Human Integrin alpha 4 beta 7/LPAM-1 (Research Grade Abrilumab Biosimilar) Antibody

Catalog #: MAB10548 Datasheet / COA / SDS

Recombinant Monoclonal Antibody For Research Use Only

Catalog #	Availability	Size / Price	Qty
MAB10548-100
MAB10548-SP

Detection of Integrin alpha 4 beta 7 in Human PBMC by Flow Cytometry.

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All Integrin alpha 4 beta 7/LPAM-1 Antibodies

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Human Integrin alpha 4 beta 7/LPAM-1 (Research Grade Abrilumab Biosimilar) Antibody Summary

Species Reactivity

Human

Specificity

Detects Human Integrin alpha 4 beta 7/LPAM‑1 in direct ELISAs.This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Abrilumab. This product is for research use only.

Source

Recombinant Monoclonal Human IgG₁ Clone # Hu143

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human Integrin alpha4 beta7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Human PBMC

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin alpha 4 beta 7 in Human PBMC by Flow Cytometry. Human PBMC were stained with (A) Human Anti-Human Integrin a4 beta 7 (Research Grade Abrilumab Biosimilar) Monoclonal Antibody (Catalog # MAB10548) or (B) no primary antibody, followed by anti-Human IgG APC-conjugated Secondary Antibody (F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (FAB100P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 4 beta 7/LPAM-1

Abrilumab is an anti-human alpha 4 beta 7 integrin heterodimer monoclonal antibody designed for the treatment of inflammatory bowel disease, ulcerative colitis, and Crohn's disease.

Entrez Gene IDs

3676 (Human); 16402 (Mouse)

Alternate Names

269C wild type; CD49 antigen-like family member D; CD49d antigen; CD49d; CD49d/beta7; CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor; IA4; Integrin alpha 4 beta 7; integrin alpha 4; integrin alpha-4 subunit; integrin alpha-4; Integrin alpha-IV; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); LPAM1; LPAM-1; MGC90518; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha