Human IL-9 Antibody Summary
Gln19-Ile144
Accession # P15248
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by IL‑9 and Neutralization by Human IL‑9 Antibody. Recombinant Human IL-9 (Catalog # 209-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-9 (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF209). The ND50 is typically 2-5 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-9
Human IL-9 was originally identified as a cytokine found in the conditioned medium of a human T cell leukemia virus type I (HTLV-I) transformed T cell line that is mitogenic for the factor-dependent human megakaryoblastic leukemic cell line, M07e. The cDNA encoding this cytokine was subsequently isolated by functional expression cloning and found to be similar to the mouse T cell growth factor III/P40. This human cytokine and its murine homologue are now designated as human and mouse IL-9. Besides HTLV-I or -II transformed T cell lines, rhIL-9 is also produced by activated human PBLs. Human IL-9 was also reported to be expressed by primary and cultured Hodgkin and Reed-Sternberg (H-RS) cells derived from Hodgkin’s disease patients, suggesting a possible role for rhIL-9 in the development of the pathophysiology of Hodgkin’s disease.
Human and murine IL-9 are also capable of enhancing in vitro survival of human T cell lines as well as synergizing with Epo to support erythroid colony formation in vitro. However, the mast cell enhancing activity associated with rmIL-9 has not yet been demonstrated in the human system and no human IL-9-dependent T cell clones have been identified.
The gene for rhIL-9 has been mapped to human chromosome 5. As in the mouse system, the human IL-9 cDNA encodes a 144 amino acid residue precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a predicted molecular mass of 14 kDa. Human IL-9 contains four potential N-linked glycosylation sites and the native rhIL-9 is a highly glycosylated protein. Human and mouse IL-9 share 56% and 67% homology at the amino acid and nucleotide levels, respectively. Although murine IL-9 is active on human cells, human IL-9 is not active on mouse cells.
Product Datasheets
Citations for Human IL-9 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Targeting high circDNA2v levels in colorectal cancer induces cellular senescence and elicits an anti-tumor secretome
Authors: Wu, S;Dai, X;Xia, Y;Zhao, Q;Zhao, H;Shi, Z;Yin, X;Liu, X;Zhang, A;Yao, Z;Zhang, H;Li, Q;Thorne, RF;Zhang, S;Sheng, W;Hu, W;Gu, H;
Cell reports
Species: Human hepegivirus
Sample Types: Transduced Whole Cells
Applications: Neutralization -
Cancer genes disfavoring T�cell immunity identified via integrated systems approach
Authors: RJ Kishton, SJ Patel, AE Decker, SK Vodnala, M Cam, TN Yamamoto, Y Patel, M Sukumar, Z Yu, M Ji, AN Henning, D Gurusamy, DC Palmer, RA Stefanescu, AT Girvin, W Lo, A Pasetto, P Malekzadeh, DC Deniger, KC Wood, NE Sanjana, NP Restifo
Cell Reports, 2022-08-02;40(5):111153.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Involvement of IL-9 in the bronchial phenotype of patients with nasal polyposis.
Authors: Tsicopoulos A, Shimbara A, de Nadai P, Aldewachi O, Lamblin C, Lassalle P, Walls AF, Senechal S, Levitt RC, Darras J, Hamid Q, Wallaert B
J. Allergy Clin. Immunol., 2004-03-01;113(3):462-9.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr
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