Human Integrin alpha 2/CD49b Biotinylated Antibody
Human Integrin alpha 2/CD49b Biotinylated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Integrin alpha 2/CD49b in HT1080 Human Cell Line. Integrin alpha 2/CD49b was detected in immersion fixed HT1080 human fibrosarcoma cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Mouse Anti-Human Integrin alpha 2/CD49b Biotinylated Monoclonal Antibody (Catalog # BAM1233) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 2/CD49b
Integrin alpha 2 is one of twelve integrin family alpha subunits that share the beta 1 subunit (1‑3). Integrin alpha 2 beta 1 is the non-covalent heterodimer of 160 kDa alpha 2 (CD49b) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits and is one of six very late antigens on activated T cells, designated VLA2 (3). The alpha 2 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The 1102 amino acid (aa) human alpha 2 extracellular domain (ECD) shares 83‑89% aa sequence identity with mouse, rat, canine, bovine and equine alpha 2. The I domain-containing beta 1 integrins ( alpha 1 beta 1, alpha 2 beta 1, alpha 10 beta 1 and alpha 11 beta 1) all bind collagens, with alpha 2 beta 1 preferring collagens I‑III (4, 5). Platelet alpha 2 beta 1, also called GPIa, cooperates with another adhesion protein, GPVI, to coordinate platelet collagen binding and activation (3, 6, 7). Other alpha 2 beta 1 ligands include laminin, decorin, E-cadherin, and collagen-like regions of collectin molecules such as C1q (4). Adhesion is synergized by crosstalk with syndecan-1 or HGF R/c-Met, and antagonized by crosstalk with Integrin alpha 1 beta 1 (8‑10). In addition to expression on selected hematopoietic cells, alpha 2 beta 1 is present on a wide variety of non-hematopoietic cells (4). Mice deficient in the alpha 2 subunit have defects in innate immune responses, wound mast cell infiltration and angiogenesis, and platelet responses to collagen (6, 11, 12). In innate immunity, alpha 2 beta 1 binding to C1q initiates the complement cascade and costimulates mast cell activation, triggering neutrophil influx (4, 12).
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- Sarratt, K.L. et al. (2005) Blood 106:1268.
- Lecut, C. et al. (2005) Thromb. Haemost. 94:107.
- Vuoriluoto, K. et al. (2008) Exp. Cell Res. 314:3369.
- McCall-Culbreath, K.D. et al. (2008) Blood 111:3562.
- Abair, T.D. et al. (2008) Exp. Cell Res. 314:3593.
- Zweers, M. et al. (2006) J. Invest. Dermatol. 127:467.
- Edelson, B.T. et al. 2006) Blood 107:143.
Product Datasheets
Citation for Human Integrin alpha 2/CD49b Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Glycosylation modulates melanoma cell alpha2beta1 and alpha3beta1 integrin interactions with type IV collagen.
Authors: Stawikowski M, Aukszi B, Stawikowska R, Cudic M, Fields G
J Biol Chem, 2014-06-23;289(31):21591-604.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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