Human Integrin alpha V beta 5 Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB2528G
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB2528G-100UG

Detection of Integrin alpha V beta 5 in MCF‑7 Human Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin alpha V beta 5 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin alpha V beta 5. Recognizes the human Integrin alpha V beta 5 heterodimer and does not recognize the alpha V subunit in association with any other beta subunits.

Source

Monoclonal Mouse IgG₁ Clone # P5H9

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HT1080 human fibrosarcoma cell line

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin alpha V beta 5 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Integrin aV beta 5 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB2528G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha V beta 5

Integrins are heterodimeric receptors comprised of an alpha and a beta subunit. Integrin alpha V (CD51) associates with several different beta subunits, but Integrin beta 5 associates exclusively with the alpha V subunit. Integrin alpha V beta 5, also known as Integrin alpha V beta S and Integrin alpha V beta 3B, is a transmembrane heterodimeric protein that functions as a receptor for Vitronectin. It is expressed on hepatoma cells, fibroblasts and carcinoma cells.

Entrez Gene IDs

3685 (Human)

Alternate Names

CD51; Integrin alpha V beta 5; integrin subunit alpha V; ITGAV; MSK8; VNRA; VTNR

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Integrin alpha V beta 5 Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Soluble bone-derived osteopontin promotes migration and stem-like behavior of breast cancer cells
Authors: GM Pio, Y Xia, MM Piaseczny, JE Chu, AL Allan
PLoS ONE, 2017-05-12;12(5):e0177640.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry