Human Integrin alpha 3/CD49c Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Integrin alpha 3/CD49c in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin alpha 3/CD49c Monoclonal Antibody (Catalog # MAB1345, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
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Integrin alpha 3/CD49c in HT1080 Human Cell Line. Integrin a3/CD49c was detected in immersion fixed HT1080 human fibrosarcoma cell line using Mouse Anti-Human Integrin a3/CD49c Monoclonal Antibody (Catalog # MAB1345) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 3/CD49c
VLA-3 (Very Late Antigen 3) is a member of the integrin family, beta 1 subfamily, of cell membrane adhesion molecules (1-3). Integrins are nondisulfide-linked transmembrane (TM) heterodimers that contain an alpha - and beta -subunit (1). VLA-3 is composed of an alpha 3 and beta 1 subunit. The alpha 3/CD49c subunit is a 130-150 kDa type I TM glycoprotein. It only associates with the beta 1 integrin subunit. It is synthesized as a 1051 amino acid (aa) precursor that undergoes proteolytic cleavage to generate a disulfide-linked 110 kDa, 843 aa extracellular heavy chain and a 30 kDa, 176 aa TM/cytoplasmic light chain (1, 4, 5, 6). The heavy chain contains seven 60 aa repeats that fold into a propeller-like structure (7). Sequences involving the first three repeats are associated with ligand binding (1). The light chain has two cytoplasmic alternate splice forms. The A form cytoplasmic domain is 52 aa, while the B form cytoplasmic domain is 37 aa (5). Human alpha 3 heavy chain is 88% aa identical to mouse heavy chain. VLA-3 is known to bind fibronectin, collagen, and laminin-1, 5, 8, 10 and 11 (1). It also binds tetraspanins such as CD9, CD63 and CD151. CD151 binding may actually stabilize VLA-3, enabling it to bind to additional factors (8).
- Tsuji, T. et al. (2004) J. Membr. Biol. 200:115.
- Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
- Kreidberg, J.A. (2000) Curr. Opin. Cell Biol. 12:548.
- Takada, Y. et al. (1991) J. Cell. Biol. 115:257.
- de Melker, A.A. et al. (1997) Lab. Invest. 76:547.
- Krokhin, O.V. et al. (2003) Biochemistry 42:12950.
- Springer, T.A. (2002) Curr. Opin. Struct. Biol. 12:802.
- Nishiuchi, R. et al. (2005) Proc. Natl. Acad. Sci. USA 102:1939.
Product Datasheets
Citations for Human Integrin alpha 3/CD49c Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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An innovative strategy to identify new targets for delivering antibodies to the brain has led to the exploration of the integrin family
Authors: C Cegarra, B Cameron, C Chaves, T Dabdoubi, TM Do, B Genêt, V Roudières, Y Shi, P Tchepikoff, D Lesuisse
PLoS ONE, 2022-09-15;17(9):e0274667.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC -
Biotin-transfer from a trifunctional crosslinker for identification of cell surface receptors of soluble protein ligands
Authors: TL Tremblay, JJ Hill
Sci Rep, 2017-04-19;7(0):46574.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Keratins Stabilize Hemidesmosomes through Regulation of beta4-Integrin Turnover.
Authors: Seltmann K, Cheng F, Wiche G, Eriksson J, Magin T
J Invest Dermatol, 2015-02-10;135(6):1609-20.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Alpha2(VIII) collagen substrata enhance endothelial cell retention under acute shear stress flow via an alpha2beta1 integrin-dependent mechanism: an in vitro and in vivo study.
Authors: Turner NJ, Murphy MO, Kielty CM
Circulation, 2006-08-14;114(8):820-9.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
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Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
