigger the Overexpression of Tumour-Related Splice Variant RAC1B in Polarized Colorectal Cells
    Authors: JFS Pereira, C Bessa, P Matos, P Jordan
    Cancers, 2022-03-09;14(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Primary tumors release ITGBL1-rich extracellular vesicles to promote distal metastatic tumor growth through fibroblast-niche formation
    Authors: Q Ji, L Zhou, H Sui, L Yang, X Wu, Q Song, R Jia, R Li, J Sun, Z Wang, N Liu, Y Feng, X Sun, G Cai, Y Feng, J Cai, Y Cao, G Cai, Y Wang, Q Li
    Nat Commun, 2020-03-05;11(1):1211.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging
    Authors: DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé
    JCI Insight, 2019-06-11;5(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  9. Osteoblasts are "educated" by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment
    Authors: AD Kolb, AB Shupp, D Mukhopadhy, FC Marini, KM Bussard
    Breast Cancer Res., 2019-02-27;21(1):31.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  10. Human umbilical cord-derived mesenchymal stem cells direct macrophage polarization to alleviate pancreatic islets dysfunction in type 2 diabetic mice
    Authors: Y Yin, H Hao, Y Cheng, L Zang, J Liu, J Gao, J Xue, Z Xie, Q Zhang, W Han, Y Mu
    Cell Death Dis, 2018-07-09;9(7):760.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Improved efficiency of in situ protein analysis by proximity ligation using UnFold probes
    Authors: A Klaesson, K Grannas, T Ebai, J Heldin, B Koos, M Leino, D Raykova, J Oelrich, L Arngården, O Söderberg, U Landegren
    Sci Rep, 2018-03-29;8(1):5400.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Proximity Ligation Assay (PLA)
  12. Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies
    Authors: R Jalili, J Horecka, JR Swartz, RW Davis, HHJ Persson
    Proc. Natl. Acad. Sci. U.S.A., 2018-01-16;0(0):.
    Applications: Affinity Assay
  13. SorLA in interleukin-6 signaling and turnover
    Authors: JV Larsen, CM Petersen
    Mol. Cell. Biol, 2017-05-16;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. Pathomimetic avatars reveal divergent roles of microenvironment in invasive transition of ductal carcinoma in situ
    Authors: M Sameni, D Cavallo-Me, OE Franco, A Chalasani, K Ji, N Aggarwal, A Anbalagan, X Chen, RR Mattingly, SW Hayward, BF Sloane
    Breast Cancer Res., 2017-05-15;19(1):56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. Adipose Stromal Cells from Visceral and Subcutaneous Fat Facilitate Migration of Ovarian Cancer Cells via IL-6/JAK2/STAT3 Pathway
    Authors: B Kim, HS Kim, S Kim, G Haegeman, BK Tsang, DN Dhanasekar, YS Song
    Cancer Res Treat, 2016-07-18;49(2):338-349.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation.
    Authors: Procopio M, Laszlo C, Al Labban D, Kim D, Bordignon P, Jo S, Goruppi S, Menietti E, Ostano P, Ala U, Provero P, Hoetzenecker W, Neel V, Kilarski W, Swartz M, Brisken C, Lefort K, Dotto G
    Nat Cell Biol, 2015-08-24;17(9):1193-204.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Il-6 signaling between ductal carcinoma in situ cells and carcinoma-associated fibroblasts mediates tumor cell growth and migration.
    Authors: Osuala K, Sameni M, Shah S, Aggarwal N, Simonait M, Franco O, Hong Y, Hayward S, Behbod F, Mattingly R, Sloane B
    BMC Cancer, 2015-08-13;15(0):584.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC, Neutralization
  18. Fucose-based PAMPs prime dendritic cells for follicular T helper cell polarization via DC-SIGN-dependent IL-27 production.
    Authors: Gringhuis S, Kaptein T, Wevers B, van der Vlist M, Klaver E, van Die I, Vriend L, de Jong M, Geijtenbeek T
    Nat Commun, 2014-10-03;5(0):5074.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Phosphoproteomic profiling reveals IL6-mediated paracrine signaling within the Ewing sarcoma family of tumors.
    Authors: Anderson J, Titz B, Akiyama R, Komisopoulou E, Park A, Tap W, Graeber T, Denny C
    Mol Cancer Res, 2014-08-04;12(12):1740-54.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  20. Tumor-activated TCRgammadelta(+) T cells from gastric cancer patients induce the antitumor immune response of TCRalphabeta(+) T cells via their antigen-presenting cell-like effects.
    Authors: Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
    J Immunol Res, 2014-03-31;2014(0):593562.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  21. Digital microfluidic assay for protein detection.
    Authors: Mok J, Mindrinos M, Davis R, Javanmard M
    Proc Natl Acad Sci U S A, 2014-01-21;111(6):2110-5.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: ELISA Detection, ELISA Capture
  22. Inhibition of IL-6 expression in LNCaP prostate cancer cells by a combination of atorvastatin and celecoxib.
    Authors: Wang H, Cui X, Goodin S, Ding N, Van Doren J, Du Z, Huang M, Liu Y, Cheng X, Dipaola R, Conney A, Zheng X
    Oncol Rep, 2013-11-29;31(2):835-41.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  23. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
    Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
    J. Immunol. Methods, 2012-02-17;378(1):72-80.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  24. Microarray and Proteomic Analysis of Breast Cancer Cell and Osteoblast Co-cultures: ROLE OF OSTEOBLAST MATRIX METALLOPROTEINASE (MMP)-13 IN BONE METASTASIS.
    Authors: Morrison C, Mancini S, Cipollone J, Kappelhoff R, Roskelley C, Overall C
    J. Biol. Chem., 2011-07-22;286(39):34271-85.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  25. p38MAPK is a novel DNA damage response-independent regulator of the senescence-associated secretory phenotype.
    Authors: Freund A, Patil CK, Campisi J
    EMBO J., 2011-03-11;30(8):1536-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells.
    Authors: Ara T, Song L, Shimada H, Keshelava N, Russell HV, Metelitsa LS, Groshen SG, Seeger RC, DeClerck YA
    Cancer Res., 2009-01-01;69(1):329-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  27. Epigallocatechin-3-gallate inhibits IL-6 synthesis and suppresses transsignaling by enhancing soluble gp130 production.
    Authors: Ahmed S, Marotte H, Kwan K, Ruth JH, Campbell PL, Rabquer BJ, Pakozdi A, Koch AE
    Proc. Natl. Acad. Sci. U.S.A., 2008-09-16;105(38):14692-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  28. Oncogene-induced senescence relayed by an interleukin-dependent inflammatory network.
    Authors: Kuilman T, Michaloglou C, Vredeveld LC, Douma S, van Doorn R, Desmet CJ, Aarden LA, Mooi WJ, Peeper DS
    Cell, 2008-06-13;133(6):1019-31.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  29. Exploring human testicular peritubular cells: identification of secretory products and regulation by tumor necrosis factor-alpha.
    Authors: Schell C, Albrecht M, Mayer C, Schwarzer JU, Frungieri MB, Mayerhofer A
    Endocrinology, 2008-01-10;149(4):1678-86.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  30. Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
    Authors: Skibinski G, Elborn JS, Ennis M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007-03-23;293(1):L69-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  31. T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue.
    Authors: Withers DR, Fiorini C, Fischer RT, Ettinger R, Lipsky PE, Grammer AC
    Blood, 2007-02-13;109(11):4856-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  32. Interleukin-6 and vascular endothelial growth factor release by renal cell carcinoma cells impedes lymphocyte-dendritic cell cross-talk.
    Authors: Cabillic F, Bouet-Toussaint F, Toutirais O, Rioux-Leclercq N, Fergelot P, de La Pintiere CT, Genetet N, Patard JJ, Catros-Quemener V
    Clin. Exp. Immunol., 2006-12-01;146(3):518-23.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  33. Induction of interleukin-6 by hepatitis C virus core protein in hepatitis C-associated mixed cryoglobulinemia and B-cell non-Hodgkin's lymphoma.
    Authors: Feldmann G, Nischalke HD, Nattermann J, Banas B, Berg T, Teschendorf C, Schmiegel W, Duhrsen U, Halangk J, Iwan A, Sauerbruch T, Caselmann WH, Spengler U
    Clin. Cancer Res., 2006-08-01;12(15):4491-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  34. Normalizing the bone marrow microenvironment with p38 inhibitor reduces multiple myeloma cell proliferation and adhesion and suppresses osteoclast formation.
    Authors: Nguyen AN, Stebbins EG, Henson M, O&amp;apos;Young G, Choi SJ, Quon D, Damm D, Reddy M, Ma JY, Haghnazari E, Kapoun AM, Medicherla S, Protter A, Schreiner GF, Kurihara N, Anderson J, Roodman GD, Navas TA, Higgins LS
    Exp. Cell Res., 2006-04-04;312(10):1909-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  35. Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
    Authors: Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE
    Photochem. Photobiol., 2004-09-01;80(2):231-5.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  36. Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
    Authors: Sengupta J, Dhawan L, Ghosh D
    Cytokine, 2003-12-21;24(6):277-85.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P
  37. Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation.
    Authors: Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS
    J. Virol., 2003-02-01;77(3):1727-37.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Neutralization

FAQs

  1. What band size does AF-206-NA detect in Western blot?

    • AF-206-NA detects a 20-22 kDa band in lysate in Western blot.

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Human IL-6 Antibody
By Zoia Levashova on 11/01/2018
Application: MSD Assay Sample Tested: Vitreous humor Species: Cynomolgus Monkey

After labeling with Sulfo-Tag, used as a detection reagent in MSD assay (Meso Scale Diagnostics LLC). Paired with biotinylated IL-6 antibody (MAB206) as a capture reagent. A standard curve with recombinant human IL-6 from (Cat# 206-IL/CF) is shown (1.6-25,000 pg/ml).

Other Human IL-6 Antibody AF-206-NA
Human IL-6 Antibody
By Anonymous on 05/11/2018
Application: Cell Depl Sample Tested: Pancreatic cancer cells Species: Human