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Human IL-8/CXCL8 Quantikine HS ELISA Kit

Catalog #: HS800
33 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
HS800
PHS800
SS800
Control Products Available
QC41: Quantikine Immunoassay Control Group 10
Human CXCL8/IL-8 ELISA Standard Curve
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Citations (33)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IL-8/CXCL8 Quantikine HS ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity
0.4 pg/mL
Assay Range
1.0 - 64 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IL-8
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine HS Human IL-8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-8 levels in serum and plasma. It contains E. coli-expressed recombinant human IL-8 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-8 accurately. Results obtained using natural human IL-8 showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-8.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 5.5 18.7 37.1 5 19.4 39.2
Standard Deviation 0.3 0.7 2.7 0.4 1.6 3.7
CV% 5.5 3.7 7.3 8 8.2 9.4

Recovery

The recovery of IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 95 86-102
Heparin Plasma (n=4) 99 91-107
Serum (n=4) 99 85-110

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of IL-8 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CXCL8/IL-8 ELISA Linearity

Scientific Data

N/A Human CXCL8/IL-8 ELISA Standard Curve View Larger

Human CXCL8/IL-8 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-8/CXCL8

Interleukin-8 (IL-8), also known as IL-8, GCP-1, and NAP-1, is a heparin-binding 8-9 kDa member of the alpha, or CXC family of chemokines. There are at least 15 human CXC family members that all adopt a three beta -sheet/one alpha -helix structure. Most CXC chemokines show an N-terminal Glu-Leu-Arg (ELR) tripeptide motif. IL-8 circulates as a monomer, homodimer, and heterodimer with CXCL4/PF4. The monomer is considered the most bio-active, while the heterodimer can potentiate PF4 activity. IL-8 oligomerization is modulated by its interactions with matrix and cell surface glycosaminoglycans (GAGs). Mature human IL-8 shares 65-69% amino acid (aa) identity with canine, feline, and porcine IL-8. There is no IL-8 gene counterpart in rodent. 


Multiple isoforms of IL-8 are generated through both alternative splicing and differential proteolytic cleavage. In humans, alternative splicing generates an iso-form with an eleven aa substitution at the C-terminus. Proteolytic processing results in N-terminal truncation of IL-8 and is likely a cell-specific event. For example, fibroblasts and endothelial cells generate the 1-77 form by cleaving IL-8 following Glu21, while monocytes and lymphocytes generate the 6-77 form by cleaving following Leu25. These truncated forms generally show increased bioactivity, particularly through the CXCR1 receptor. IL-8 can also undergo citrullination on Arg27 of the precursor, a modification that increases its half-life and ability to induce leukocytosis. A wide variety of cells secrete IL-8 including monocytes and neutrophils, fibroblasts and keratinocytes, mast cells, visceral smooth muscle cells, dendritic cells, type II great alveolar cells, and endothelial cells. 

IL-8 bioactivity is mediated through two G-protein-coupled receptors, termed CXCR1/IL-8 RA and CXCR2/IL-8 RB. CXCR1 is 45-50 kDa in size and is used almost exclusively by IL-8. CXCR2 is 35-40 kDa in size and is used by nearly all CXC chemokines. Both CXCR1 and CXCR2 constitutively associate into functional homodimers. They can also heterodimerize, but these complexes dissociate following IL-8 binding. CXCR2 responds to low concentrations of IL-8 and is principally associated with chemotaxis and MMP-9 release. CXCR1, in contrast, responds to high concentrations of IL-8 and is associated with respiratory burst and phospholipase D2 activation. Thus, CXCR2 ligation induces leukocyte adhesion to activated vascular endothelium and migration to sites of inflammation, while CXCR1 ligation primes neutrophil antimicrobial activity. IL-8 can also form a complex with Serpin A1/alpha-1 Antitrypsin, and this prevents IL-8 interaction with CXCR1. 

In addition to its pro-inflammatory effects, IL-8 is involved in angiogenesis and the pathogenesis of atherosclerosis and cancer. It induces VEGF expression in vascular endothelial cells and functions as an autocrine factor for EC growth and angiogenesis. It is upregulated in atherosclerotic lesions and is elevated in the serum and cerebrospinal fluid following myocardial infarction. In cancer, IL-8 promotes epithelial-mesenchymal transition as well as tumor cell invasiveness and metastasis.

Long Name:
Interleukin 8
Entrez Gene IDs:
3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
Alternate Names:
3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 5 times for a total of 6 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  7.   Aspirate and wash 6 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour. Do not wash the plate.
    9. 200 µL Amplifier Solution
  9.   Add 200 µL Amplifier Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.
    10. 50 µL Stop Solution
  10. Add 50 µL of Stop Solution to each well. Read at 490 nm within 30 minutes. Set wavelength correction to 650 nm or 690 nm.

Citations for Human IL-8/CXCL8 Quantikine HS ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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  1. Trametinib-Resistant Melanoma Cells Displaying MITFhigh/NGFRlow/IL-8low Phenotype Are Highly Responsive to Alternating Periods of Drug Withdrawal and Drug Rechallenge
    Authors: Koziej, P;Kluszczynska, K;Hartman, ML;Czyz, M;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Inflammation-induced reorientation of reward versus punishment sensitivity is attenuated by Minocycline
    Authors: R De Marco, AW Barritt, M Cercignani, G Cabbai, A Colasanti, NA Harrison
    Brain, Behavior, and Immunity, 2023-04-25;0(0):.
    Species: Human
    Sample Types: Plasma
  3. Upregulation of the oestrogen target gene SIX1 is associated with higher growth speed and decreased survival in HCV-positive women with hepatocellular carcinoma
    Authors: RM Critelli, F Milosa, A Romanzi, S Lasagni, G Marcelli, L Di Marco, A Pivetti, F Schepis, D Romagnoli, S Mancarella, F Dituri, ML Martinez-C, G Giannelli, E Villa
    Oncology Letters, 2022-09-21;24(5):395.
    Species: Human
    Sample Types: Serum
  4. Circulating Pro-inflammatory Cytokines Do Not Explain Interindividual Variability in Visceral Sensitivity in Healthy Individuals
    Authors: RJ Pawlik, L Petrakova, L Brotte, H Engler, S Benson, S Elsenbruch
    Frontiers in Neuroscience, 2022-07-04;16(0):876490.
    Species: Human
    Sample Types: Plasma
  5. The involvement of copper, circular RNAs, and inflammatory cytokines in chronic respiratory disease
    Authors: Z Chen, J Zhu, H Zhou, Y Jia, H Ruan, Q Diao, M Li, L Zheng, S Yao, Y Guo, Y Zhou, Y Jiang
    Chemosphere, 2022-05-20;303(0):135005.
    Species: Human
    Sample Types: Plasma
  6. Nicotine regulates autophagy of human periodontal ligament cells through alpha7 nAchR that promotes secretion of inflammatory factors IL-1beta and IL-8
    Authors: Y Du, K Yang, Z Zhou, L Wu, L Wang, Y Chen, X Ge, X Wang
    BMC Oral Health, 2021-11-03;21(1):560.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. The proton ATPase inhibitor bafilomycin A1 reduces the release of rhinovirus C and cytokines from primary cultures of human nasal epithelial cells
    Authors: M Yamaya, X Deng, A Kikuchi, M Sugawara, N Saito, T Kubo, H Momma, T Kawase, K Nakagome, Y Shimotai, H Nishimura
    Virus research, 2021-08-21;304(0):198548.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Endurance Training Depletes Antioxidant System but Does Not Affect Endothelial Functions in Women with Abdominal Obesity: A Randomized Trial with a Comparison to Endurance-Strength Training
    Authors: M Jamka, P Bogda?ski, P Krzy?anows, A Mi?kiewicz, J Karolkiewi, M Du?-?uchow, R M?dry, A Lisowska, A Gotz-Wi?ck, S Iskakova, J Walkowiak, E M?dry
    Journal of Clinical Medicine, 2021-04-12;10(8):.
    Species: Human
    Sample Types: Whole Blood
  9. Comparison of two lipid emulsions on interleukin-1&beta, interleukin-8 and fatty acid composition in infants post gastrointestinal surgery:�a randomized trial
    Authors: MH Hanindita, R Irawan, IDG Ugrasena, IGBA Hariastawa
    F1000Research, 2020-09-24;9(0):1168.
    Species: Human
    Sample Types: Serum
  10. Exhaled Biomarkers in Idiopathic Pulmonary Fibrosis-A Six-Month Follow-Up Study in Patients Treated with Pirfenidone
    Authors: K Jaskiewicz, K Mycroft, M Maskey-War, K Paralusz, N Siemiez, P Nejman-Gry, M Barnas, R Krenke, K Gorska
    J Clin Med, 2020-08-05;9(8):.
    Species: Human
    Sample Types: EBC, Exhaled Breath Condensate (EBC)
  11. Reliability and suitability of physiological exercise response and recovery markers
    Authors: T Reichel, TK Bo beta lau, J Palmowski, K Eder, R Ringseis, FC Mooren, R Walscheid, E Bothur, S Samel, T Frech, M Philippe, K Krüger
    Sci Rep, 2020-07-17;10(1):11924.
    Species: Human
    Sample Types: Plasma
  12. Blood Levels of Free-Circulating Mitochondrial DNA in Septic Shock and Postsurgical Systemic Inflammation and Its Influence on Coagulation: A Secondary Analysis of a Prospective Observational Study
    Authors: E Schneck, F Edinger, M Hecker, N Sommer, O Pak, N Weissmann, A Hecker, M Reichert, M Markmann, M Sander, C Koch
    J Clin Med, 2020-06-30;9(7):.
    Species: Human
    Sample Types: Plasma
  13. The microRNA-34a-Induced Senescence-Associated Secretory Phenotype (SASP) Favors Vascular Smooth Muscle Cells Calcification
    Authors: E Zuccolo, I Badi, F Scavello, I Gambuzza, L Mancinelli, F Macrì, CC Tedesco, F Veglia, AR Bonfigli, F Olivieri, A Raucci
    Int J Mol Sci, 2020-06-23;21(12):.
    Species: Human
    Sample Types: Serum
  14. 17-Aminogeldanamycin Inhibits Constitutive Nuclear Factor-Kappa B (NF-kappaB) Activity in Patient-Derived Melanoma Cell Lines
    Authors: ML Hartman, M Rogut, A Mielczarek, M Wozniak, M Czyz
    Int J Mol Sci, 2020-05-26;21(11):.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. The relationship between malnutrition risk and inflammatory biomarkers in outpatient geriatric population
    Authors: P Fatyga, A Pac, M Fedyk-?uka, T Grodzicki, A Skalska
    Eur Geriatr Med, 2020-03-06;0(0):.
    Species: Human
    Sample Types: Serum
  16. Dynamics of Early Signalling Events during Fracture Healing and Potential Serum Biomarkers of Fracture Non-Union in Humans
    Authors: AN Burska, PV Giannoudis, BH Tan, D Ilas, E Jones, F Ponchel
    J Clin Med, 2020-02-11;9(2):.
    Species: Human
    Sample Types: Serum
  17. Angiogenic Effects of Human Dental Pulp and Bone Marrow-Derived Mesenchymal Stromal Cells and their Extracellular Vesicles
    Authors: G Merckx, B Hosseinkha, S Kuypers, S Deville, J Irobi, I Nelissen, L Michiels, I Lambrichts, A Bronckaers
    Cells, 2020-01-28;9(2):.
    Species: Human
    Sample Types: Serum
  18. HIV antiretroviral drugs, dolutegravir, maraviroc and ritonavir-boosted atazanavir use different pathways to affect inflammation, senescence and insulin sensitivity in human coronary endothelial cells
    Authors: M Auclair, AC Guénantin, S Fellahi, M Garcia, J Capeau
    PLoS ONE, 2020-01-23;15(1):e0226924.
    Species: Human
    Sample Types: cell culture supernatant
  19. Biological variation of immunological blood biomarkers in healthy individuals and quality goals for biomarker tests
    Authors: N Aziz, R Detels, JJ Quint, D Gjertson, T Ryner, AW Butch
    BMC Immunol., 2019-09-14;20(1):33.
    Species: Human
    Sample Types: Serum
  20. LIPUS vs. reaming in non-union treatment: Cytokine expression course as a tool for evaluation and differentiation of non-union therapy
    Authors: J Doll, A Moghaddam, V Daniel, B Biglari, R Heller, G Schmidmaie, TF Raven
    J Orthop, 2019-08-13;17(0):208-214.
    Species: Human
    Sample Types: Serum
  21. Interleukin-8 as a therapeutic target for chronic low back pain: Upregulation in human cerebrospinal fluid and pre-clinical validation with chronic reparixin in the SPARC-null mouse model
    Authors: E Krock, M Millecamps, KM Anderson, A Srivastava, TE Reihsen, P Hari, YR Sun, SH Jang, GL Wilcox, KG Belani, DS Beebe, J Ouellet, MR Pinto, LJ Kehl, L Haglund, LS Stone
    EBioMedicine, 2019-04-30;0(0):.
    Species: Human
    Sample Types: CSF
  22. Impact of constitutional TET2 haploinsufficiency on molecular and clinical phenotype in humans
    Authors: E Kaasinen, O Kuismin, K Rajamäki, H Ristolaine, M Aavikko, J Kondelin, S Saarinen, DG Berta, R Katainen, EAM Hirvonen, A Karhu, A Taira, T Tanskanen, A Alkodsi, M Taipale, E Morgunova, K Franssila, R Lehtonen, M Mäkinen, K Aittomäki, A Palotie, MI Kurki, O Pietiläine, M Hilpert, E Saarentaus, J Niinimäki, J Junttila, K Kaikkonen, P Vahteristo, RC Skoda, MRJ Seppänen, KK Eklund, J Taipale, O Kilpivaara, LA Aaltonen
    Nat Commun, 2019-03-19;10(1):1252.
    Species: Human
    Sample Types: Plasma
  23. Sex-specific effects of central adiposity and inflammatory markers on limbic microstructure
    Authors: C Metzler-Ba, JP Mole, E Leonaviciu, R Sims, EJ Kidd, B Ertefai, A Kelso-Mitc, F Gidney, F Fasano, J Evans, DK Jones, RJ Baddeley
    Neuroimage, 2019-02-05;189(0):793-803.
    Species: Human
    Sample Types: Plasma
  24. The influence of prostatic Cutibacterium acnes infection on serum levels of IL6 and CXCL8 in prostate cancer patients
    Authors: H Ugge, J Carlsson, B Söderquist, K Fall, O Andén, S Davidsson
    Infect. Agents Cancer, 2018-11-14;13(0):34.
    Species: Human
    Sample Types: Serum
  25. Pilot study of placental tissue collection, processing, and measurement procedures for large scale assessment of placental inflammation
    Authors: LA Sjaarda, KA Ahrens, DL Kuhr, TL Holland, UR Omosigho, BT Steffen, NL Weir, HK Tollman, RM Silver, MY Tsai, EF Schisterma
    PLoS ONE, 2018-05-11;13(5):e0197039.
    Species: Human
    Sample Types: Tissue Homogenates
  26. Microenvironment inflammatory infiltrate drives growth speed and outcome of hepatocellular carcinoma: a prospective clinical study
    Authors: R Critelli, F Milosa, F Faillaci, R Condello, E Turola, L Marzi, B Lei, F Dituri, S Andreani, P Sighinolfi, P Manni, A Maiorana, C Caporali, F di Benedet, M Del Buono, N De Maria, F Schepis, ML Martinez-C, G Giannelli, E Villa
    Cell Death Dis, 2017-08-24;8(8):e3017.
    Species: Human
    Sample Types: Serum
  27. Exogenous growth factors bFGF, EGF and HGF do not influence viability and phenotype of V600EBRAF melanoma cells and their response to vemurafenib and trametinib in vitro
    Authors: I Zalesna, M Osrodek, ML Hartman, M Rozanski, M Sztiller-S, K Niewinna, D Nejc, M Czyz
    PLoS ONE, 2017-08-22;12(8):e0183498.
    Species: Human
    Sample Types: Whole Cells
  28. Mindfulness and its efficacy for psychological and biological responses in women with breast cancer
    Authors: E Kenne Sare, LB Mårtensson, BA Andersson, P Karlsson, I Bergh
    Cancer Med, 2017-04-18;0(0):.
    Species: Human
    Sample Types: Serum
  29. Inflammatory thresholds and the species-specific effects of colonising bacteria in stable chronic obstructive pulmonary disease.
    Authors: Singh R, Mackay A, Patel A, Garcha D, Kowlessar B, Brill S, Donnelly L, Barnes P, Donaldson G, Wedzicha J
    Respir Res, 2014-09-14;15(0):114.
    Species: Human
    Sample Types: Sputum
  30. The expression of cytokines and chemokines in the blood of patients with severe weight loss from anorexia nervosa: an exploratory study.
    Authors: Pisetsky D, Trace S, Brownley K, Hamer R, Zucker N, Roux-Lombard P, Dayer J, Bulik C
    Cytokine, 2014-06-14;69(1):110-5.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Endothelial activation by platelets from sickle cell anemia patients.
    Authors: Proenca-Ferreira R, Brugnerotto A, Garrido V, Dominical V, Vital D, Ribeiro M, dos Santos M, Traina F, Olalla-Saad S, Costa F, Conran N
    PLoS ONE, 2014-02-13;9(2):e89012.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Circulating mitochondrial DNA increases with age and is a familiar trait: Implications for "inflamm-aging"
    Authors: Pinti M, Cevenini E, Nasi M, De Biasi S, Salvioli S, Monti D, Benatti S, Gibellini L, Cotichini R, Stazi M, Trenti T, Franceschi C, Cossarizza A
    Eur J Immunol, 2014-02-13;44(5):1552-62.
    Species: Human
    Sample Types: Plasma
  33. Association between traffic-related air pollution, subclinical inflammation and impaired glucose metabolism: results from the SALIA study.
    Authors: Teichert T, Vossoughi M, Vierkotter A, Sugiri D, Schikowski T, Schulte T, Roden M, Luckhaus C, Herder C, Kramer U
    PLoS ONE, 2013-12-10;8(12):e83042.
    Species: Human
    Sample Types: Plasma

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